LOCUS       sequence-439403-       10579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains LbNOX under doxycycline inducible control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10579)
  AUTHORS   Pan X, Heacock ML, Abdulaziz EN, Violante S, Zuckerman AL, Shrestha 
            N, Yao C, Goodman RP, Cross JR, Cracan V
  TITLE     A genetically encoded tool to increase cellular NADH/NAD(+) ratio in
            living cells.
  JOURNAL   Nat Chem Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w.
  PUBMED    37884806
REFERENCE   2  (bases 1 to 10579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Oct 26. doi: 10.1038/s41589-023-01460-w."
COMMENT     Sequence Label: pLVX-TetOne-Puro-LbNOX-FLAG
FEATURES             Location/Qualifiers
     source          1..10579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        157..486
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      337..472
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             495..1094
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(495..514)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     991..1011
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1108..1696
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1161..1181)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1579..1590)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1903..2536
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2664..2680)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2664..2680)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2677..2699)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2688..2704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2712..2718,2719..2736,2737..2742))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2757..2778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2895..2912)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3066..3654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3146..3165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3825..4616,4617..4685))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4448..4467
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4686..4790)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    4838..4972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             5140..5773
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5820..5945
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6442..6675
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6860..6904
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7167..7282
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    7326..7460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7656..7679)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(9066..9433)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    9191..9209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9227..9245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9263..9281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9299..9317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9335..9353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9371..9389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9407..9425
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        9450..9960
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9879..9897
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             join(9979..10579,1..146)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 taaagtgcga aagcggcggg ccgaccgacg cccttgacga ttttgactta gacatgctcc
       61 cagccgatgc ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt
      121 ttgaccttga catgctcccc gggtaaacgc gcgaatgtgt gtcagttagg gtgtggaaag
      181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      241 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
      301 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
      361 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
      421 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
      481 tgcaaaacgc gaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
      541 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
      601 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
      661 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
      721 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
      781 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
      841 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
      901 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
      961 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     1021 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     1081 agcccggtgc ctgaacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga
     1141 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1201 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     1261 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     1321 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     1381 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     1441 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     1501 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     1561 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     1621 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     1681 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
     1741 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     1801 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     1861 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact
     1921 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     1981 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     2041 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     2101 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     2161 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     2221 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     2281 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     2341 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2401 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2461 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2521 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     2581 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct
     2641 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     2701 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     2761 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     2821 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2881 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2941 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3001 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3061 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3121 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3241 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3301 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3361 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3421 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3481 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3541 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3601 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3661 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3721 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3781 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3841 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3901 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3961 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     4021 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4081 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4141 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4201 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4261 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     4321 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     4381 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     4441 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     4501 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     4561 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     4621 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     4681 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4741 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4801 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa
     4861 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4921 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     4981 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     5041 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     5101 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
     5161 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     5221 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     5281 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     5341 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     5401 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     5461 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     5521 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     5581 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5641 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5701 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5761 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     5821 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     5881 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     5941 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     6001 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     6061 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     6121 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     6181 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     6241 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt
     6301 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     6361 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     6421 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     6481 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     6541 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     6601 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     6661 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     6721 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     6781 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     6841 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     6901 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     6961 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     7021 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     7081 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     7141 agtgaacgga tctcgacggt atcgccttta aaagaaaagg ggggattggg gggtacagtg
     7201 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     7261 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttatc
     7321 gacttaactt gtttattgca gcttataatg gttacaaata aggcaatagc atcacaaatt
     7381 tcacaaataa ggcatttttt tcactgcatt ctagttttgg tttgtccaaa ctcatcaatg
     7441 tatcttatca tgtctggatc tcaaatccct cggaagctgc gcctgtctta ggttggagtg
     7501 atacattttt atcactttta cccgtctttg gattaggcag tagctctgac ggccctcctg
     7561 tcttaggtta gtgaaaaatg tcactctctt acccgtcatt ggctgtccag cttagctcgc
     7621 aggggaggtg gtctggatcc gccggcaccg gtttacttgt catcgtcatc cttgtaatcc
     7681 atagatccac cagatccacc agatccacca gcgttgacgg attgggccac tttagcttgg
     7741 gcagcctggg cgagaatgtt caggtagttg aagggcctgt cgaaattggg ctggaacagc
     7801 atgtccacca tggccaggtc atcgatggtg ttctcgttct ggatgcagac ggacagggta
     7861 ttagcgctct gggacacgtc gtatttggac atcagggcgc ctcccaggat tctgtgtgta
     7921 tcggggtcga agaccaggct catgaggacg ggctcggtgg aaggcatgaa ctcgggccta
     7981 tagttgtcct ccacgatgac ctgctcggca ttgaggcctt gttgcttggc ggcggccagt
     8041 gtgaggcctg tggagacaat tgtcctgtca tagagggcca gtccggagct ggactgggtt
     8101 cccatgtact tcactgtggg cttgacgagg ttctttccga cgaggattcc ctgcctgacg
     8161 gcgttggtag cgaggggaat gtaggcgttt tggtgtgtgg ggttgtaatg gacggcggcg
     8221 gagtcgccag cagcgaaaat atcagggttg ctggacctca tgtagtcgtc ggtaatgatg
     8281 gcgccgttag gggccatgtc cactttgcct ttcagcaggt ctgtgttggg cctaaagcca
     8341 atgcagagga tggccaggtc ggtctcatag gagttcttgt ctgtcttgat tgtgaggccg
     8401 gtggcgctgt cggtgaagct ctccactgtc tcgcccagag cgagttgcac gccatggtcc
     8461 ctgtagtcct gttcgatgac gtcggtgaag tcggcatcga agtacttggg catgacccta
     8521 gccatggcgt caatcagggt gacgtcgtgg cctgttgtgg agtaggcctc ggccagttcg
     8581 gctccgatgt atccggcgcc gatgacggtg atcctcttgg cctccttggc gtcctcaatc
     8641 agagcctggg cgtgggccca attcttgcag agcttcaccc tatcgctgtc gatgccgggg
     8701 attttgggca cgataggcca gcttccggag gtcatcacca gtttgtcgta ggactcggtg
     8761 gtctgggcgt gattggtgag gtcctcgact gtgacggtct tttggtcagg gtcgatggcc
     8821 agcacgttgt ggttcatctg cacattagcg cccagcttct gcagttcttc aggggagctg
     8881 tagaagaggc cttggggatc ggccactttt ccgcccaggt agagggcgat gccacaggac
     8941 aggaaggaga tcacatcgtt cctctcgtag acggtgacct cggcgtcagg gtgctcagcg
     9001 aggatttgct tgatggcgaa ggtgccggca tgggtgcatc cgaccacggt gaccttcatg
     9061 aattctttac gagggtagga agtggtacgg aaagttggta taagacaaaa gtgttgtgga
     9121 attgaagttt actcaaaaaa tcagcactct tttataggcg ccctggttta cataagcaaa
     9181 gcttatacgt tctctatcac tgatagggag taaactggat atacgttctc tatcactgat
     9241 agggagtaaa ctgtagatac gttctctatc actgataggg agtaaactgg tcatacgttc
     9301 tctatcactg atagggagta aactccttat acgttctcta tcactgatag ggagtaaagt
     9361 ctgcatacgt tctctatcac tgatagggag taaactcttc atacgttctc tatcactgat
     9421 agggagtaaa ctcgaggtga taattccacg gggttggggt tgcgcctttt ccaaggcagc
     9481 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
     9541 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
     9601 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
     9661 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
     9721 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
     9781 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
     9841 tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct
     9901 ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag
     9961 ggggatcatc gaattaccat gtctagactg gacaagagca aagtcataaa ctctgctctg
    10021 gaattactca atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg
    10081 ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc
    10141 ctgccaatcg agatgctgga caggcatcat acccactcct gccccctgga aggcgagtca
    10201 tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc
    10261 gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa
    10321 aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg
    10381 tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca
    10441 aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgaa acaagcaatt
    10501 gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata
    10561 tgtggcctgg agaaacagc
//
