LOCUS       sequence-439689-        5277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 
            5-HT2AR isoforms B, F and H coding frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5277)
  AUTHORS   Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM
  TITLE     Serotonin acts through multiple cellular targets during an olfactory
            critical period.
  JOURNAL   bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413.
  PUBMED    38645269
REFERENCE   2  (bases 1 to 5277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413."
COMMENT     Sequence Label: pTwist
            Amp_d5-HT2AR(isoformBFH)-7xsfGFP11-HA-H...
FEATURES             Location/Qualifiers
     source          1..5277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1447..1464)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1447..1463)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1456..1478)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1577..1681
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1682..1750,1751..2542)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSATITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1900..1919)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    2642..2666
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      2783..3371
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3272..3291
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3524..3546
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3543..3559
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3543..3559
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(4842..4868)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(4872..4919)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(4935..4982)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(4998..5045)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(5061..5108)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(5124..5171)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(5187..5234)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(join(5268..5277,1..38))
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 gcggcgttca cgtactcgtg cagcaccatg tggtcccggc tgcctcctcc gctgcctccg
       61 tcgcctccgc ttcctccatc gccgcactcg tcgtctgtga ctgtgcggca gctggcgcgg
      121 aactcgctca tcggcgtcgc attagccgta gatggcgtca taagtggcgc cccctggaag
      181 gatatggcca gcggcagggc cgaaggggcg cacaggctga gagcgccgcg tgcttctgtc
      241 acagaccgat agcgcagcgg gcggccggaa ctgcaatggg atggcaataa ctacgttatt
      301 ggctgtgcca ttttaagcca tttcgagtcg attgggtttt aaggcagaag tcagtgacag
      361 aaaagtgctc tcgtcttggt aaatgtccta tacttttcaa tacgttttta attcgaacac
      421 aaggcatttt aaataaattt gacattcgac acagtttgtt tttaatgctt acgaaattta
      481 tgtcttacaa tgtgtggcga ttctagcaag tctttaaaat agataaaagt cgtattacaa
      541 agattcccat tcattctgca tattaaatac aaacaatcga acttattctt cctttaatat
      601 attttgagag cgtttgattt cctatcgctg cttattcatt cgctgccaca accaccccga
      661 gttcgtctct tgtctggatt cctctggtaa atcaaaaccc caaaaatcaa tcatacgtca
      721 cgttgcacat taattcccag cagaatgcac tcttagagca ataaattgca tccctgtgtc
      781 actgtaacct gcagcgacga attttagttc gaggtgcctt cgtcggttgg gagaggcaat
      841 ttcttactgt ggcactggat ccgcgtctgg gctgcagtga cttctcactg ctctactact
      901 caccgtttgc agttgcactt gagcaggcgg ataaaggccg cccggaacgt gcgattgaat
      961 atggtgtaga tgatgggatt gatcgtcgag gagacatagc caagccaaag gcaggtattc
     1021 accacgtgct ccggcacttg gcactcgggg catgcggcaa atataatgtt caagatgaag
     1081 aaaggcgacc agcacagaac gaaggtgaaa aacaccaatc cgagcacctt ggtggccttc
     1141 tgctccgtag caacggcatt ggccgacaag ctgtttcgtt tactgcgatt gtttagaaaa
     1201 ctgtaaggca aaagtggata aaagatatta gtagtaccaa aaagttaagc catgatgatt
     1261 attgtttcat tattttctca tctagtgaga gcgcgagaaa gtcacatcta tcagaaaccc
     1321 atagttatat aaacaagaga gaacgttata gtcaagttcc tcgactgtca gatacccgtt
     1381 actcagctag cataattagg tcaggcggaa tggcacttcc ttccagatgc gtgcggatta
     1441 tgcatgactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg cttgtggtgg
     1501 atcacagtta ttggagactt ccttggccgt acgatgggtt ttgtttcagg tatctggcac
     1561 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1621 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     1681 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     1741 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1801 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1861 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1921 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1981 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     2041 atgcagtgct accataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     2101 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     2161 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     2221 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     2281 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2341 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggctc
     2401 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2461 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     2521 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     2581 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atccttgaat tgccattatc
     2641 aacaagtttg tacaaaaaag caggctccga attggtatca cgaggacgat ttgaagcccc
     2701 ctacccattt acgctgactt gacgggacgg cggctttgtt gaatagagcg tcagaccccg
     2761 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2821 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2881 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2941 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3001 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3061 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3121 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3181 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3241 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3301 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3361 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3421 ttgctcacat gttctttcca gcgacatatg ccatacgccg aactgaactc cacctaatgt
     3481 cttggagatt gtcggatagc gcggaatagg attgtgccct ctgagcggat aacaatttca
     3541 cacaggaaac agctatgacc atgacacatg catccaccat cgcagactta tcatcactga
     3601 gcctccacct agcctataat tgcggccgct cggaaacgct tccttctgcc tgtaaaatat
     3661 atttcaaagt atttagtata cccttcttcc ttacgagtta ttacgagtaa tacgtgtatt
     3721 gatttattta cgagtattaa tattatgaat attccctatt caagtgtcac tttattttat
     3781 aaaagccggc gctctgttca aaggatattt tagttgaata aaaaataatt tgtcaaccca
     3841 atggtgatat cggaacttat gtatgtggga ggtgcggaag gattacatcc ttaagcggat
     3901 tttttgttgg ttcaaaactt ttttcattag gctagccgta aaatatatgt ctatgtagaa
     3961 ttaaaaaaga aagccgaatc ttacaaactg gttatcaata aaagggaaac taaattatat
     4021 attatctatg aaaacagcga agctaagatt tctttccatt aattaggtag taattctcag
     4081 actatgacag ctttgtacta taaagataca tttctttaaa gttgattttt gatcgttcct
     4141 ataacagttg catatatgta tgttgtaggc atccaattct gcctgttccg atttatacga
     4201 gtatgctacc aatttaaact actacgaact aaaaggtata aggcggacta tatcgacctg
     4261 gctgttttta ctgataaagt gtatacaaat atattttttc cagatccagc tgctagcaaa
     4321 aggcgcttat tggaatcatt tattttgctt atttgcaaaa gaaaagatta caatttgttt
     4381 ataaatttgc atataacgaa cgactcttac tcccactata aactaaaggg taaaaaaact
     4441 aatactttta ccttcttttt cttattttat tttgcactaa tcgtacttat tgttaatgat
     4501 tttttgtttg acttagaaat aacgcaatga ccaagtctag aatgttgatg agaaagtgaa
     4561 aaacagtccg cgttgtaccg caaactctct cactatattt ttgaaccttc ctttgtttct
     4621 aatgatatgc gatgagactt aagttaggta ttttctctat agcatctaca aaattcatca
     4681 aattgattgt gattgaaaag gttagcattg atctccctga agttatctag gaaaagccgc
     4741 taattacagt gaactccgtt ttgtttctgc cgatacgtcg atataaattt gatgcatccc
     4801 agacgctgga tggtgctcct cgtggttcag gtctagagtt aagcgtaatc tggaacatcg
     4861 tatgggtaac tagtaatgcc agctgcgttt acgtattcgt gaagcaccat atggtctcgt
     4921 ccgcccgacc caccagttat acccgccgcg ttcacatact catgcagcac catgtgatcc
     4981 cggcctccgg accctccggt tattccggct gcattgacat attcatgtag taccatatgg
     5041 tcgcgtcctc cactaccgcc agtgatgcct gcggcgttaa cgtattcgtg gagaaccata
     5101 tgatctctac ctccagagcc acctgtaatc ccagcagcat ttacatactc atgaaggacc
     5161 atgtggtcac ggccgccact accgctagtg atgccggcgg cgttcacgta ctcgtgcagc
     5221 accatgtggt cccggctgcc tcctccgctg cctccgtcgc ctccgctagt gatgccg
//
