LOCUS       sequence-43942-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  TITLE     Stainier unpublished
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: bActin-RSG-BiSKi
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    58..91
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    146..280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(170..189)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     224..243
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(705..725)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctcaccatg gtggcgaccg gtggatccgt cgaggggatc gatccggaac ccttaatata
       61 acttcgtata atgtatgcta tacgaagtta ttaggtccct cgacctgcag cccaagctcc
      121 ggggataagc ttacttacca tgtcagatcc agacatgata agatacattg atgagtttgg
      181 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      241 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      301 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
      361 caaatgtggt atggctgatt atgatctcta gtcaaggcac tatacatcaa atattcctta
      421 ttaacccctt tacaaattaa aaagctaaag gtacacaatt tttgagcata gttattaata
      481 gcagacactc tatgcctgtg tggagtaaga aaaaacagta tgttatgatt ataactgtta
      541 tgcctactta taaaggttac agaatatttt tccataattt tcttgtatag cagtgcagct
      601 ttttcctttg tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga
      661 ctcaaaaaac ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt
      721 tttggaggag tagaatgttg agagtcagca gtagcctcat catcactaga tgg
//
