LOCUS       sequence-43946-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS0a-RFP
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      161..232
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      248..275
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      329..400
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      416..443
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
       61 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac
      121 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
      181 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      241 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaag cggccgggat
      301 cctaactcga gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg
      361 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
      421 accttcgggt gggcctttct gcgtttatac ctagggtacg ggttttgctg cccgcaaacg
      481 ggctgttctg gtgttgctag tttgttatca gaatcgcaga tccggcttca gccggtttgc
      541 cggctgaaag cgctatttct tccagaattg ccatgatttt ttccccacgg gaggcgtcac
      601 tggctcccgt gttgtcggca gctttgattc gataagcagc atcgcctgtt tcaggctgtc
      661 tatgtgtgac tgttgagctg taacaagttg tctcaggtgt tcaatttcat gttctagttg
      721 ctttgtttta ctggtttcac ctgttctatt aggtgttaca tgctgttcat ctgttacatt
      781 gtcgatctgt tcatggtgaa cagctttgaa tgca
//
