LOCUS       sequence-43953-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE0a-RFP
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      164..235
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      251..278
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      332..403
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      419..446
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(684..703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
       61 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
      121 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc
      181 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      241 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag aagcggccgg
      301 gatcctaact cgagtaagga tctccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      361 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      421 ctcaccttcg ggtgggcctt tctgcgttta tacctagggc gttcggctgc ggcgagcggt
      481 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      541 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      601 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      661 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      721 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcg
//
