LOCUS       sequence-439544-       10571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector that expresses Flag-tagged sgRNA resistant CIP2A 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Adam S, Rossi SE, Moatti N, De Marco Zompit M, Xue Y, Ng TF, 
            Alvarez-Quilon A, Desjardins J, Bhaskaran V, Martino G, Setiaputra 
            D, Noordermeer SM, Ohsumi TK, Hustedt N, Szilard RK, Chaudhary N, 
            Munro M, Veloso A, Melo H, Yin SY, Papp R, Young JTF, Zinda M, 
            Stucki M, Durocher D
  TITLE     The CIP2A-TOPBP1 axis safeguards chromosome stability and is a 
            synthetic lethal target for BRCA-mutated cancer.
  JOURNAL   Nat Cancer. 2021 Dec;2(12):1357-1371. doi: 
            10.1038/s43018-021-00266-w. Epub 2021 Nov 11.
  PUBMED    35121901
REFERENCE   2  (bases 1 to 10571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s43018-021-00266-w"; journalName: "Nat Cancer"; date: 
            "2021-12"; volume: "2"; issue: "12"; pages: "1357-1371"
COMMENT     Sequence Label: pHIV-NAT-FLAG-CIP2A FL (sg2R)
FEATURES             Location/Qualifiers
     source          1..10571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(490..1281,1282..1350))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1113..1132
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1351..1455)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1530..1549)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1721..2100
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2102..2300
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2255..2275
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2318..2498
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2545..2670
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3167..3400
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3585..3629
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3927..4044
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4107..5285
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4338..5276
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5233..5253
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5300..5323
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(8112..8686,8687..8689,8690..8698)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8279..8296)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8506..8525
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9278..9331
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(9534..9550)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9535..9551)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10060..10240
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(join(10302..10571,1..319))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10382..10401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
       61 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      121 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
      181 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      241 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      301 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      361 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      421 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
      481 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
      541 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
      601 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
      661 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
      721 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
      781 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
      841 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
      901 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
      961 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1021 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1081 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     1141 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     1201 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     1261 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1321 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1381 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1441 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     1501 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1561 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     1621 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     1681 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     1741 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     1801 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     1861 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     1921 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     1981 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2041 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2101 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2161 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2221 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2281 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
     2341 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     2401 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     2461 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     2521 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     2581 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     2641 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     2701 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     2761 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     2821 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     2881 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     2941 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     3001 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     3061 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     3121 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     3181 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     3241 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     3301 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     3361 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     3421 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     3481 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     3541 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     3601 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     3661 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     3721 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     3781 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     3841 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     3901 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     3961 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     4021 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     4081 ttagtaccgg gcccgctcta gcgtgaggct ccggtgcccg tcagtgggca gagcgcacat
     4141 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     4201 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     4261 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     4321 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     4381 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     4441 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     4501 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     4561 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     4621 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     4681 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     4741 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     4801 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     4861 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     4921 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     4981 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     5041 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     5101 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     5161 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     5221 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     5281 cgtgagcggc cgcaccatgg actacaaaga cgatgacgat aaagccgcca gtttaaacgg
     5341 cgcgccaatg gactccactg cctgcttgaa gtccttgctc ctgactgtca gtcagtacaa
     5401 agccgtgaag tcagaggcga acgccactca gcttttgcgg cacttggagg taatttctgg
     5461 acagaaactc acacgactat ttacatcaaa tcagatatta acaagtgaat gcttgagttg
     5521 ccttgtagag ctacttgaag accccaacat aagtgcttca ctgatcttaa gtattatcgg
     5581 tttgctgtct caactagcag tagacattga aaccagagat tgtcttcaga atacatataa
     5641 tctgaatagt gtgctggcgg gagtggtttg tcggagcagc cacactgatt cggtgttttt
     5701 gcagtgcatt caacttctac agaagttaac atataatgtc aaaattttct attctggtgc
     5761 caatatagat gaattaatta cgttcctgat agatcacatt caatcttctg aagatgagtt
     5821 aaaaatgcct tgtctaggat tattggcaaa tctttgtcgg cacaatcttt ctgttcaaac
     5881 gcacataaag acattgagta atgtgaaatc tttttatcga actcttatca ccttgttggc
     5941 ccatagtagt ttaactgtgg ttgtgtttgc actttcaata ttatccagtt tgacattaaa
     6001 tgaagaggtg ggggaaaagc tattccatgc tcgaaacatt catcagactt ttcaactaat
     6061 atttaatatt ctcataaacg gtgatggcac tctaactaga aagtattcag ttgacctact
     6121 gatggatctc cttaagaatc ctaaaattgc tgattatctc accagatatg agcacttttc
     6181 ttcatgtctt caccaagtat taggtcttct taatggaaag gatcctgatt cctcttcaaa
     6241 ggttttagaa ttacttcttg ccttctgttc agtgactcag ctgcgccata tgctcactca
     6301 gatgatgttt gaacagtctc cacctggcag cgccactctg ggaagccata ctaaatgttt
     6361 agaacctact gtggctctac tgcgctggtt aagccaacct ttggacggat cagaaaactg
     6421 ttctgtttta gcattggagt tgttcaagga aatatttgag gatgtcatag atgctgctaa
     6481 ctgttcctcg gctgatcgtt ttgtgaccct tctgctgcct acaatccttg atcaacttca
     6541 gttcacagaa caaaatctag atgaggcttt aacaagaaaa aaatgtgaaa ggattgccaa
     6601 ggccattgaa gttttgttaa ctctctgtgg agatgataca ctaaaaatgc atattgcaaa
     6661 aatcttgaca actgtcaagt gtaccactct tatagaacaa caatttacat atggcaagat
     6721 tgacctggga tttggaacaa aggttgcaga ttctgaatta tgcaaacttg ctgctgatgt
     6781 aattttgaaa actcttgatt tgattaacaa acttaaacca ttggttcctg gtatggaagt
     6841 aagcttctac aaaatacttc aggacccacg tttgattact cctttggctt ttgctttaac
     6901 gtcagataat agagaacaag tacagtctgg actgagaata ttattggagg ctgctccact
     6961 gccagatttt cctgctttag tacttggaga aagtatagca gcaaacaatg cctatagaca
     7021 acaggaaaca gaacatatac ccagaaaaat gccctggcaa tcatcaaatc acagttttcc
     7081 aacatcaata aagtgtttaa ctcctcattt gaaagatggt gttcctggat tgaatattga
     7141 agaattaata gagaaacttc agtctggaat ggtggtaaag gatcagattt gtgatgtgag
     7201 aatatctgac ataatggatg tatatgaaat gaaactatcc acattagctt ccaaagaaag
     7261 caggctacaa gatcttttgg aaacaaaagc tctagcactt gcacaggctg atagactgat
     7321 tgctcagcat cgctgtcaaa gaactcaagc tgaaacagag gcacggacac ttgctagtat
     7381 gttgagagaa gttgagagaa aaaatgaaga gcttagtgtg ttgctgaagg cgcagcaagt
     7441 tgaatcagaa agagcgcaga gtgatattga gcatctcttt caacataata ggaagttaga
     7501 gtctgtggct gaagaacatg aaatactgac aaaatcctac atggaacttc ttcagagaaa
     7561 tgaaagtact gaaaagaaga ataaagattt acagatcaca tgtgattctc tgaataaaca
     7621 aattgagaca gtgaaaaagt tgaatgagtc actcaaggaa caaaatgaaa aaagtattgc
     7681 ccaattaata gagaaagaag aacagagaaa agaagtacag aatcagctag tagacagaga
     7741 acataagcta gcaaatttgc atcaaaaaac aaaagtacaa gaagaaaaga ttaaaacctt
     7801 acaaaaggaa agggaagata aggaagaaac cattgatatc cttagaaaag aattaagcag
     7861 aacagaacag ataagaaaag agttgagcat taaggcttcc tccctagagg ttcaaaaggc
     7921 acaattagaa ggtcgtttgg aagagaaaga gtccttggtg aaacttcagc aagaggaatt
     7981 gaacaaacac tcccacatga tagcaatgat ccacagttta agtggtggaa aaataaatcc
     8041 agaaactgtg aatctcagta tataagaatt catcgacgtt aactattcta gagtacccgg
     8101 gctaggatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     8161 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     8221 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     8281 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     8341 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     8401 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     8461 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     8521 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     8581 gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     8641 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
     8701 gggcaccacc ctggacgaca cggcttaccg ataccgcacc agtgtccctg gggacgccga
     8761 ggccatcgag gcactggatg ggtccttcac caccgacacc gtcttccgcg tcaccgccac
     8821 cggggacggc ttcaccctgc gggaggtgcc ggtggacccg cccctgacca aggtgttccc
     8881 cgacgacgaa tcggacgacg aatcggacga cggggaggac ggcgacccgg actcccggac
     8941 gttcgtcgcg tacggggacg acggcgacct ggcgggcttc gtggtcgtct cgtactccgg
     9001 ctggaaccgc cggctgaccg tcgaggacat tgaggtagca cccgagcaca ggggacacgg
     9061 ggtgggacgg gcattgatgg gactggctac cgagttcgcc agagaacggg gagctggtca
     9121 tctttggctc gaagtcacca acgtcaacgc accggcgatc cacgcttacc ggcggatggg
     9181 gttcaccctc tgcggcctgg acaccgccct gtacgacggc accgcctcgg acggcgagca
     9241 ggcgctctac atgtccatgc cctgccccgg cagtggcgag ggcagaggaa gtctgctaac
     9301 atgcggtgac gtcgaggaga atcctggccc aatgaagcgc ttcctcttcc tcctactcac
     9361 catcagcctc ctggttatgg tacagataca aactggactc tcaggacaaa acgacaccag
     9421 ccaaaccagc agcccctcag catccagcaa cataagcgga ggcattttcc ttttcttcgt
     9481 ggctaatgcc ataatccacc tcttctgctt cagttgaggc gcgccaaatt tatcgatacc
     9541 gtcgacctcg atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     9601 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     9661 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     9721 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     9781 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     9841 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     9901 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     9961 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
    10021 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
    10081 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
    10141 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
    10201 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag
    10261 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10321 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10381 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10441 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10501 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10561 gaaccccccg t
//
