LOCUS       sequence-43959-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA0a-RFP
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      154..225
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      241..268
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      322..393
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      409..436
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(464..481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(678..697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat
       61 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata
      121 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
      181 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
      241 ggctcacctt cgggtgggcc tttctgcgtt tatatactag aagcggccgg gatcctaact
      301 cgagtaagga tctccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      361 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      421 ggtgggcctt tctgcgttta tacctaggga tatattccgc ttcctcgctc actgactcgc
      481 tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc
      541 tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt
      601 ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg
      661 aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc
      721 tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca
      781 ttccacgcct gacactcagt tccgggtagg cagttcgctc cagctggact gtatgcacga
//
