LOCUS       sequence-43960-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Lee TS, Krupa RA, Zhang F, Hajimorad M, Holtz WJ, Prasad N, Lee SK, 
            Keasling JD
  TITLE     BglBrick vectors and datasheets: A synthetic biology platform for 
            gene expression.
  JOURNAL   J Biol Eng. 2011 Sep 20;5:12.
  PUBMED    21933410
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2011-09-20"; volume: "5"; pages: "12"
COMMENT     Sequence Label: pBbA0a-RFP
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             157..168
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(177..211)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     promoter        252..356
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(575..594)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttggtaact ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc
       61 ttcgatttcg aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa
      121 ctctttgata acgtcttcgg aggaagccat ctagtatttc tcctctttca ctagtagcta
      181 gcacagtacc taggactgag ctagctgtca aagatctcat gaattcaggt ggcacttttc
      241 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      301 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      361 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      421 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      481 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      541 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      601 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      661 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      721 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      781 gaccgaagga gctaaccgct tttttgcaca acatg
//
