LOCUS       sequence-439556-       10376 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector that expresses Flag-tagged CIP2A NES mutant in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Adam S, Rossi SE, Moatti N, De Marco Zompit M, Xue Y, Ng TF, 
            Alvarez-Quilon A, Desjardins J, Bhaskaran V, Martino G, Setiaputra 
            D, Noordermeer SM, Ohsumi TK, Hustedt N, Szilard RK, Chaudhary N, 
            Munro M, Veloso A, Melo H, Yin SY, Papp R, Young JTF, Zinda M, 
            Stucki M, Durocher D
  TITLE     The CIP2A-TOPBP1 axis safeguards chromosome stability and is a 
            synthetic lethal target for BRCA-mutated cancer.
  JOURNAL   Nat Cancer. 2021 Dec;2(12):1357-1371. doi: 
            10.1038/s43018-021-00266-w. Epub 2021 Nov 11.
  PUBMED    35121901
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s43018-021-00266-w"; journalName: "Nat Cancer"; date: 
            "2021-12"; volume: "2"; issue: "12"; pages: "1357-1371"
COMMENT     Sequence Label: pHIV-NAT-FLAG-CIP2A ∆NES (del561-625)
FEATURES             Location/Qualifiers
     source          1..10376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    626..743
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1041..1085)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1270..1503
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2000..2125
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2172..2352
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2370..2568)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2395..2415)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2570..2949
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3121..3140
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3215..3319
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3320..3388,3389..4180)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3538..3557)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4351..4939
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4840..4859
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             5001..5181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     5690..5706
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5691..5707
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5910..5963)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(6543..6551,6552..6554,6555..7129)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6716..6735)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6945..6962
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(9723..9746)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(join(9761..10376,1..563))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          join(9770..10376,1..332)
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(9793..9813)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 cccgcattta caagactatc ttgccagaaa aaaagcgtcg cagcaggtca tcaaaaattt
       61 taaatggcta gagacttatc gaaagcagcg agacaggcgc gaaggtgcca ccagattcgc
      121 acgcggcggc cccagcgccc aggccaggcc tcaactcaag cacgaggcga aggggctcct
      181 taagcgcaag gcctcgaact ctcccaccca cttccaaccc gaagctcggg atcaagaatc
      241 acgtactgca gccaggtgga agtaattcaa ggcacgcaag ggccataacc cgtaaagagg
      301 ccaggcccgc gggaaccaca cacggcactt acctgtgttc tggcggcaaa cccgttgcga
      361 aaaagaacgt tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa
      421 aggcggagcc agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca
      481 ccggttcaat tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct
      541 gcccactgac gggcaccgga gcctcacgct agagcgggcc cggtactaac caaactggat
      601 ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt
      661 ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc
      721 caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtctg cagaattggc
      781 gcacgcagtg ccgatccgtt cactaatcga atggatctgt ctctgtctct ctctccacct
      841 tcttcttcta ttccttcggg cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac
      901 gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt
      961 cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt
     1021 gttatgttaa accaattcca caaacttgcc catttatcta attccaataa ttcttgttca
     1081 ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg
     1141 ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta
     1201 ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc
     1261 caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg
     1321 agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc
     1381 agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc
     1441 gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct
     1501 cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct
     1561 actcctaatg gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc
     1621 ctcatatctc ctcctccagg tctgaagatc agcggccggc cgcttgctgt gcggtggtct
     1681 tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta
     1741 tcctttgatg cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg
     1801 atcctgtctg aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt
     1861 ctaacaggcc aggattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt
     1921 ttaatttata ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa
     1981 ttctcccccg cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag
     2041 tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag
     2101 caagccgagt cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt
     2161 tcgggcgcca ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     2221 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     2281 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     2341 ccagagagac ccagtacagg caaaacgcgc tgcttatata gacctcccac cgtacacgcc
     2401 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     2461 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     2521 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     2581 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     2641 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     2701 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     2761 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     2821 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     2881 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctagtcaata
     2941 atcaatgtca acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
     3001 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
     3061 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
     3121 cggcatcaga gcagattgta ctgagagtgc accatagggg atcgggagat ctcccgatcc
     3181 gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3241 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3301 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     3361 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     3421 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     3481 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     3541 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     3601 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     3661 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3721 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3781 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3841 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3901 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3961 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4021 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4081 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4141 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4201 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     4261 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4321 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     4381 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     4441 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4501 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4561 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4621 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4681 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4741 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4801 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4861 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4921 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4981 ctggcctttt gctcacatgc tgctagagat tttccacact gactaaaagg gtctgaggga
     5041 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     5101 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     5161 ctggtctaac cagagagacc cagtacagtc cggatgcagc tctcgggcca tgtgatgaaa
     5221 tgctaggcgg ctgtcaaacc tccactctaa tacttctctc tccgggtcat ccatcccatg
     5281 caggctcaca gggtgtaaca agcgggtgtt ctctccttca ttggcttctt ctaccttctc
     5341 ttgctcaact ggtactagct tgtagcacca tccaaaggtc agtggatatc tgatccctgg
     5401 ccctggtgtg tagttctgcc aatcagggaa gtagccttgt gtgtggtaga tccacagatc
     5461 aaggatatct tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa
     5521 aaagtggcta agatctacag ctgccttgta agtcattggt cttaaaggta cctgaggtgt
     5581 gactggaaaa cccacctcct cctcctcttg tgcttctagc caggcacaat cagcattggt
     5641 agctgctgta ttgctacttg tgattgctcc atgtttttct aggtctcgat cgaggtcgac
     5701 ggtatcgata aatttggcgc gcctcaactg aagcagaaga ggtggattat ggcattagcc
     5761 acgaagaaaa ggaaaatgcc tccgcttatg ttgctggatg ctgaggggct gctggtttgg
     5821 ctggtgtcgt tttgtcctga gagtccagtt tgtatctgta ccataaccag gaggctgatg
     5881 gtgagtagga ggaagaggaa gcgcttcatt gggccaggat tctcctcgac gtcaccgcat
     5941 gttagcagac ttcctctgcc ctcgccactg ccggggcagg gcatggacat gtagagcgcc
     6001 tgctcgccgt ccgaggcggt gccgtcgtac agggcggtgt ccaggccgca gagggtgaac
     6061 cccatccgcc ggtaagcgtg gatcgccggt gcgttgacgt tggtgacttc gagccaaaga
     6121 tgaccagctc cccgttctct ggcgaactcg gtagccagtc ccatcaatgc ccgtcccacc
     6181 ccgtgtcccc tgtgctcggg tgctacctca atgtcctcga cggtcagccg gcggttccag
     6241 ccggagtacg agacgaccac gaagcccgcc aggtcgccgt cgtccccgta cgcgacgaac
     6301 gtccgggagt ccgggtcgcc gtcctccccg tcgtccgatt cgtcgtccga ttcgtcgtcg
     6361 gggaacacct tggtcagggg cgggtccacc ggtacctccc gcagggcgaa gccgtccccg
     6421 gtggcggtga cgcggaagac ggtgtcggtg gtgaaggacc catccagtgc ctcgatggcc
     6481 tcggcgtccc cagggacact ggtgcggtat cggtaagccg tgtcgtccag ggtggtgccc
     6541 atggttgtgg ccatattatc atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg
     6601 ggggcctaga cgttttttta acctcgacta aacacatgta aagcatgtgt accgaggccc
     6661 cagatcagat cccatacaat ggggtacctt ctgggcatcc ttcagcccct tgttgaatac
     6721 gcttgaggag agccatttga ctctttccac aactatccaa ctcacaacgt ggcactgggg
     6781 ttgtgccgcc tttgcaggtg tatcttatac acgtggcttt tggccgcaga ggcacctgtc
     6841 gccaggtggg gggttccgct gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga
     6901 agcttccaga ggaactgctt ccttcacgac attcaacaga ccttgcattc ctttggcgag
     6961 aggggaaaga cccctaggaa tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac
     7021 attgccaaaa gacggcaata tggtggaaaa taacatatag acaaacgcac accggcctta
     7081 ttccaagcgg cttcggccag taacgttagg ggggggggag ggagaggggc ggatcctagc
     7141 ccgggtactc tagaatagtt aacgtcgatg aattcttata tactgagatt cacagtttct
     7201 ggatttattt ttccaccact taaactgtgg atcattgcta tcatgtggga gtgtttgttc
     7261 aattcctctt gctgaagttt caccaaggac tctttctctt ccaaacgacc ttctaattgt
     7321 gccttttgaa cctctaggga ggaagcctta atgctcaact cttttcttat ctgttctgtt
     7381 ctgcttaatt cttttctaag gatatcaatg gtttcttcct tatcttccct ttccttttgt
     7441 aaggttttaa tcttttcttc ttgtactttt gttttttgat gcaaatttgc tagcttatgt
     7501 tctctgtcta ctagctgatt ctgtacttct tttctctgtt cttctttctc tattaattgg
     7561 gcaatacttt tttcattttg ttccttgagt gactcattca actttttcac tgtctcaatt
     7621 tgtttattca gagaatcaca tgtgatctgt aaatctttat tcttcttttc agtactttca
     7681 tttctctgaa gaagttccat gtaggatttt gtcagtattt catgttcttc agccacagac
     7741 tctaacttcc tattatgttg aaagagatgc tcaatatcac tctgcgctct ttctgattca
     7801 acttgctgcg ccttcagcaa cacactaagc tcttcatttt ttctctcaac ttctctcaac
     7861 atactagcaa gtgtccgtgc ctctgtttca gcttgagttc tttgacagcg atgctgagca
     7921 atcagtctat cagcctgtgc aagtgctaga gcttttgttt ccaaaagatc ttgtagcctg
     7981 ctttctttgg aagctaatgt ggatagtttc atttcatatt cctgttgtct ataggcattg
     8041 tttgctgcta tactttctcc aagtactaaa gcaggaaaat ctggcagtgg agcagcctcc
     8101 aataatattc tcagtccaga ctgtacttgt tctctattat ctgacgttaa agcaaaagcc
     8161 aaaggagtaa tcaaacgtgg gtcctgaagt attttgtaga agcttacttc cataccagga
     8221 accaatggtt taagtttgtt aatcaaatca agagttttca aaattacatc agcagcaagt
     8281 ttgcataatt cagaatctgc aacctttgtt ccaaatccca ggtcaatctt gccatatgta
     8341 aattgttgtt ctataagagt ggtacacttg acagttgtca agatttttgc aatatgcatt
     8401 tttagtgtat catctccaca gagagttaac aaaacttcaa tggccttggc aatcctttca
     8461 catttttttc ttgttaaagc ctcatctaga ttttgttctg tgaactgaag ttgatcaagg
     8521 attgtaggca gcagaagggt cacaaaacga tcagccgagg aacagttagc agcatctatg
     8581 acatcctcaa atatttcctt gaacaactcc aatgctaaaa cagaacagtt ttctgatccg
     8641 tccaaaggtt ggcttaacca gcgcagtaga gccacagtag gttctaaaca tttagtatgg
     8701 cttcccagag tggcgctgcc aggtggagac tgttcaaaca tcatctgagt gagcatatgg
     8761 cgcagctgag tcactgaaca gaaggcaaga agtaattcta aaacctttga agaggaatca
     8821 ggatcctttc cattaagaag acctaatact tggtgaagac atgaagaaaa gtgctcatat
     8881 ctggtgagat aatcagcaat tttaggattc ttaaggagat ccatcagtag gtcaactgaa
     8941 tactttctag ttagagtgcc atcaccgttt atgagaatat taaatattag ttgaaaagtc
     9001 tgatgaatgt ttcgagcatg gaatagcttt tcccccacct cttcatttaa tgtcaaactg
     9061 gataatattg aaagtgcaaa cacaaccaca gttaaactac tatgggccaa caaggtgata
     9121 agagttcgat aaaaagattt cacattactc aatgtcttta tgtgcgtttg aacagaaaga
     9181 ttgtgccgac aaagatttgc caataatcct agacaaggca tttttaactc atcttcagaa
     9241 gattgaatgt gatctatcag gaacgtaatt aattcatcta tattggcacc agaatagaaa
     9301 attttgacat tatatgttaa cttctgtaga agttgaatgc actgcaaaaa caccgaatca
     9361 gtgtggctgc tccgacaaac cactcccgcc agcacactat tcagattata tgtattctga
     9421 agacaatctc tggtttcaat gtctactgct agttgagaca gcaaaccgat aatacttaag
     9481 atcagtgaag cacttatgtt ggggtcttca agtagctcta caaggcaact caagcattca
     9541 cttgttaata tctgatttga tgtaaatagt cgtgtgagtt tctgtccaga aattacctcc
     9601 aagtgccgca aaagctgagt ggcgttcgcc tctgacttca cggctttgta ctgactgaca
     9661 gtcaggagca aggacttcaa gcaggcagtg gagtccattg gcgcgccgtt taaactggcg
     9721 gctttatcgt catcgtcttt gtagtccatg gtgcggccgc tcacgacacc tgaaatggaa
     9781 gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa
     9841 gggcaaattc caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc
     9901 actcagtgtg gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac
     9961 tccaaaagct cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac
    10021 atgaagcgac ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc
    10081 gctctcccga gcgccgcgtc ctccattttg agctccctgc agcagggccg ggaagcggcc
    10141 atctttccgc tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga
    10201 tacacggcgg cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc
    10261 cgattctcgg tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac
    10321 gggccccgtc gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttgg
//
