LOCUS       sequence-439431-        9436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            ZNF281.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9436)
  AUTHORS   Pierson Smela M, Kramme CC, Fortuna PRJ, Wolf B, Srikar Kavirayuni 
            V, Adams J, Ma C, Velychko S, Widocki U, Goel S, Chen T, Vincoff S, 
            Dong E, Kohman RE, Kobayashi M, Shioda T, Church GM, Chatterjee P
  TITLE     Rapid Human Oogonia-like Cell Specification via Combinatorial 
            Transcription Factor-Directed Differentiation
  JOURNAL   bioRxiv 2022.07.11.499564
REFERENCE   2  (bases 1 to 9436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.11.499564"
COMMENT     Sequence Label: pPB-cT3G-cERH-ZNF281
FEATURES             Location/Qualifiers
     source          1..9436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     116..133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    339..360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(375..380,381..398,399..405)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    413..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     418..440
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     437..453
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     437..453
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   479..541
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    717..798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(915..1940)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1941..1997)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2007..2750)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(2751..2774)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2774..2783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2803..3014)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        3015..3394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    3603..3621
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3639..3657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3675..3693
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3711..3729
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3747..3765
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3783..3801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3819..3837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3864..3888
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3954..3978
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4398..4421
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     protein_bind    complement(6664..6688)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6690..6716
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6730..6864
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7136..7170
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7181..7198)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7181..7197)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7190..7212)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7406..7425)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7527..7549
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7588..7606)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7674..7778
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7779..7847,7848..8639)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7997..8016)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8810..9398
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9299..9318
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
       61 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      121 gtcagtgagc gaggaagcgg atgagcgccc aatacgcaaa ccgcctctca ccgcgcgttg
      181 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      241 caacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      301 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      361 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      421 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaaggt cgacttaacc
      481 ctagaaagat aatcatattg tgacgtacgt taaagataat catgcgtaaa attgacgcat
      541 gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata ttaagtttta
      601 ttatatttac acttacatac taataataaa ttcaacaaac aatttattta tgtttattta
      661 tttattaaaa aaaaacaaaa actcaaaatt tcttctataa agtaacaaaa cttttagcag
      721 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      781 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
      841 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
      901 gatcctctgg agatctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta
      961 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
     1021 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa
     1081 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg
     1141 agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag
     1201 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg
     1261 tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg
     1321 tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag
     1381 tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg
     1441 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc
     1501 cacgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta
     1561 agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt
     1621 tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc
     1681 aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca
     1741 aagtgccgat gaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg
     1801 acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc
     1861 tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg
     1921 gtgagttcag gctttttcat ggggccgggg ttctcctcca cgtcaccggc ctgcttcagc
     1981 aggctgaagt tggtggcgcc gctgcccccg gggagcatgt caaggtcaaa atcgtcaaga
     2041 gcgtcagcag gcagcatatc aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct
     2101 aagtcaaaat cgtcaagggc gtcggtcggc ccgccgcttt cgcactttag ctgtttctcc
     2161 aggccacata tgattagttc caggccgaaa aggaaggcag gttcggctcc ctgccggtcg
     2221 aacagctcaa ttgcttgttt cagaagtggg ggcatagaat cggtggtagg tgtctctctt
     2281 tcctcttttg ctacttgatg ctcctgttcc tccaatacgc agcccagtgt aaagtggccc
     2341 acggcggaca gagcgtacag tgcgttctcc agggagaagc cttgctgaca caggaacgcg
     2401 agctgatttt ccagggtttc gtactgtttc tctgttgggc gggtgccgag atgcacttta
     2461 gccccgtcac gatgtgagag gagagcacag cggtatgact tggcgttgtt ccgcagaaag
     2521 tcttgccatg actcgccttc cagggggcag gagtgggtat gatgcctgtc cagcatctcg
     2581 attggcaggg catcgagcag ggcccgcttg ttcttcacgt gccagtacag ggtaggctgc
     2641 tcaactccca gcttttgagc gagtttcctt gtcgtcaggc cttcgatacc gactccattg
     2701 agtaattcca gagcagagtt tatgactttg ctcttgtcca gtctagacat cttatcgtca
     2761 tcgtctttgt aatccatggt ggcggatccc gcgtcacgac acctgtgttc tggcggcaaa
     2821 cccgttgcga aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc
     2881 ctcgggaaaa aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct
     2941 tctctaggca ccggttcaat tgccgacccc tccccccaac ttctcgggca ctgtgggcca
     3001 tgtgcgctct gccccatggt aatagccatg actaatacgt agatgtactg ccaagtagga
     3061 aagtgccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     3121 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     3181 cgtaaatact ccacccattg acgtcaatgg aaagtgccta ttggcgttac tatgggaaca
     3241 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     3301 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     3361 gattactatt aataactagt caataatcaa tgtcataagc tctaatcgca taatcgagct
     3421 aagagcgatt aatatgctta gctagcttag ctaagctaga tttctctatt ctcccacaat
     3481 atatagcagc tattagctta gcagtccgaa tacgatcgtc cacgatccat aagcgtaaca
     3541 catttgagta gcgactgacg ggcaccggag ccactcgagt ggaattatca cctcgagttt
     3601 actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg
     3661 tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag
     3721 tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt
     3781 actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg
     3841 tataagcttg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     3901 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct accacaagtt
     3961 tgtacaaaaa agttggcatg aaaatcggca gtgggttcct gagtggcggc ggaggtaccg
     4021 gcagtagcgg tggtagcggc tccggcggcg gtggtagtgg cggcggcggc ggcggcggca
     4081 gcagcggcag gagggcagag atggaaccca cctttcccca gggtatggtt atgttcaacc
     4141 accgtcttcc cccggtcacc agcttcaccc ggccggcggg gtcggccgcc cctcccccgc
     4201 aatgcgtgtt atcctcctct acctccgcag ccccggccgc tgagcccccc cctccgccag
     4261 ccccggacat gactttcaag aaggagccgg cggcgtcagc cgcggccttc ccctcgcaga
     4321 ggacctcctg ggggttcttg cagtctttgg ttagcatcaa acaggagaaa cccgcggatc
     4381 ctgaggagca gcagtcccac caccaccatc accaccacca ctatgggggg ctgttcgctg
     4441 gagctgaaga gaggtctcca ggcctaggag gcggtgaagg ggggagtcac ggcgtcatcc
     4501 aggacctcag tattctccac cagcatgtcc agcagcaacc agcccagcac caccgtgacg
     4561 tattactcag cagcagtagc aggactgatg accaccatgg cactgaggag ccaaagcagg
     4621 acactaatgt caaaaaggca aaaaggccaa agccagaatc tcagggaatc aaagccaaga
     4681 ggaagccaag tgcatcttcc aaaccttctt tggttggaga tggagaaggt gccatcctct
     4741 ccccaagtca gaaacctcat atctgtgatc actgtagtgc tgctttccga agctcctatc
     4801 acctgcggag acatgtcctc attcatacag gagaaagacc tttccagtgc agccagtgta
     4861 gtatgggttt cattcagaaa tacctactac agagacatga gaaaattcat agtagagaga
     4921 agccatttgg atgtgatcag tgcagcatga agtttattca gaagtaccat atggagagac
     4981 acaagaggac acatagtgga gaaaagccat ataagtgtga cacttgccaa cagtattttt
     5041 caaggactga tagattgttg aagcacaggc gcacatgtgg tgaagtcata gttaaaggag
     5101 ccactagtgc agaacctggg tcatcaaacc ataccaatat gggtaatctg gctgtgttgt
     5161 ctcagggaaa tacaagttct tcaaggagaa aaacaaagtc aaaaagcata gctattgaaa
     5221 ataaggaaca gaagaccggt aaaacaaatg aatcacaaat ttcaaataat ataaacatgc
     5281 agagttactc agtagaaatg cctaccgtgt cttccagtgg aggcataatt ggcactggaa
     5341 tagatgaact gcagaagagg gtgccaaaat tgatctttaa gaaaggaagc agaaagaata
     5401 cagataaaaa ctaccttaac tttgtgtcac cattaccaga catagtagga cagaaatcct
     5461 tgtctggaaa accaagtggc tcacttggca tagtatcaaa taatagtgtg gagaccattg
     5521 gtcttctcca aagtacaagt ggcaaacaag gtcagataag tagtaattat gatgatgcca
     5581 tgcagttttc aaagaaaaga agatatttac caactgccag cagcaacagt gccttttcta
     5641 taaacgtagg acacatggtc tcccaacagt ctgtcattca gtctgcaggt gtcagtgttt
     5701 tggacaatga ggcaccattg tcacttattg actcctcagc tctaaatgct gaaattaaat
     5761 cttgtcatga caagtctgga attcctgatg aggttttaca aagtattttg gatcaatact
     5821 ccaacaaatc agaaagccag aaagaggatc ctttcaatat tgcagaacca cgagtggatt
     5881 tacacacctc aggagaacac tcagaattgg ttcaagaaga aaatttgagc ccaggcaccc
     5941 aaacaccttc aaatgataaa gcaagtatgt tgcaagaata ctccaaatac ctccaacagg
     6001 cttttgaaaa atccactaat gcaagtttta ctcttggaca cggtttccaa tttgtcagtt
     6061 tgtcttcacc tctccacaac cacactttgt ttccagaaaa acaaatatac actacgtcac
     6121 ctttggagtg tggtttcggc caatctgtta cctcagtgtt gccatcttca ttgccaaagc
     6181 ctccttttgg gatgttgttt ggatctcagc caggtcttta tttgtctgct ttggatgcta
     6241 cacatcagca gttgacacct tcccaggagc tggatgatct gatagattct cagaagaact
     6301 tagagacttc atcagccttc cagtcctcat ctcagaaatt gactagccag aaggaacaga
     6361 aaaacttaga gtcttcaaca ggctttcaga ttccatctca ggagttagct agccagatag
     6421 atcctcagaa agacatagag cctagaacaa cgtatcagat tgagaacttt gcacaagcgt
     6481 ttggttctca gtttaagtcg ggcagcaggg tgccaatgac ctttatcact aactctaatg
     6541 gagaagtgga ccatagagta aggacttcag tgtcagattt ctcagggtat acaaatatga
     6601 tgtctgatgt aagtgagcca tgtagtacaa gagtaaagac acccaccagc cagagttaca
     6661 ggtacccaac tttcttgtac aaagtggtat acccatacga tgttccagat tacgcttaac
     6721 tttctccgcg atccagacat gataagatac attgatgagt ttggacaaac caaaactaga
     6781 atgcagtgaa aaaaatgcct tatttgtgaa atttgtgatg ctattgcctt atttgtaacc
     6841 attataagct gcaataaaca agttatctat aacaagaaaa tatatatata ataagttatc
     6901 acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac
     6961 gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac
     7021 acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa
     7081 atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc
     7141 gtcaatttta cgcagactat ctttctaggg ttaagaattc actggccgtc gttttacaac
     7201 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7261 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7321 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     7381 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     7441 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     7501 cttacagaca agctgtgacc atgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7561 atcaccgaaa cacgcgagtt cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7621 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7681 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7741 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7801 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7861 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7921 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7981 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8041 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8101 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8161 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8221 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8281 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8341 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8401 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8461 ttattgctga taaatctgga gccggtgagc gtggttcacg cggtatcatt gcagcactgg
     8521 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8581 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8641 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8701 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8761 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8821 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8881 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8941 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9001 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9061 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9121 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9181 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9241 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9301 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9361 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9421 tacggttcct ggcctt
//
