LOCUS       sequence-43955-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA0c-RFP
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             150..161
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(170..204)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     promoter        243..345
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(412..431)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 actttcagtt tagcggtctg ggtaccttcg tacggacgac cttcaccttc accttcgatt
       61 tcgaactcgt gaccgttaac ggaaccttcc atacgaactt tgaaacgcat gaactctttg
      121 ataacgtctt cggaggaagc catctagtat ttctcctctt tcactagtag ctagcacagt
      181 acctaggact gagctagctg tcaaagatct catgaattcg atatctggcg aaaatgagac
      241 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
      301 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      361 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      421 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      481 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      541 ctgatgaatg ctcatccgga atttcgtatg gcaatgaaag acggtgagct ggtgatatgg
      601 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      661 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
      721 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
      781 tc
//
