LOCUS       sequence-439422-        9433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinA2 protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxna2-Fc-His
FEATURES             Location/Qualifiers
     source          1..9433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1607..1616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6014..6031
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6057..6178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6094..6113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6148..6167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6216..6233)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6387..6975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6467..6486)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7146..7937,7938..8006))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7769..7788
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8007..8111)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8179..8197
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8235..8257)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8259..8279)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8264..8593
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8444..8579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8506..8525
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8730..8749
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8816..9271)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8953..8974)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9165..9184
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9397..9419
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9411..9428
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9412..9428
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgccgcgcca ccatggagca
     1621 gaggaggttc tatctgcggg ccatgcaggc tgacaacctg tctgtggttc tgctctcagt
     1681 ggcctggctc ctgctagctc gtgggaccac aggtatgcct cagtacagca ctttccactc
     1741 tgagaatcgt gactggactt tcaaccattt gactgtacac cgaagaacag gggctgtgta
     1801 tgtgggggct atcaatcgtg tctacaagtt gactggcaac ctcaccatcc aggtggctca
     1861 caagacaggg ccagaagagg acaacaaggc ttgctaccca cccctcattg tacagccctg
     1921 cagtgaagtg cttacactca ccaacaatgt caacaaacta ctgatcattg actactctga
     1981 gaatcgcctg ctggcctgcg gaagcctcta ccagggtgtt tgcaagctcc tgcgactaga
     2041 tgacctcttc atcctggtgg agccatccca caagaaggaa cactacttgt ccagtgtcaa
     2101 taagacaggc accatgtatg gtgtgattgt gcgctctgag ggggaagatg gcaagctttt
     2161 tatcggcact gctgtggatg gcaagcagga ttacttccct actctgtcca gccgcaagct
     2221 gccccgtgac cctgagtctt cagcaatgct ggactatgag ctccacagtg attttgtctc
     2281 ctccctcatc aagattccct ctgacaccct agccctggtc tctcacttcg acatcttcta
     2341 catctatggc tttgccagtg gggggtttgt ctactttctc actgtccagc cagagacccc
     2401 tgacggcatg gccatcaatt cagctggaga cctcttctat acctcaagaa ttgtgcgtct
     2461 ctgcaaggat gaccccaagt tccactccta tgtgtccctg ccttttggct gcacacgtgc
     2521 tggggtggaa tatcgccttc tgcaggcagc ttaccttgca aagccagggg aagctctagc
     2581 tcaggccttc aacatcagca gcgacgaaga tgtcctgttt gccatcttct ccaaggggca
     2641 gaagcagtac caccaccccc ctgatgactc tgccctctgt gccttcccca tccgggccat
     2701 caacttgcaa atcaaggagc ggttgcagtc ctgctaccat ggagagggca acttggagct
     2761 caactggctg ctgggaaagg atgtgcagtg caccaaggcg cctgtcccaa tcgatgataa
     2821 cttctgcggc ctggacatca accagcctct gggaggctcc actcctgtgg agggactgac
     2881 cctgtatacc accagcaggg accgcctgac ctctgtggcc tcctatgttt acaatggcta
     2941 cagtgtggtt tttgtgggga ctaagagtgg caagctgaag aagattcgag ctgatggtcc
     3001 cccccatggt ggggtccagt atgagatggt ctctgtgttc aaagatggga gcccaatcct
     3061 ccgggacatg gccttctcca tcaatcagct atacctatat gtcatgtctg agagacaggt
     3121 caccagggtc cctgttgaat catgtgaaca gtatacaact tgtggagagt gtctaagctc
     3181 aggggatcct cactgtggct ggtgtgccct gcacaacatg tgctcccgaa gagacaaatg
     3241 ccaacgggcc tgggaagcaa atcgatttgc tgccagtatc agccagtgca tgagccttga
     3301 ggtacacccc aacagcatct ctgtgtcaga tcacagccgg ctgctcagcc tggttgtgaa
     3361 tgatgctccc aacctctctg aaggtattgc ttgtgccttt gggaatctga ctgaggtgga
     3421 gggacaggta tctgggagtc aagtcatctg catctcacct ggacccaagg atgtccctgt
     3481 catccctctg gatcaagact ggtttggcct agagctgcag ctgagatcca aagagacagg
     3541 aaagatcttt gtcagcaccg aattcaagtt ctataactgc agtgcccacc aactgtgcct
     3601 gtcctgtgtt aacagcgcct tccgctgcca ttggtgcaag taccgtaacc tctgcacaca
     3661 tgaccccact acctgttcct tccaggaagg caggatcaat gtttcagagg actgtcccca
     3721 gctcgtgccc acggaggaga ttctgatccc agttggggaa gtaaaaccaa tcacccttaa
     3781 ggcccgaaac ctgccccagc cccagtctgg ccagcgaggc tacgagtgtg tgctcagcat
     3841 tcaaggggct gtccaccggg tccctgccct gcgtttcaac agttccagtg tgcagtgcca
     3901 aaacagctcg taccagtatg atgggatgga catcagcaac ctagcagtgg actttgctgt
     3961 agtatggaat ggcaacttca ttattgacaa ccctcaggac ctgaaagtac atctctacaa
     4021 gtgtgcagcc cagcgggaaa gctgtggtct ctgcctcaag gctgaccaca agttcgagtg
     4081 tggctggtgc agtggtgagc gcagatgtac cctccaccag cactgcccca gcacttctag
     4141 cccctggctt gactggtcca gccacaatgt caagtgttcc aacccccaaa tcacagagat
     4201 tttgacagta tcaggaccac ctgaaggagg gactcgtgtg accatccatg gcgtgaacct
     4261 gggcttggac ttctctgaga ttgctcacca tgtgcaggtg gctggagtgc cctgcacacc
     4321 tatcccaggg gaatacatca tcgctgagca gatcgtctgt gagatgggcc atgccgttat
     4381 aggtaccaca tctgggcctg tgcgcctgtg cattggggaa tgcaagccag agttcatgac
     4441 caagtcccac cagcagtata cttttgtgaa tccttctgtg ctgtcactca gcccgatccg
     4501 gggaccagag tcaggaggta ccatggtgac catcacaggc cattaccttg gtgctgggag
     4561 cagtgtggca gtgtacctgg gcaatcagac ctgtgaattc tatgggaggt caatgaatga
     4621 gattgtatgt gtttcacccc catcatccaa tggactagga ccagtccctg tctccgtgag
     4681 tgtcgacaga gcccgggtgg atagcagtct gcagttcgag tatatagatg acccacgggt
     4741 ccaacgtatt gagccagagt ggagtatcac tagtgggcac acacccctaa ccatcacagg
     4801 cttcaacttg gatgtcattc aggagcccag ggtccgagtc aaatttaatg gcaaagaatc
     4861 tgtcaatgta tgcacagtgg taaacacaac caccctcacc tgtctggcac cctctctgac
     4921 cagtgactac cgcccaggtc tggacactgt ggaacggcca gatgagtttg gatttctctt
     4981 taacaatgtt caatccttac tcatctataa cgacaccaag ttcatctact accccaaccc
     5041 aacgtttgaa ctgctcagcc ccactggaat cttggatcag aagccaggct cacccatcat
     5101 cctgaagggc aaaaatctct gtcctcctgc ctctggaggg gccaaactca actacacagt
     5161 aatgattgga gagacacctt gtacagtcac tgtgtctgag acacagctgc tttgtgaacc
     5221 tcccaacctc acagggcagc acaaggtcat ggttcacgtg ggcgggatgg tgttctcacc
     5281 tggctccgtg agcgtcatct ccgacagcct gttgaccctg tctagtgaca aaactcacac
     5341 atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc
     5401 aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga
     5461 cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca
     5521 taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt
     5581 cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa
     5641 caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga
     5701 accacaggtg tataccctgc ccccatcccg ggatgagctg accaagaacc aggtcagcct
     5761 gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg
     5821 gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt
     5881 cctctacagc aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg
     5941 ctccgtgatg catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc
     6001 gggtaaaggc ggccatcatc accatcacca ttaatctaga gatcttatta aagcagaact
     6061 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6121 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6181 atgtctggtc gactctagct agactcttcc gcttcctcgc tcactgactc gctgcgctcg
     6241 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6301 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6361 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6421 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6481 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6541 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6601 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6661 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6721 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6781 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6841 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6901 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6961 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7021 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7081 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7141 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7201 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7261 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7321 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7381 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7441 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7501 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7561 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7621 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7681 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7741 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7801 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7861 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7921 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7981 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8041 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8101 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     8161 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     8221 gatgacggtg aaaacctctg acacatgcag ctcccggagg aatgtgtgtc agttagggtg
     8281 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8341 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8401 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     8461 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8521 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8581 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcgtcccgg agacggtcac
     8641 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8701 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8761 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     8821 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     8881 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     8941 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     9001 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     9061 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     9121 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     9181 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     9241 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     9301 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     9361 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     9421 cggccagtga att
//
