LOCUS       sequence-439408-       10222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinA2 protein, 
            C-terminally fused to alkaline phosphatase + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxna2-AP-His
FEATURES             Location/Qualifiers
     source          1..10222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6803..6820
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6846..6967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6883..6902)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6937..6956
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7005..7022)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7176..7764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7256..7275)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7935..8726,8727..8795))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8558..8577
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8796..8900)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8968..8986
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9024..9046)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9048..9068)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9053..9382
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9233..9368
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9295..9314
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9519..9538
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9605..10060)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9742..9763)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9954..9973
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10186..10208
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10200..10217
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10201..10217
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggagcagag
     1621 gaggttctat ctgcgggcca tgcaggctga caacctgtct gtggttctgc tctcagtggc
     1681 ctggctcctg ctagctcgtg ggaccacagg tatgcctcag tacagcactt tccactctga
     1741 gaatcgtgac tggactttca accatttgac tgtacaccga agaacagggg ctgtgtatgt
     1801 gggggctatc aatcgtgtct acaagttgac tggcaacctc accatccagg tggctcacaa
     1861 gacagggcca gaagaggaca acaaggcttg ctacccaccc ctcattgtac agccctgcag
     1921 tgaagtgctt acactcacca acaatgtcaa caaactactg atcattgact actctgagaa
     1981 tcgcctgctg gcctgcggaa gcctctacca gggtgtttgc aagctcctgc gactagatga
     2041 cctcttcatc ctggtggagc catcccacaa gaaggaacac tacttgtcca gtgtcaataa
     2101 gacaggcacc atgtatggtg tgattgtgcg ctctgagggg gaagatggca agctttttat
     2161 cggcactgct gtggatggca agcaggatta cttccctact ctgtccagcc gcaagctgcc
     2221 ccgtgaccct gagtcttcag caatgctgga ctatgagctc cacagtgatt ttgtctcctc
     2281 cctcatcaag attccctctg acaccctagc cctggtctct cacttcgaca tcttctacat
     2341 ctatggcttt gccagtgggg ggtttgtcta ctttctcact gtccagccag agacccctga
     2401 cggcatggcc atcaattcag ctggagacct cttctatacc tcaagaattg tgcgtctctg
     2461 caaggatgac cccaagttcc actcctatgt gtccctgcct tttggctgca cacgtgctgg
     2521 ggtggaatat cgccttctgc aggcagctta ccttgcaaag ccaggggaag ctctagctca
     2581 ggccttcaac atcagcagcg acgaagatgt cctgtttgcc atcttctcca aggggcagaa
     2641 gcagtaccac cacccccctg atgactctgc cctctgtgcc ttccccatcc gggccatcaa
     2701 cttgcaaatc aaggagcggt tgcagtcctg ctaccatgga gagggcaact tggagctcaa
     2761 ctggctgctg ggaaaggatg tgcagtgcac caaggcgcct gtcccaatcg atgataactt
     2821 ctgcggcctg gacatcaacc agcctctggg aggctccact cctgtggagg gactgaccct
     2881 gtataccacc agcagggacc gcctgacctc tgtggcctcc tatgtttaca atggctacag
     2941 tgtggttttt gtggggacta agagtggcaa gctgaagaag attcgagctg atggtccccc
     3001 ccatggtggg gtccagtatg agatggtctc tgtgttcaaa gatgggagcc caatcctccg
     3061 ggacatggcc ttctccatca atcagctata cctatatgtc atgtctgaga gacaggtcac
     3121 cagggtccct gttgaatcat gtgaacagta tacaacttgt ggagagtgtc taagctcagg
     3181 ggatcctcac tgtggctggt gtgccctgca caacatgtgc tcccgaagag acaaatgcca
     3241 acgggcctgg gaagcaaatc gatttgctgc cagtatcagc cagtgcatga gccttgaggt
     3301 acaccccaac agcatctctg tgtcagatca cagccggctg ctcagcctgg ttgtgaatga
     3361 tgctcccaac ctctctgaag gtattgcttg tgcctttggg aatctgactg aggtggaggg
     3421 acaggtatct gggagtcaag tcatctgcat ctcacctgga cccaaggatg tccctgtcat
     3481 ccctctggat caagactggt ttggcctaga gctgcagctg agatccaaag agacaggaaa
     3541 gatctttgtc agcaccgaat tcaagttcta taactgcagt gcccaccaac tgtgcctgtc
     3601 ctgtgttaac agcgccttcc gctgccattg gtgcaagtac cgtaacctct gcacacatga
     3661 ccccactacc tgttccttcc aggaaggcag gatcaatgtt tcagaggact gtccccagct
     3721 cgtgcccacg gaggagattc tgatcccagt tggggaagta aaaccaatca cccttaaggc
     3781 ccgaaacctg ccccagcccc agtctggcca gcgaggctac gagtgtgtgc tcagcattca
     3841 aggggctgtc caccgggtcc ctgccctgcg tttcaacagt tccagtgtgc agtgccaaaa
     3901 cagctcgtac cagtatgatg ggatggacat cagcaaccta gcagtggact ttgctgtagt
     3961 atggaatggc aacttcatta ttgacaaccc tcaggacctg aaagtacatc tctacaagtg
     4021 tgcagcccag cgggaaagct gtggtctctg cctcaaggct gaccacaagt tcgagtgtgg
     4081 ctggtgcagt ggtgagcgca gatgtaccct ccaccagcac tgccccagca cttctagccc
     4141 ctggcttgac tggtccagcc acaatgtcaa gtgttccaac ccccaaatca cagagatttt
     4201 gacagtatca ggaccacctg aaggagggac tcgtgtgacc atccatggcg tgaacctggg
     4261 cttggacttc tctgagattg ctcaccatgt gcaggtggct ggagtgccct gcacacctat
     4321 cccaggggaa tacatcatcg ctgagcagat cgtctgtgag atgggccatg ccgttatagg
     4381 taccacatct gggcctgtgc gcctgtgcat tggggaatgc aagccagagt tcatgaccaa
     4441 gtcccaccag cagtatactt ttgtgaatcc ttctgtgctg tcactcagcc cgatccgggg
     4501 accagagtca ggaggtacca tggtgaccat cacaggccat taccttggtg ctgggagcag
     4561 tgtggcagtg tacctgggca atcagacctg tgaattctat gggaggtcaa tgaatgagat
     4621 tgtatgtgtt tcacccccat catccaatgg actaggacca gtccctgtct ccgtgagtgt
     4681 cgacagagcc cgggtggata gcagtctgca gttcgagtat atagatgacc cacgggtcca
     4741 acgtattgag ccagagtgga gtatcactag tgggcacaca cccctaacca tcacaggctt
     4801 caacttggat gtcattcagg agcccagggt ccgagtcaaa tttaatggca aagaatctgt
     4861 caatgtatgc acagtggtaa acacaaccac cctcacctgt ctggcaccct ctctgaccag
     4921 tgactaccgc ccaggtctgg acactgtgga acggccagat gagtttggat ttctctttaa
     4981 caatgttcaa tccttactca tctataacga caccaagttc atctactacc ccaacccaac
     5041 gtttgaactg ctcagcccca ctggaatctt ggatcagaag ccaggctcac ccatcatcct
     5101 gaagggcaaa aatctctgtc ctcctgcctc tggaggggcc aaactcaact acacagtaat
     5161 gattggagag acaccttgta cagtcactgt gtctgagaca cagctgcttt gtgaacctcc
     5221 caacctcaca gggcagcaca aggtcatggt tcacgtgggc gggatggtgt tctcacctgg
     5281 ctccgtgagc gtcatctccg acagcctgtt gaccctgtct agtggatctt ccggaatcat
     5341 cccagttgag gaggagaacc cggacttctg gaaccgcgag gcagccgagg ccctgggtgc
     5401 cgccaagaag ctgcagcctg cacagacagc cgccaagaac ctcatcatct tcctgggcga
     5461 tgggatgggg gtgtctacgg tgacagctgc caggatccta aaagggcaga agaaggacaa
     5521 actggggcct gagatacccc tggccatgga ccgcttccca tatgtggctc tgtccaagac
     5581 atacaatgta gacaaacatg tgccagacag tggagccaca gccacggcct acctgtgcgg
     5641 ggtcaagggc aacttccaga ccattggctt gagtgcagcc gcccgcttta accagtgcaa
     5701 cacgacacgc ggcaacgagg tcatctccgt gatgaatcgg gccaagaaag cagggaagtc
     5761 agtgggagtg gtaaccacca cacgagtgca gcacgcctcg ccagccggca cctacgccca
     5821 cacggtgaac cgcaactggt actcggacgc cgacgtgcct gcctcggccc gccaggaggg
     5881 gtgccaggac atcgctacgc agctcatctc caacatggac attgacgtga tcctaggtgg
     5941 aggccgaaag tacatgtttc gcatgggaac cccagaccct gagtacccag atgactacag
     6001 ccaaggtggg accaggctgg acgggaagaa tctggtgcag gaatggctgg cgaagcgcca
     6061 gggtgcccgg tatgtgtgga accgcactga gctcatgcag gcttccctgg acccgtctgt
     6121 gacccatctc atgggtctct ttgagcctgg agacatgaaa tacgagatcc accgagactc
     6181 cacactggac ccctccctga tggagatgac agaggctgcc ctgcgcctgc tgagcaggaa
     6241 cccccgcggc ttcttcctct tcgtggaggg tggtcgcatc gaccatggtc atcatgaaag
     6301 cagggcttac cgggcactga ctgagacgat catgttcgac gacgccattg agagggcggg
     6361 ccagctcacc agcgaggagg acacgctgag cctcgtcact gccgaccact cccacgtctt
     6421 ctccttcgga ggctaccccc tgcgagggag ctccatcttc gggctggccc ctggcaaggc
     6481 ccgggacagg aaggcctaca cggtcctcct atacggaaac ggtccaggct atgtgctcaa
     6541 ggacggcgcc cggccggatg ttaccgagag cgagagcggg agccccgagt atcggcagca
     6601 gtcagcagtg cccctggacg aagagaccca cgcaggcgag gacgtggcgg tgttcgcgcg
     6661 cggcccgcag gcgcacctgg ttcacggcgt gcaggagcag accttcatag cgcacgtcat
     6721 ggccttcgcc gcctgcctgg agccctacac cgcctgcgac ctggcgcccc ccgccggcac
     6781 caccgacgcc gcgcacccgg gtcatcatca ccatcaccat taatctagag atcttattaa
     6841 agcagaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6901 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6961 tatcttatca tgtctggtcg actctagcta gactcttccg cttcctcgct cactgactcg
     7021 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7081 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7141 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7201 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7261 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7321 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7381 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7441 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7501 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7561 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7621 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7681 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7741 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7801 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7861 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7921 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7981 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8041 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8101 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8161 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8221 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8281 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8341 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8401 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8461 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8521 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8581 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8641 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8701 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8761 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8821 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8881 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8941 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     9001 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagga atgtgtgtca
     9061 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9121 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9181 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     9241 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9301 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     9361 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcgtcccgga
     9421 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     9481 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     9541 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     9601 caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     9661 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     9721 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     9781 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     9841 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     9901 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     9961 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
    10021 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggctgc
    10081 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    10141 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    10201 gtaaaacgac ggccagtgaa tt
//
