LOCUS       sequence-43961-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal 
            PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, 
            Schlaeger TM, Rossi DJ
  TITLE     Highly efficient reprogramming to pluripotency and directed 
            differentiation of human cells with synthetic modified mRNA.
  JOURNAL   Cell Stem Cell. 2010 Nov 5;7(5):618-30. doi: 
            10.1016/j.stem.2010.08.012. Epub 2010 Sep 30.
  PUBMED    20888316
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2010.08.012"; journalName: "Cell Stem Cell"; date: 
            "2010-11-5- 5"; volume: "7"; issue: "5"; pages: "618-30"
COMMENT     Sequence Label: pcDNA3.3_LIN28A
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ccggtaggga tcgaaccctt ttggaccctc gtacagaagc taatacgact cactataggg
       61 aaataagaga gaaaagaaga gtaagaagaa atataagagc caccatgggc tccgtgtcca
      121 accagcagtt tgcaggtggc tgcgccaagg cggcagaaga ggcgcccgag gaggcgccgg
      181 aggacgcggc ccgggcggcg gacgagcctc agctgctgca cggtgcgggc atctgtaagt
      241 ggttcaacgt gcgcatgggg ttcggcttcc tgtccatgac cgcccgcgcc ggggtcgcgc
      301 tcgacccccc agtggatgtc tttgtgcacc agagtaagct gcacatggaa gggttccgga
      361 gcttgaagga gggtgaggca gtggagttca cctttaagaa gtcagccaag ggtctggaat
      421 ccatccgtgt caccggacct ggtggagtat tctgtattgg gagtgagagg cggccaaaag
      481 gaaagagcat gcagaagcgc agatcaaaag gagacaggtg ctacaactgt ggaggtctag
      541 atcatcatgc caaggaatgc aagctgccac cccagcccaa gaagtgccac ttctgccaga
      601 gcatcagcca tatggtagcc tcatgtccgc tgaaggccca gcagggccct agtgcacagg
      661 gaaagccaac ctactttcga gaggaagaag aagaaatcca cagccctacc ctgctcccgg
      721 aggcacagaa ttgagctgcc ttctgcgggg cttgccttct ggccatgccc ttcttctctc
      781 ccttgcacct gtacctcttg gtctttgaat aaagcctgag taggaagtga gggtctagaa
      841 ctagtgtcga cgcaagggtt cgatcctcta gagtc
//
