LOCUS       sequence-439434-        7801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            LHX8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7801)
  AUTHORS   Pierson Smela M, Kramme CC, Fortuna PRJ, Wolf B, Srikar Kavirayuni 
            V, Adams J, Ma C, Velychko S, Widocki U, Goel S, Chen T, Vincoff S, 
            Dong E, Kohman RE, Kobayashi M, Shioda T, Church GM, Chatterjee P
  TITLE     Rapid Human Oogonia-like Cell Specification via Combinatorial 
            Transcription Factor-Directed Differentiation
  JOURNAL   bioRxiv 2022.07.11.499564
REFERENCE   2  (bases 1 to 7801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.11.499564"
COMMENT     Sequence Label: pPB-cT3G-cERH-LHX8
FEATURES             Location/Qualifiers
     source          1..7801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6..28
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     25..41
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     25..41
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   67..129
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    305..386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(503..1528)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVHRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1529..1585)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(1595..2338)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(2339..2362)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2362..2371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2391..2602)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        2603..2982
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    3191..3209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3227..3245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3263..3281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3299..3317
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3335..3353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3371..3389
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3407..3425
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3452..3476
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3542..3566
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4617..4641)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4643..4669
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4683..4817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5089..5123
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5134..5151)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5134..5150)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5143..5165)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5359..5378)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5480..5502
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5541..5559)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5627..5731
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5732..5800,5801..6592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5950..5969)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6763..7351
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7252..7271
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7505..7522
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7728..7749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7764..7769,7770..7787,7788..7794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaaggtcg
       61 acttaaccct agaaagataa tcatattgtg acgtacgtta aagataatca tgcgtaaaat
      121 tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt
      181 aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg
      241 tttatttatt tattaaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact
      301 tttagcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      361 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
      421 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
      481 ctgattatga tcctctggag atctattcct ttgccctcgg acgagtgctg gggcgtcggt
      541 ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg
      601 cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct
      661 gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac
      721 caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct
      781 cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg
      841 cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc
      901 ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt
      961 cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga
     1021 cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga
     1081 aatcacgcca cgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg
     1141 tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag
     1201 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
     1261 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
     1321 ccgatgcaaa gtgccgatga acataacgat ctttgtagaa accatcggcg cagctattta
     1381 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
     1441 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     1501 acgtcgcggt gagttcaggc tttttcatgg ggccggggtt ctcctccacg tcgccggcct
     1561 gcttcagcag gctgaagttg gtggcgccgc tgcccccggg gagcatgtca aggtcaaaat
     1621 cgtcaagagc gtcagcaggc agcatatcaa ggtcaaagtc gtcaagggca tcggctggga
     1681 gcatgtctaa gtcaaaatcg tcaagggcgt cggtcggccc gccgctttcg cactttagct
     1741 gtttctccag gccacatatg attagttcca ggccgaaaag gaaggcaggt tcggctccct
     1801 gccggtcgaa cagctcaatt gcttgtttca gaagtggggg catagaatcg gtggtaggtg
     1861 tctctctttc ctcttttgct acttgatgct cctgttcctc caatacgcag cccagtgtaa
     1921 agtggcccac ggcggacaga gcgtacagtg cgttctccag ggagaagcct tgctgacaca
     1981 ggaacgcgag ctgattttcc agggtttcgt actgtttctc tgttgggcgg gtgccgagat
     2041 gcactttagc cccgtcacga tgtgagagga gagcacagcg gtatgacttg gcgttgttcc
     2101 gcagaaagtc ttgccatgac tcgccttcca gggggcagga gtgggtatga tgcctgtcca
     2161 gcatctcgat tggcagggca tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg
     2221 taggctgctc aactcccagc ttttgagcga gtttccttgt cgtcaggcct tcgataccga
     2281 ctccattgag taattccaga gcagagttta tgactttgct cttgtccagt ctagacatct
     2341 tatcgtcatc gtctttgtaa tccatggtgg cggatcccgc gtcacgacac ctgtgttctg
     2401 gcggcaaacc cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct
     2461 cccccaccct cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg
     2521 cgccaccttc tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcgggcact
     2581 gtgggccatg tgcgctctgc cccatggtaa tagccatgac taatacgtag atgtactgcc
     2641 aagtaggaaa gtgccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     2701 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     2761 cagtttaccg taaatactcc acccattgac gtcaatggaa agtgcctatt ggcgttacta
     2821 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     2881 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     2941 ccgtaattga ttactattaa taactagtca ataatcaatg tcataagctc taatcgcata
     3001 atcgagctaa gagcgattaa tatgcttagc tagcttagct aagctagatt tctctattct
     3061 cccacaatat atagcagcta ttagcttagc agtccgaata cgatcgtcca cgatccataa
     3121 gcgtaacaca tttgagtagc gactgacggg caccggagcc actcgagtgg aattatcacc
     3181 tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc tatcagtgat
     3241 agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag gagtttactc
     3301 cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag agaacgtatc
     3361 tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc tatcagtgat
     3421 agagaacgta taagcttgcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     3481 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctac
     3541 cacaagtttg tacaaaaaag caggcttagc caccatgtca gaggagtgcg ggcggactac
     3601 agccctggcg gccgggagga ctcgcaaagg cgccggggaa gagggactgg tgagccccga
     3661 gggagcgggg gacgaggact cgtgctcctc ctcggccccg ctgtccccgt cgtcctcgcc
     3721 ccggtccatg gcctcgggct ccggctgccc tcctggcaag tgtgtgtgca acagttgcgg
     3781 cctggagatc gtggacaaat accttctcaa ggtgaatgac ctatgctggc atgtccggtg
     3841 tctctcctgc agtgtttgca gaacctccct aggaaggcac accagctgtt atattaaaga
     3901 caaagacatt ttctgcaaac ttgattattt cagaaggtat ggaactcgct gctctcgatg
     3961 tgggagacac atccattcta ctgactgggt ccggagagcc aaggggaatg tctatcactt
     4021 ggcatgcttt gcctgctttt cctgcaaaag gcaactttcc acaggagagg agtttgcttt
     4081 ggtggaagag aaagtcctct gcagagtaca ttatgactgc atgctggata atttaaaaag
     4141 agaagtagaa aatgggaatg ggattagtgt ggaaggtgcc ctcctcacag agcaagatgt
     4201 taaccatcca aaaccagcaa aaagagctcg gaccagcttt acagcagatc agcttcaggt
     4261 tatgcaagca caatttgctc aggacaacaa cccagatgca cagacactcc agaaattggc
     4321 agaaaggaca ggcttgagca gacgtgtgat acaggtgtgg tttcagaatt gtagagcacg
     4381 ccacaagaaa cacgtcagtc ctaatcactc atcctccacc ccagtcacag cagccccacc
     4441 ctccaggctg tctccaccca tgttagaaga aatggcttat tctgcctacg tgccccaaga
     4501 tggaacgatg ttaactgcgc tgcatagtta tatggatgct cattcaccaa caactcttgg
     4561 actccagccc ttgttacccc attcaatgac acaactgcca ataagtcata cctaataccc
     4621 agctttcttg tacaaagtgg tatacccata cgatgttcca gattacgctt aactttctcc
     4681 gcgatccaga catgataaga tacattgatg agtttggaca aaccaaaact agaatgcagt
     4741 gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc cttatttgta accattataa
     4801 gctgcaataa acaagttatc tataacaaga aaatatatat ataataagtt atcacgtaag
     4861 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     4921 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     4981 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     5041 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     5101 ttacgcagac tatctttcta gggttaagaa ttcactggcc gtcgttttac aacgtcgtga
     5161 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     5221 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5281 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     5341 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5401 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5461 acaagctgtg accatgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5521 aaacacgcga gttcgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5581 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5641 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5701 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5761 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5821 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5881 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5941 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     6001 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6061 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6121 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6181 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6241 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6301 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6361 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6421 tgataaatct ggagccggtg agcgtggttc acgcggtatc attgcagcac tggggccaga
     6481 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6541 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6601 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6661 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6721 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6781 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6841 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6901 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6961 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7021 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7081 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7141 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7201 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7261 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7321 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7381 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7441 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7501 gcgcagcgag tcagtgagcg aggaagcgga tgagcgccca atacgcaaac cgcctctcac
     7561 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     7621 cagtgagcgc aacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7681 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7741 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7801 a
//
