LOCUS       sequence-43951-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbB0a-RFP
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             146..157
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(166..200)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     promoter        241..345
                     /gene="bla"
                     /label=AmpR promoter
     CDS             530..908
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(564..583)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcagtttagc ggtctgggta ccttcgtacg gacgaccttc accttcacct tcgatttcga
       61 actcgtgacc gttaacggaa ccttccatac gaactttgaa acgcatgaaa tctttgataa
      121 cgtcttcgga ggaagccatc tagtatttct cctctttcac tagtagctag cacagtacct
      181 aggactgagc tagctgtcaa agatctcatg aattcaggtg gcacttttcg gggaaatgtg
      241 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      301 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      361 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      421 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      481 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
      541 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
      601 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
      661 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
      721 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
      781 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
      841 gagctgaatg aagccatacc gaacgacgag cgtgacacca cgatgcctgt agcaatggca
      901 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttccc
//
