LOCUS       sequence-43954-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbE0a-RFP
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             161..172
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(181..215)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     promoter        256..360
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(579..598)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cacctttggt aactttcagt ttagcggtct gggtaccttc gtacggacga ccttcacctt
       61 caccttcgat ttcgaactcg tgaccgttaa cggaaccttc catacgaact ttgaaacgca
      121 tgaactcttt gataacgtct tcggaggaag ccatctagta tttctcctct ttcactagta
      181 gctagcacag tacctaggac tgagctagct gtcaaagatc tcatgaattc aggtggcact
      241 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      301 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      361 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      421 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      481 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      541 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      601 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      661 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      721 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
      781 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aact
//
