LOCUS       sequence-439433-        8780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homology directed repair template for knocking in mVenus reporter to
            NANOS3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Pierson Smela M, Kramme CC, Fortuna PRJ, Wolf B, Srikar Kavirayuni 
            V, Adams J, Ma C, Velychko S, Widocki U, Goel S, Chen T, Vincoff S, 
            Dong E, Kohman RE, Kobayashi M, Shioda T, Church GM, Chatterjee P
  TITLE     Rapid Human Oogonia-like Cell Specification via Combinatorial 
            Transcription Factor-Directed Differentiation
  JOURNAL   bioRxiv 2022.07.11.499564
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.11.499564"
COMMENT     Sequence Label: pHDR_NANOS3-T2A-mVenus-PuroTK
FEATURES             Location/Qualifiers
     source          1..8780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             303..875
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          952..1017
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     rep_origin      complement(1300..1888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1380..1399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2059..2850,2851..2919))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2682..2701
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2920..3024)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3050..3505)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3187..3208)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3399..3418
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3632..3654
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3646..3663
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3647..3663
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3673..3692
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3673..3691
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4632..4685
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4686..4688,4689..4691,4692..5405)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4731..4752)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4992..5011)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    5406..5437
                     /label=rox
                     /bound_moiety="Dre recombinase"
     promoter        5487..5986
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5522..5545)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5919..5938
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      6000..6009
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6006..6605
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6505..6525
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6612..7607
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(7629..7646)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7635..7859
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7907..7938
                     /label=rox
                     /bound_moiety="Dre recombinase"
ORIGIN
        1 acctccagat cttgtcagtg tggttttcaa gaggaagcaa aaagcctctc cacccaggcc
       61 tggaatgttt ccacccaatg tcgagcagtg tggttttgca agaggaagca aaaagcctct
      121 ccacccaggc ctggaatgtt tccacccaat gtcgagcaaa ccccgcccag cgtcttgtca
      181 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat
      241 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcaggtcctc gccatggatc
      301 ctgatgatgt tgttgattct tctaaatctt ttgtgatgga aaacttttct tcgtaccacg
      361 ggactaaacc tggttatgta gattccattc aaaaaggtat acaaaagcca aaatctggta
      421 cacaaggaaa ttatgacgat gattggaaag ggttttatag taccgacaat aaatacgacg
      481 ctgcgggata ctctgtagat aatgaaaacc cgctctctgg aaaagctgga ggcgtggtca
      541 aagtgacgta tccaggactg acgaaggttc tcgcactaaa agtggataat gccgaaacta
      601 ttaagaaaga gttaggttta agtctcactg aaccgttgat ggagcaagtc ggaacggaag
      661 agtttatcaa aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt cccttcgctg
      721 aggggagttc tagcgttgaa tatattaata actgggaaca ggcgaaagcg ttaagcgtag
      781 aacttgagat taattttgaa acccgtggaa aacgtggcca agatgcgatg tatgagtata
      841 tggctcaagc ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga accttacttc
      901 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
      961 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     1021 ccaacctatg gaactgatga atgggagcag tggtggaatg cagatccact agagtcgagg
     1081 gatgcagaaa ttgatgatct attaaacaat aaagatgtcc actaaaatgg aagtttttcc
     1141 tgtcatactt tgttaagaag ggtgagaaca gagtacctac attttgaatg gaaggattgg
     1201 agctacgggg gtgggggtgg ggtgggatta gataaatgcc tgctctttac tgaaggctct
     1261 ttactattgc tttatgataa tgtttcatag ttggatgttt ttccataggc tccgcccccc
     1321 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1381 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1441 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1501 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1561 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1621 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1681 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1741 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1801 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1861 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1921 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1981 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2041 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2101 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2161 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2221 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2281 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     2341 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     2401 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     2461 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2521 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2581 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2641 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2701 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2761 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2821 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2881 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2941 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3001 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     3061 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     3121 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     3181 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     3241 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     3301 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     3361 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     3421 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     3481 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     3541 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     3601 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     3661 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag
     3721 gtcgacgtgc ctgtagtccc agctacttgg gaggctgacg agggaggatc gcttgggtcc
     3781 aggggacaga ggctgcagtg agctgagatg gtgccactgc actgcagcct gggcaacaca
     3841 gcaagacctc gtcttaaaaa aaaaaaaaag cactggtcat ggtggctcat gcctgtaatc
     3901 ccagtacttg gggaagctga ggcaggtgga tcacctgagg tcaggaattc aagaccagcc
     3961 tggccaacat ggtgaaaccc cgtctctact gaaaatacaa aaaattaact gggcatggtg
     4021 gcatgcgcct gtaatccctg ctactcgggg ggctgagaca ggagaatcgc tagaacccgg
     4081 gaggcggagg tcgcagtgag ctgaggtcac gtcactgcac tccagcctgg gcgacagagt
     4141 gagactccgt ctcacaaaaa aaagaaagca tgaaagactg cctccaggag aagatagtgt
     4201 ttcctgtgag gtttgatgct gtctcactgg gagacgctgg aggccaaaag gacataggga
     4261 agatggtgtc ctgcggccag cctttctgat gaacaacttt tgggagtaac agatccgggg
     4321 accccagagc acggcggcca cgagagggca ccacagtcgc agaatcggaa ctcctgtgct
     4381 ttgtctcggg ctctgggtcc cagcggggcc gcccggcgga gccagagacg ccacggggtc
     4441 gctgtctccg gagcagcccc tgggtggcgc cagaggtcca gcaggcccgg aggccgagtc
     4501 cgtgggcaac ttggggagcg ttgagtcatc gcctgtgatt aagcatttct ctccctcccc
     4561 ctccactagg tttcagaggt gccgggaagt ctgagccttc gccctcctgc tctccctcca
     4621 tgtccaccta cgagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
     4681 gcccaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     4741 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     4801 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc
     4861 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
     4921 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     4981 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     5041 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     5101 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
     5161 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc
     5221 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     5281 accactacct gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     5341 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     5401 agtaataact ttaaataatg ccaattattt aaagttacat atgagaaagt ataggaactt
     5461 cagcttgata tcgaattaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     5521 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     5581 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     5641 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     5701 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     5761 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     5821 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
     5881 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     5941 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagccaacg
     6001 ccaccatggg gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc
     6061 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6121 acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6181 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6241 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6301 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6361 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6421 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6481 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6541 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6601 gtgccggatc catgcccacg ctactgcggg tttatataga cggtcctcac gggatgggga
     6661 aaaccaccac cacgcaactg ctggtggccc tgggttcgcg cgacgatatc gtctacgtac
     6721 ccgagccgat gacttactgg caggtgctgg gggcttccga gacaatcgcg aacatctaca
     6781 ccacacaaca ccgcctcgac cagggtgaga tatcggccgg ggacgcggcg gtggtaatga
     6841 caagcgccca gataacaatg ggcatgcctt atgccgtgac cgacgccgtt ctggctcctc
     6901 atatcggggg ggaggctggg agctcacatg ccccgccccc ggccctcacc ctcatcttcg
     6961 accgccatcc catcgccgcc ctcctgtgct acccggccgc gcgatacctt atgggcagca
     7021 tgacccccca ggccgtgctg gcgttcgtgg ccctcatccc gccgaccttg cccggcacaa
     7081 acatcgtgtt gggggccctt ccggaggaca gacacatcga ccgcctggcc aaacgccagc
     7141 gccccggcga gcggcttgac ctggctatgc tggccgcgat tcgccgcgtt tacgggctgc
     7201 ttgccaatac ggtgcggtat ctgcagggcg gcgggtcgtg gcgggaggat tggggacagc
     7261 tttcggggac ggccgtgccg ccccagggtg ccgagcccca gagcaacgcg ggcccacgac
     7321 cccatatcgg ggacacgtta tttaccctgt ttcgggcccc cgagttgctg gcccccaacg
     7381 gcgacctgta caacgtgttt gcctgggcct tggacgtctt ggccaaacgc ctccgtccca
     7441 tgcacgtctt tatcctggat tacgaccaat cgcccgccgg ctgccgggac gccctgctgc
     7501 aacttacctc cgggatggtc cagacccacg tcaccacccc cggctccata ccgacgatct
     7561 gcgacctggc gcgcacgttt gcccgggaga tgggggaggc taactgagct ctagagctcg
     7621 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     7681 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     7741 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     7801 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     7861 ttctgaggcg gaaagaacca gctggggctc gacatactag agtacttaac tttaaataat
     7921 gccaattatt taaagttagg cgcgccgata tctagaccca gctgaggctg cctacacctg
     7981 ggcaagggca ccggggctcg gctggatttc caggaagacc caccctatga cgactgcccc
     8041 cactcccaga ccctcccccc agcctgggat tgagccctgg ggatgacccg gggttggcaa
     8101 gggaagagct gaaatcgccc tgtccttggg gaccccaccc tttgtgccat ctgccgtttc
     8161 cctagtgctg ggcatccagt cagccctcaa taaatgctta tgaatggatc aacaacagac
     8221 aagccctggt cttgggacaa ggtgtagtgt gaaccttgct gggagtcctg tacctagatc
     8281 tccccttctc cttctttgag tttcggcacc ctggggtgct ggggtgagct gctttgtgca
     8341 gagaaacaca agtgggtgcc ttggggtagc tgggggggtt catggcacct caaagccaga
     8401 tcgacatcat gagacccttt ctgtggacat ttggtctttc agccaccccc tgcactgaca
     8461 tgcaagatgg gtggactcct cccatcccac ccccagctcc gtactggagg tgggggcttc
     8521 atttgaggaa gaaattgaca cacaactaga cagagccccc cccccgacat cctcatcact
     8581 aaggaaggtg aatgtttatg tcaccatgga tgccccagtg acaagccagg cagtttagag
     8641 caagggaggg acatttaggg ggcagggaat gggaccatgt cctgggtggg cttgggcagg
     8701 agcagaagga tggggctgtt ctggcagggc gggggggaaa tgcatgtggg ggaatcccca
     8761 cacccctgcc tgcctcctcc
//
