LOCUS       sequence-43944-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB0c-RFP
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             144..155
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(164..198)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     promoter        237..339
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(406..425)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agtttagcgg tctgggtacc ttcgtacgga cgaccttcac cttcaccttc gatttcgaac
       61 tcgtgaccgt taacggaacc ttccatacga actttgaaac gcatgaactc tttgataacg
      121 tcttcggagg aagccatcta gtatttctcc tctttcacta gtagctagca cagtacctag
      181 gactgagcta gctgtcaaag atctcatgaa ttcgatatct ggcgaaaatg agacgttgat
      241 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt
      301 tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
      361 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
      421 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
      481 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
      541 aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt
      601 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
      661 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
      721 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
      781 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatat
//
