LOCUS       sequence-43949-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbS0k-RFP
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      168..239
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      255..282
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      336..407
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      423..450
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 acggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac
       61 gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct
      121 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
      181 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
      241 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagaagcgg
      301 ccgggatcct aactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
      361 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      421 ctggctcacc ttcgggtggg cctttctgcg tttataccta gggtacgggt tttgctgccc
      481 gcaaacgggc tgttctggtg ttgctagttt gttatcagaa tcgcagatcc ggcttcagcc
      541 ggtttgccgg ctgaaagcgc tatttcttcc agaattgcca tgattttttc cccacgggag
      601 gcgtcactgg ctcccgtgtt gtcggcagct ttgattcgat aagcagcatc gcctgtttca
      661 ggctgtctat gtgtgactgt tgagctgtaa caagttgtct caggtgttca atttcatgtt
      721 ctagttgctt tgttttactg gtttcacctg ttctattagg tgttacatgc tgttcatctg
      781 ttacattgtc gatctgttca tggtgaacag ctttgaatgc accaaaaact cgtaaaagct
      841 ctgatgtatc tatctttttt aca
//
