LOCUS       sequence-439418-        9447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinA1 protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxna1-Fc-His
FEATURES             Location/Qualifiers
     source          1..9447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6028..6045
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6071..6192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6108..6127)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6162..6181
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6230..6247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6401..6989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6481..6500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7160..7951,7952..8020))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7783..7802
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8021..8125)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8193..8211
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8249..8271)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8273..8293)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8278..8607
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8458..8593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8520..8539
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8744..8763
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8830..9285)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8967..8988)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9179..9198
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9411..9433
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9425..9442
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9426..9442
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc caaacgccac catgccactg
     1621 ccacctctga gctctcggac actgctgctg ctgctcttgc tgctgctgag gggtgtgtgg
     1681 atagccatca gctcgccccc agctggtttg ggtccacaac ctgccttccg cacctttgtg
     1741 gccagtgact ggggcctcac tcacctggtg gttcacgaac agacaggaga ggtatatgtt
     1801 ggcgctgtga accgtattta caagctgtcg gggaacctaa ccctgcttcg ggcccatgtg
     1861 acaggccctg tagaagacaa tgagaaatgc tacccacccc ccagtgtaca gtcctgccca
     1921 catggtctgg gcagcacaga caacgtcaac aagcttctac tgctggacta tgcagccaac
     1981 cgcctgttgg cctgtggcag tgcctcacag ggcatctgcc agttcctgag gttggacgat
     2041 ctcttcaagc tgggcgagcc gcaccaccgc aaggaacact acctgtccag tgttcgagag
     2101 gctggcagca tggcgggagt gctcattgct gggccacccg gccagggcca ggccaagctc
     2161 ttcgtgggca cacccatcga tggcaaatca gaatacttcc ccactctgtc cagtcgccgg
     2221 ctcatggcca acgaggaaga tgctgacatg tttggctttg tgtaccagga tgagtttgtg
     2281 tcatctcagc tcaagatccc ttcagacaca ttgtccaagt tcccagcctt tgacatctac
     2341 tatgtgtaca gtttccgcag cgagcagttc gtatactacc tcaccctgca gctagacacg
     2401 cagcttacct cgcccgacgc cgctggcgag cacttcttca cctccaagat tgtgcggctg
     2461 tgcgtgaatg accccaagtt ctactcttac gtggagttcc ccattggctg cgagcaggct
     2521 ggcgtggagt atcggctggt gcaggacgcc tacctgagcc ggccgggaca ggccctggcc
     2581 aaacagctgg gcctggctga ggatgaggag gtgttgttca ctgtgttcgc acagggccag
     2641 aagaaccgcg tgaagccacc caaggagtcg gcgctgtgcc tgttcacact gagggccatc
     2701 aaggagaaaa tcaaggagcg catccaatcc tgctaccgtg gagaaggaaa gctctccctg
     2761 ccttggctgc tcaacaagga gctgggctgc atcaactcgc ccctgcagat tgatgacgac
     2821 ttctgcgggc aggatttcaa ccagccactg ggtggcacgg tcaccatcga gggcacaccc
     2881 ctttttgtcg acaaggaaga tggcctgact gctgtggctg cctatgacta ccaaggccgc
     2941 accgtggttt ttgctggcac tcgcagcggc cgcatccgca agattctggt ggaccttgca
     3001 aaccccagcg gcaggccggc cctggcttat gaaagtgtgg tggctcagga gggcaaccct
     3061 atcctgcgtg acctggtcct cagccccaac cgccagtacc tctatgctat gacggagaag
     3121 caggtgacgc aggtgcctgt ggaaagctgt gtccagtata cttcctgtga gctgtgcctg
     3181 gggtctcggg acccccactg tggctggtgt gtcctgcaca gcatctgctc taggcaggat
     3241 gcctgtgagc gggcagaaga gccccagcgt tttgcctctg acctgctgca gtgtgtgcag
     3301 ttaactgtcc agccgcgcaa tgtgtcagtc accatgtccc aggtgccgct cgtgctgcag
     3361 gcttggaatg tgcctgacct ctcagctggt gtcaactgct cctttgagga cttcacggag
     3421 actgagagca tcctggaaga tggccgcatc cattgccact caccttctgc ccgggaagtg
     3481 gcgcccatca cacagggtca gggagaccag cgggtggtga agctctacct caagtccaag
     3541 gagacaggga agaagtttgc atctgtggac tttgtcttct ataactgcag tgttcaccag
     3601 tcctgcctgg catgtgtcaa cggctccttc ccctgccatt ggtgcaaata ccgccatgtt
     3661 tgcaccaaca atgcggctga ctgcgccttt ctggaaggcc gagtcaacat gtctgaggac
     3721 tgcccacaga tcctgccttc tacccacatc tatgtgccag tgggtgtggt gaagcccatc
     3781 accctggctg cacggaacct gccgcagcca cagtcgggtc agcgagggta cgagtgcctt
     3841 ttccacatcc caggcagccc ggcccgagtc actgcactgc gcttcaacag ctccagcctg
     3901 cagtgccaga actcctcgta ctcctatgag gggaatgatg tcagcgatct gcctgtgaac
     3961 ctgtcggttg tgtggaatgg caactttgtc attgacaacc cccagaacat ccaggcccac
     4021 ctctacaagt gcccagcctt gcgccagagt tgtggcctct gcctcaaggc agacccacgc
     4081 tttgagtgcg gctggtgcgt agctgagcgc cgctgttccc ttcgtcacca ctgcccagct
     4141 gactcaccag cctcctggat gcatgcccac catggcagca gccgctgcac agaccccaag
     4201 attctcaagc tgtccccaga gacaggccct cggcagggag ggactcgact aaccatcacc
     4261 ggtgagaatc tgggcctccg gtttgaggat gtgcggctag gcgtgcacgt gggcaaggtg
     4321 ctttgcagcc ctgtggagag cgagtacatc agcgcagaac agattgtctg cgagattgga
     4381 gatgccagca cgctgcgggc ccatgatgcc ctggtggagg tgtgtgtgcg ggactgctcc
     4441 ctccactacc gagcgctgtc ccctaaacgc ttcacttttg tgacaccgac cttctaccgt
     4501 gtgagcccct cccgtgggcc tctgtctgga ggtacctgga ttggcatcga agggagccat
     4561 ctgaatgctg gcagtgatgt agctgtgtcc attggcggtc ggccctgctc cttctcctgg
     4621 agaaactcac gagagatccg ctgcctgaca ccgcccgggc acactcctgg cagcgcccct
     4681 attgttatca acatcaatcg tgcccagctc tccaaccccg aggtgaagta caactatact
     4741 gaagacccta ctatcctgag gatcgaccct gagtggagca tcaatagtgg tgggaccctc
     4801 ctaacagtca cgggcaccaa cctggccact gtccgtgaac ctcgaatccg cgccaagtat
     4861 ggaggcattg agagggaaaa tagctgcatg gtgtacaatg acaccaccat ggtgtgccgg
     4921 gcgccgtcta tagacaaccc aaagcgcagc ccgccagaac tgggagagcg gccagatgag
     4981 attggtttca tcatggacaa tgtgcgcacg ctgctggtac tcaacagctc ctccttcctc
     5041 tactacccgg accctgtact ggagccactt agccctactg ggctcttgga gctgaagccc
     5101 agctccccgc tcatcctcaa ggggcggaat ctcctgccac ctgctcctgg caattcccgg
     5161 ctcaactaca cggtgctcat cggctccaca ccttgtatcc tcaccgtgtc tgagacacag
     5221 ctgctgtgtg aagcacccaa cctcacgggg cagcacaaag tcacggtgcg ggccgggggc
     5281 ttcgagttct cacctgggat gctgcaggtg tattcggaca gcctgctgac actgactagt
     5341 gacaaaactc acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc
     5401 ttcctcttcc ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca
     5461 tgcgtggtgg tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac
     5521 ggcgtggagg tgcataatgc caagacaaag ccgcgggagg agcagtacaa cagcacgtac
     5581 cgtgtggtca gcgtcctcac cgtcctgcac caggactggc tgaatggcaa ggagtacaag
     5641 tgcaaggtct ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa
     5701 gggcagcccc gagaaccaca ggtgtatacc ctgcccccat cccgggatga gctgaccaag
     5761 aaccaggtca gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag
     5821 tgggagagca atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc
     5881 gacggctcct tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg
     5941 aacgtcttct catgctccgt gatgcatgag gctctgcaca accactacac gcagaagagc
     6001 ctctccctgt ctccgggtaa aggcggccat catcaccatc accattaatc tagagatctt
     6061 attaaagcag aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6121 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6181 caatgtatct tatcatgtct ggtcgactct agctagactc ttccgcttcc tcgctcactg
     6241 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6301 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6361 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6421 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6481 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6541 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6601 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6661 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6721 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6781 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6841 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6901 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6961 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7021 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7081 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     7141 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     7201 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7261 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     7321 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7381 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7441 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7501 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7561 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7621 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7681 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7741 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7801 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7861 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7921 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7981 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8041 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     8101 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     8161 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     8221 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gaggaatgtg
     8281 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8341 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     8401 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     8461 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     8521 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     8581 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgtc
     8641 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     8701 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     8761 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     8821 cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     8881 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     8941 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     9001 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     9061 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     9121 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     9181 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     9241 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag
     9301 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     9361 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     9421 acgttgtaaa acgacggcca gtgaatt
//
