LOCUS       sequence-439406-       10239 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinA1 protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxna1-AP-His
FEATURES             Location/Qualifiers
     source          1..10239
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6820..6837
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6863..6984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6900..6919)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6954..6973
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7022..7039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7193..7781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7273..7292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7952..8743,8744..8812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8575..8594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8813..8917)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8985..9003
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9041..9063)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9065..9085)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9070..9399
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9250..9385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9312..9331
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9536..9555
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9622..10077)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9759..9780)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9971..9990
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10203..10225
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10217..10234
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10218..10234
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc caaacgccac catgccactg
     1621 ccacctctga gctctcggac actgctgctg ctgctcttgc tgctgctgag gggtgtgtgg
     1681 atagccatca gctcgccccc agctggtttg ggtccacaac ctgccttccg cacctttgtg
     1741 gccagtgact ggggcctcac tcacctggtg gttcacgaac agacaggaga ggtatatgtt
     1801 ggcgctgtga accgtattta caagctgtcg gggaacctaa ccctgcttcg ggcccatgtg
     1861 acaggccctg tagaagacaa tgagaaatgc tacccacccc ccagtgtaca gtcctgccca
     1921 catggtctgg gcagcacaga caacgtcaac aagcttctac tgctggacta tgcagccaac
     1981 cgcctgttgg cctgtggcag tgcctcacag ggcatctgcc agttcctgag gttggacgat
     2041 ctcttcaagc tgggcgagcc gcaccaccgc aaggaacact acctgtccag tgttcgagag
     2101 gctggcagca tggcgggagt gctcattgct gggccacccg gccagggcca ggccaagctc
     2161 ttcgtgggca cacccatcga tggcaaatca gaatacttcc ccactctgtc cagtcgccgg
     2221 ctcatggcca acgaggaaga tgctgacatg tttggctttg tgtaccagga tgagtttgtg
     2281 tcatctcagc tcaagatccc ttcagacaca ttgtccaagt tcccagcctt tgacatctac
     2341 tatgtgtaca gtttccgcag cgagcagttc gtatactacc tcaccctgca gctagacacg
     2401 cagcttacct cgcccgacgc cgctggcgag cacttcttca cctccaagat tgtgcggctg
     2461 tgcgtgaatg accccaagtt ctactcttac gtggagttcc ccattggctg cgagcaggct
     2521 ggcgtggagt atcggctggt gcaggacgcc tacctgagcc ggccgggaca ggccctggcc
     2581 aaacagctgg gcctggctga ggatgaggag gtgttgttca ctgtgttcgc acagggccag
     2641 aagaaccgcg tgaagccacc caaggagtcg gcgctgtgcc tgttcacact gagggccatc
     2701 aaggagaaaa tcaaggagcg catccaatcc tgctaccgtg gagaaggaaa gctctccctg
     2761 ccttggctgc tcaacaagga gctgggctgc atcaactcgc ccctgcagat tgatgacgac
     2821 ttctgcgggc aggatttcaa ccagccactg ggtggcacgg tcaccatcga gggcacaccc
     2881 ctttttgtcg acaaggaaga tggcctgact gctgtggctg cctatgacta ccaaggccgc
     2941 accgtggttt ttgctggcac tcgcagcggc cgcatccgca agattctggt ggaccttgca
     3001 aaccccagcg gcaggccggc cctggcttat gaaagtgtgg tggctcagga gggcaaccct
     3061 atcctgcgtg acctggtcct cagccccaac cgccagtacc tctatgctat gacggagaag
     3121 caggtgacgc aggtgcctgt ggaaagctgt gtccagtata cttcctgtga gctgtgcctg
     3181 gggtctcggg acccccactg tggctggtgt gtcctgcaca gcatctgctc taggcaggat
     3241 gcctgtgagc gggcagaaga gccccagcgt tttgcctctg acctgctgca gtgtgtgcag
     3301 ttaactgtcc agccgcgcaa tgtgtcagtc accatgtccc aggtgccgct cgtgctgcag
     3361 gcttggaatg tgcctgacct ctcagctggt gtcaactgct cctttgagga cttcacggag
     3421 actgagagca tcctggaaga tggccgcatc cattgccact caccttctgc ccgggaagtg
     3481 gcgcccatca cacagggtca gggagaccag cgggtggtga agctctacct caagtccaag
     3541 gagacaggga agaagtttgc atctgtggac tttgtcttct ataactgcag tgttcaccag
     3601 tcctgcctgg catgtgtcaa cggctccttc ccctgccatt ggtgcaaata ccgccatgtt
     3661 tgcaccaaca atgcggctga ctgcgccttt ctggaaggcc gagtcaacat gtctgaggac
     3721 tgcccacaga tcctgccttc tacccacatc tatgtgccag tgggtgtggt gaagcccatc
     3781 accctggctg cacggaacct gccgcagcca cagtcgggtc agcgagggta cgagtgcctt
     3841 ttccacatcc caggcagccc ggcccgagtc actgcactgc gcttcaacag ctccagcctg
     3901 cagtgccaga actcctcgta ctcctatgag gggaatgatg tcagcgatct gcctgtgaac
     3961 ctgtcggttg tgtggaatgg caactttgtc attgacaacc cccagaacat ccaggcccac
     4021 ctctacaagt gcccagcctt gcgccagagt tgtggcctct gcctcaaggc agacccacgc
     4081 tttgagtgcg gctggtgcgt agctgagcgc cgctgttccc ttcgtcacca ctgcccagct
     4141 gactcaccag cctcctggat gcatgcccac catggcagca gccgctgcac agaccccaag
     4201 attctcaagc tgtccccaga gacaggccct cggcagggag ggactcgact aaccatcacc
     4261 ggtgagaatc tgggcctccg gtttgaggat gtgcggctag gcgtgcacgt gggcaaggtg
     4321 ctttgcagcc ctgtggagag cgagtacatc agcgcagaac agattgtctg cgagattgga
     4381 gatgccagca cgctgcgggc ccatgatgcc ctggtggagg tgtgtgtgcg ggactgctcc
     4441 ctccactacc gagcgctgtc ccctaaacgc ttcacttttg tgacaccgac cttctaccgt
     4501 gtgagcccct cccgtgggcc tctgtctgga ggtacctgga ttggcatcga agggagccat
     4561 ctgaatgctg gcagtgatgt agctgtgtcc attggcggtc ggccctgctc cttctcctgg
     4621 agaaactcac gagagatccg ctgcctgaca ccgcccgggc acactcctgg cagcgcccct
     4681 attgttatca acatcaatcg tgcccagctc tccaaccccg aggtgaagta caactatact
     4741 gaagacccta ctatcctgag gatcgaccct gagtggagca tcaatagtgg tgggaccctc
     4801 ctaacagtca cgggcaccaa cctggccact gtccgtgaac ctcgaatccg cgccaagtat
     4861 ggaggcattg agagggaaaa tagctgcatg gtgtacaatg acaccaccat ggtgtgccgg
     4921 gcgccgtcta tagacaaccc aaagcgcagc ccgccagaac tgggagagcg gccagatgag
     4981 attggtttca tcatggacaa tgtgcgcacg ctgctggtac tcaacagctc ctccttcctc
     5041 tactacccgg accctgtact ggagccactt agccctactg ggctcttgga gctgaagccc
     5101 agctccccgc tcatcctcaa ggggcggaat ctcctgccac ctgctcctgg caattcccgg
     5161 ctcaactaca cggtgctcat cggctccaca ccttgtatcc tcaccgtgtc tgagacacag
     5221 ctgctgtgtg aagcacccaa cctcacgggg cagcacaaag tcacggtgcg ggccgggggc
     5281 ttcgagttct cacctgggat gctgcaggtg tattcggaca gcctgctgac actgactagt
     5341 ggatcttccg gaatcatccc agttgaggag gagaacccgg acttctggaa ccgcgaggca
     5401 gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc caagaacctc
     5461 atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag gatcctaaaa
     5521 gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg cttcccatat
     5581 gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg agccacagcc
     5641 acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag tgcagccgcc
     5701 cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat gaatcgggcc
     5761 aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca cgcctcgcca
     5821 gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga cgtgcctgcc
     5881 tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa catggacatt
     5941 gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc agaccctgag
     6001 tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct ggtgcaggaa
     6061 tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct catgcaggct
     6121 tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga catgaaatac
     6181 gagatccacc gagactccac actggacccc tccctgatgg agatgacaga ggctgccctg
     6241 cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg tcgcatcgac
     6301 catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat gttcgacgac
     6361 gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct cgtcactgcc
     6421 gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc catcttcggg
     6481 ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata cggaaacggt
     6541 ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga gagcgggagc
     6601 cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc aggcgaggac
     6661 gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca ggagcagacc
     6721 ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc ctgcgacctg
     6781 gcgccccccg ccggcaccac cgacgccgcg cacccgggtc atcatcacca tcaccattaa
     6841 tctagagatc ttattaaagc agaacttgtt tattgcagct tataatggtt acaaataaag
     6901 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6961 gtccaaactc atcaatgtat cttatcatgt ctggtcgact ctagctagac tcttccgctt
     7021 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7081 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7141 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7201 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7261 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7321 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7381 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7441 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7501 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7561 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7621 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7681 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7741 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7801 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7861 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7921 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7981 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8041 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8101 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8161 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8221 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8281 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8341 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8401 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8461 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8521 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8581 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8641 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8701 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8761 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8821 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8881 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8941 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9001 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     9061 cggaggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     9121 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     9241 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9301 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     9361 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     9421 tccattttcg tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     9481 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     9541 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     9601 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     9661 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     9721 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     9781 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     9841 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     9901 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     9961 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
    10021 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca
    10081 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
    10141 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
    10201 ttcccagtca cgacgttgta aaacgacggc cagtgaatt
//
