LOCUS       sequence-439416-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            PRDM9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-PRDM9
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3084..3108)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3110..3136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3303..3437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3709..3743
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3754..3771)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3754..3770)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3763..3785)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3979..3998)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4100..4122
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4161..4179)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4247..4351
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4352..4420,4421..5212)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4570..4589)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5383..5971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5872..5891
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6125..6142
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6348..6369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6384..6389,6390..6407,6408..6414)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6422..6438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6427..6449
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6446..6462
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6446..6462
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   6488..6550
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6726..6807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7524..7580)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7590..8333)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(8334..8357)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      8357..8366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8386..8597)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        8598..8977
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagcagg ctgccaccat gagccctgaa aagtcccaag
      421 aggagagccc agaagaagac acagagagaa cagagcggaa gcccatggtc aaagatgcct
      481 tcaaagacat ttccatatac ttcaccaagg aagaatgggc agagatggga gactgggaga
      541 aaactcgcta taggaatgtg aaaaggaact ataatgcact gattactata ggtctcagag
      601 ccactcgacc agctttcatg tgtcaccgaa ggcaggccat caaactccag gtggatgaca
      661 cagaagattc tgatgaagaa tggaccccta ggcagcaagt caaacctcct tggatggcct
      721 taagagtgga acagcgtaaa caccagaagg gaatgcccaa ggcgtcattc agtaatgaat
      781 ctagtttgaa agaattgtca agaacagcaa atttactgaa tgcaagtggc tcagagcagg
      841 ctcagaaacc agtgtcccct tctggagaag caagtacctc tggacagcac tcaagactaa
      901 aactggaact caggaagaag gagactgaaa gaaagatgta tagcctgcga gaaagaaagg
      961 gtcatgcata caaagaggtc agcgagccgc aggatgatga ttacctctat tgtgagatgt
     1021 gtcagaactt cttcattgac agctgtgctg cccatgggcc ccctacattt gtaaaggaca
     1081 gtgcagtgga caaggggcac cccaaccgtt cagccctcag tctgccccca gggctgagaa
     1141 ttgggccatc aggcatccct caggctgggc ttggagtatg gaatgaggca tctgatctgc
     1201 cgctgggtct gcactttggc ccttatgagg gccgaattac agaagacgaa gaggcagcca
     1261 acaatggata ctcctggctg atcaccaagg ggagaaactg ctatgagtat gtggatggaa
     1321 aagataaatc ctgggccaac tggatgaggt atgtgaactg tgcccgggat gatgaagagc
     1381 agaacctggt ggccttccag taccacaggc agatcttcta tagaacctgc cgagtcatta
     1441 ggccaggctg tgaactgctg gtctggtatg gggatgaata cggccaggaa ctgggcatca
     1501 agtggggcag caagtggaag aaagagctca tggcagggag agaaccaaag ccagagatcc
     1561 atccatgtcc ctcatgctgt ctggcctttt caagtcagaa atttctcagt caacatgtag
     1621 aacgcaatca ctcctctcag aacttcccag gaccatctgc aagaaaactc ctccaaccag
     1681 agaatccctg cccaggggat cagaatcagg agcagcaata tccagatcca cacagccgta
     1741 atgacaaaac caaaggtcaa gagatcaaag aaaggtccaa actcttgaat aaaaggacat
     1801 ggcagaggga gatttcaagg gccttttcta gcccacccaa aggacaaatg gggagctgta
     1861 gagtgggaaa aagaataatg gaagaagagt ccagaacagg ccagaaagtg aatccaggga
     1921 acacaggcaa attatttgtg ggggtaggaa tctcaagaat tgcaaaagtc aagtatggag
     1981 agtgtggaca aggtttcagt gttaaatcag atgttattac acaccaaagg acacatacag
     2041 gggagaagct ctacgtctgc agggagtgtg ggcggggctt tagctggaag tcacacctcc
     2101 tcattcacca gaggatacac acaggggaga agccctatgt ctgcagggag tgtgggcggg
     2161 gctttagctg gcagtcagtc ctcctcactc accagaggac acacacaggg gagaagccct
     2221 atgtctgcag ggagtgtggg cggggcttta gccggcagtc agtcctcctc actcaccaga
     2281 ggagacacac aggggagaag ccctatgtct gcagggagtg tgggcggggc tttagccggc
     2341 agtcagtcct cctcactcac cagaggagac acacagggga gaagccctat gtctgcaggg
     2401 agtgtgggcg gggctttagc tggcagtcag tcctcctcac tcaccagagg acacacacag
     2461 gggagaagcc ctatgtctgc agggagtgtg ggcggggctt tagctggcag tcagtcctcc
     2521 tcactcacca gaggacacac acaggggaga agccctatgt ctgcagggag tgtgggcggg
     2581 gctttagcaa taagtcacac ctcctcagac accagaggac acacacaggg gagaagccct
     2641 atgtctgcag ggagtgtggg cggggctttc gcgataagtc acacctcctc agacaccaga
     2701 ggacacacac aggggagaag ccctatgtct gcagggagtg tgggcggggc tttagagata
     2761 agtcaaacct cctcagtcac cagaggacac acacagggga gaagccctat gtctgcaggg
     2821 agtgtgggcg gggctttagc aataagtcac acctcctcag acaccagagg acacacacag
     2881 gggagaagcc ctatgtctgc agggagtgtg ggcggggctt tcgcaataag tcacacctcc
     2941 tcagacacca gaggacacac acaggggaga agccctacgt ctgcagggag tgtgggcggg
     3001 gctttagcga taggtcaagc ctctgctatc accagaggac acacacaggg gagaagccct
     3061 acgtctgcag ggaggatgag taaacccagc tttcttgtac aaagtggtat acccatacga
     3121 tgttccagat tacgcttagt aatgagaaat actcagaaga tgtcactcac catagtccgc
     3181 agtgaaagat aaatgatccc aggaactatc tattcttcgt tttagagcta gaaatagcaa
     3241 gtaggccact gagcaagaac tatgaatgag atctttagac atattgcggc cgctttctcc
     3301 gcgatccaga catgataaga tacattgatg agtttggaca aaccaaaact agaatgcagt
     3361 gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc cttatttgta accattataa
     3421 gctgcaataa acaagttatc tataacaaga aaatatatat ataataagtt atcacgtaag
     3481 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     3541 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     3601 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     3661 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     3721 ttacgcagac tatctttcta gggttaagaa ttcactggcc gtcgttttac aacgtcgtga
     3781 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3841 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3901 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3961 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     4021 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     4081 acaagctgtg accatgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     4141 aaacacgcga gttcgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4201 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4261 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4321 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4381 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4441 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4501 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4561 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4621 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4681 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4741 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4801 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4861 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4921 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4981 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5041 tgataaatct ggagccggtg agcgtggttc acgcggtatc attgcagcac tggggccaga
     5101 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5161 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5221 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5281 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5341 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5401 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5461 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5521 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5581 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5641 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5701 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5761 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5821 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5881 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5941 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6001 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6061 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6121 gcgcagcgag tcagtgagcg aggaagcgga tgagcgccca atacgcaaac cgcctctcac
     6181 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6241 cagtgagcgc aacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6301 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6361 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6421 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaaggtc
     6481 gacttaaccc tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa
     6541 ttgacgcatg tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat
     6601 taagttttat tatatttaca cttacatact aataataaat tcaacaaaca atttatttat
     6661 gtttatttat ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac
     6721 ttttagcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     6781 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     6841 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     6901 gctgattatg atcctctgga gattcatgcg ccaggttttc tggtcatgca ccacgttcgt
     6961 gggccctcag gaacctccac gtcagcggta actgtgaagc ccagtcgctc gtaaaaagga
     7021 agattccggg gagcactggt ttcgaggaac gccggcacgc cagcccgttc cgcggcctca
     7081 acaccaggaa gaacgacagc gcttcccaaa cctttacctt ggtgatctgg gctgacaccg
     7141 acagtggcaa gaaaccacgc aggctccttt ggtctatgag gagccagcaa gccctccatt
     7201 tgctgttgtg ccgccaacct actccctgac agctcggcca tccgcggacc tatttccgcg
     7261 aacactgcgc cggcttcgac gctttccgga gtagtccaga ccgcaactgc ggcaccatcg
     7321 tccgcaaccc aaactttacc aatgtccaga cccacgcgcg taagaaacag ctcttgcaac
     7381 tcagttaccc tctctatgtg tctgtccgga tctacggtat ggcgggtcgc tggataatct
     7441 gcaaatgctg cggcaagtgt tcggacagcc cgtggtacat cgtcacgggt agcgagccta
     7501 acagtgggct tatattccgt catggggccg gggttctcct ccacgtcgcc ggcctgcttc
     7561 agcaggctga agttggtggc gccgctgccc ccggggagca tgtcaaggtc aaaatcgtca
     7621 agagcgtcag caggcagcat atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg
     7681 tctaagtcaa aatcgtcaag ggcgtcggtc ggcccgccgc tttcgcactt tagctgtttc
     7741 tccaggccac atatgattag ttccaggccg aaaaggaagg caggttcggc tccctgccgg
     7801 tcgaacagct caattgcttg tttcagaagt gggggcatag aatcggtggt aggtgtctct
     7861 ctttcctctt ttgctacttg atgctcctgt tcctccaata cgcagcccag tgtaaagtgg
     7921 cccacggcgg acagagcgta cagtgcgttc tccagggaga agccttgctg acacaggaac
     7981 gcgagctgat tttccagggt ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact
     8041 ttagccccgt cacgatgtga gaggagagca cagcggtatg acttggcgtt gttccgcaga
     8101 aagtcttgcc atgactcgcc ttccaggggg caggagtggg tatgatgcct gtccagcatc
     8161 tcgattggca gggcatcgag cagggcccgc ttgttcttca cgtgccagta cagggtaggc
     8221 tgctcaactc ccagcttttg agcgagtttc cttgtcgtca ggccttcgat accgactcca
     8281 ttgagtaatt ccagagcaga gtttatgact ttgctcttgt ccagtctaga catcttatcg
     8341 tcatcgtctt tgtaatccat ggtggcggat cccgcgtcac gacacctgtg ttctggcggc
     8401 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc
     8461 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca
     8521 ccttctctag gcaccggttc aattgccgac ccctcccccc aacttctcgg gcactgtggg
     8581 ccatgtgcgc tctgccccat ggtaatagcc atgactaata cgtagatgta ctgccaagta
     8641 ggaaagtgcc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     8701 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     8761 taccgtaaat actccaccca ttgacgtcaa tggaaagtgc ctattggcgt tactatggga
     8821 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     8881 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     8941 attgattact attaataact agtcaataat caatgtcata agctctaatc gcataatcga
     9001 gctaagagcg attaatatgc ttagctagct tagctaagct agatttctct attctcccac
     9061 aatatatagc agctattagc ttagcagtcc gaatacgatc gtccacgatc cataagcgta
     9121 acacatttga gtagcgactg acgggcaccg gagccactcg agtggaatt
//
