LOCUS       sequence-439560-        4877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 
            5-HT7R coding frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM
  TITLE     Serotonin acts through multiple cellular targets during an olfactory
            critical period.
  JOURNAL   bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413.
  PUBMED    38645269
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413."
COMMENT     Sequence Label: pTwist Amp_d5-HT7R-7xsfGFP11-HA-HDR-donor
FEATURES             Location/Qualifiers
     source          1..4877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1226..1273
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1307..1354
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1370..1417
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1433..1480
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1496..1543
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1559..1606
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1622..1669
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             1673..1699
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(2716..2732)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2716..2732)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2729..2751)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2904..3492)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2984..3003)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(3609..3633)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(3733..4524,4525..4593))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSATITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4356..4375
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4594..4698)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4797..4819
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4811..4828
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4812..4828
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattatgcta gccactgtag tgggcaatgt cctggtctgc atagccgttt gcatggtgcg
       61 gaaattgagg agaccctgca attacctttt ggtgtccttg gctctttcgg atctctgtgt
      121 ggctcttctg gtgatgccca tggccttgct ttatgaagtg ctggagaagt ggaactttgg
      181 accgctgctc tgcgacatct gggtgtcctt cgatgtgctg tgctgcacgg cctcgatcct
      241 gaatctgtgt gccatatccg tggatcgata cctggccatc acgaagcccc tggagtacgg
      301 cgtgaagagg actcctcggc gaatgatgct gtgcgtgggc atcgtctggc tggccgccgc
      361 ctgcatctca ttgcctcctc tgctgatcct gggcaacgag cacgaggacg aggagggcca
      421 gcctatctgc acggtgtgcc agaacttcgc atatcagatc tacgccaccc tgggctcatt
      481 ttacatacca ctctcggtga tgctgtttgt gtactaccag atcttcaggg cggcgaggcg
      541 gattgtcctg gaagagaaac gcgcccagac gcatttgcaa caggcgctca atggcacagg
      601 atcgccatcg gccccgcagg ctccgccctt gggccacacg gagctggcca gctcgggaaa
      661 tggccagagg cacagcagcg tgggcaacac ttcgctcacc tactccacct gtgggggtct
      721 gagcagcgga ggaggcgcac tggctgggca cggcagtggg ggtggcgtta gtggttcgac
      781 cggactcctg ggctctccgc accacaagaa gctgcggttt caactggcca aagagaagaa
      841 ggcctccacc actctgggca tcatcatgtc ggcctttacc gtctgctggc tgccattctt
      901 tatcctggcc ctaattcgtc ctttcgagac aatgcacgta ccggcatcgc tctcgtctct
      961 attcctctgg ctgggctatg ccaactccct gctgaaccct attatctacg ccactctgaa
     1021 cagggatttc cgcaaaccct tccaggagat cctctacttc cgctgctcca gcctgaacac
     1081 catgatgcgg gagaactact accaggatca gtatggcgag cctccctcgc agcgggtgat
     1141 gctgggcgat gagaggcacg gggcgaggga gagctttctc ggcgatggag gaagcggagg
     1201 cgacggaggc agcggaggag gcagccggga ccacatggtg ctgcacgagt acgtgaacgc
     1261 cgccggcatc actagcggag gcgacggagg cagcggagga ggcagccggg accacatggt
     1321 gctgcacgag tacgtgaacg ccgccggcat cactagcggt agtggcggcc gtgaccacat
     1381 ggtccttcat gagtatgtaa atgctgctgg gattacaggt ggctctggag gtagagatca
     1441 tatggttctc cacgaatacg ttaacgccgc aggcatcact ggcggtagtg gaggacgcga
     1501 ccatatggta ctacatgaat atgtcaatgc agccggaata accggagggt ccggaggccg
     1561 ggatcacatg gtgctgcatg agtatgtgaa cgcggcgggt ataactggtg ggtcgggcgg
     1621 acgagaccat atggtgcttc acgaatacgt aaacgcagct ggcattacta gttacccata
     1681 cgatgttcca gattacgctt aactctagac catccacgag gacctccatt ccggagaaag
     1741 gagcctatcc aagcgaatct gagctgctcg aagggtcttc cctaggtttc cggaagaagc
     1801 gaactcctct gctcgacaca ctcacacact taggtataaa cactggggtg tacatcttaa
     1861 acaattagga aatgtaaagt tttaagtggg aaaatcaggc tctgggctgt ttaaagtacc
     1921 gcgttggtat agtttattta gcagaaataa tcatctaata ttatttgtaa ctgagagttt
     1981 gatcatgtcc gttaattgtc tataaattaa ttttctatta aatttaacga tatgtttata
     2041 agggttacat tgattgcaga ccccccattg actcaaaaga aaacgtacgg aaagcagagc
     2101 aaatattacc tttccccgca tattgccacc accccagtac acacacatgg atatttgtat
     2161 gtagtagtgc tagtagcgta gcgtatctct gtatatgtgc accccctgaa acttgcagca
     2221 atgtgcgaat tgtagacgac tcctctaaat gtaacttgtt gttgtaattt taaatgtaga
     2281 actaagcaat cgacgtggtg cgtgccacgc acacaatcac actcaccaac caatcataca
     2341 cacacacaca cacacggttg taactgtgac aggtttctac ggttcctttt atgtttcgaa
     2401 aacaatcagc accggatatg agacacaatc gttatcgaat ctattgatac ctcccagaac
     2461 aagaaaaaga tcccacagtc cccatcccaa aatttgccag cgtagttagt tgatttgcga
     2521 tactcaaact gagccaaaaa ttaaatttac aaactgcaga ttgcagccaa attaagccta
     2581 ctgattaacc tagatatatt tacgttttga gcaagaagct atccactgta aatacccagc
     2641 cccctgcggc cgcaattata ggctaggtgg aggctcagtg atgataagtc tgcgatggtg
     2701 gatgcatgtg tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcagagggca
     2761 caatcctatt ccgcgctatc cgacaatctc caagacatta ggtggagttc agttcggcgt
     2821 atggcatatg tcgctggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2881 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2941 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3001 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3061 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3121 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3181 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3241 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3301 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3361 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3421 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3481 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctct attcaacaaa
     3541 gccgccgtcc cgtcaagtca gcgtaaatgg gtagggggct tcaaatcgtc ctcgtgatac
     3601 caattcggag cctgcttttt tgtacaaact tgttgataat ggcaattcaa ggatcttcac
     3661 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3721 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3781 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3841 accatctggc cccagtgctg caatgatacc gcgagagcca cgctcaccgg ctccagattt
     3901 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3961 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4021 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4081 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4141 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4201 agtgttatca ctcatggtta tggtagcact gcataattct cttactgtca tgccatccgt
     4261 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4321 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4381 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4441 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4501 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4561 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4621 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4681 acaaataggg gttccgcgca catttccccg aaaagtgcca gatacctgaa acaaaaccca
     4741 tcgtacggcc aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca
     4801 gtcacgacgt tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaaggaagt
     4861 gccattccgc ctgacct
//
