LOCUS       sequence-43957-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA0k-RFP
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      156..227
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      243..270
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      324..395
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      411..438
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(466..483)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(680..699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc
       61 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga
      121 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      181 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      241 ctggctcacc ttcgggtggg cctttctgcg tttatatact agaagcggcc gggatcctaa
      301 ctcgagtaag gatctccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      361 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      421 cgggtgggcc tttctgcgtt tatacctagg gatatattcc gcttcctcgc tcactgactc
      481 gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg gcggagattt
      541 cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc aaagccgttt
      601 ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg
      661 cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc cctcgtgcgc
      721 tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcgtttgtct
      781 cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg actgtatg
//
