LOCUS       sequence-439690-        4780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for inserting 2xFLAG at the beginning of Drosophila 
            SERT coding frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM
  TITLE     Serotonin acts through multiple cellular targets during an olfactory
            critical period.
  JOURNAL   bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413.
  PUBMED    38645269
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413."
COMMENT     Sequence Label: pTwist Amp_2xFlag-dSERT-HDR-donor-HDR
FEATURES             Location/Qualifiers
     source          1..4780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1211..1228)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1211..1227)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1220..1242)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1341..1445
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1446..1514,1515..2306)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSATITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1664..1683)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    2406..2430
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      2547..3135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3036..3055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3288..3310
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3307..3323
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3307..3323
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(4496..4519)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(4532..4555)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgtttagcgc tgtggtcgtg gaaattgcat tttcccacgg ctcctcacat gctggcctcg
       61 ctgaatcgac tggcaattaa ctgcaaccgg agttaagcaa tcgcattaaa tggggctgca
      121 ctaacgatgg ccatttgttt ctccccaagg gaggcattca aagtagcaaa ttttgcactg
      181 atttccgacc gatttgggat gcccaatata gtggttaaaa aacatttaaa tgctgcggtc
      241 acgggcattg cagcggtttt aaacgctgcc atggcctcca aagaaatatt caatgccaat
      301 gtcgtaaaat attgcgattt cggttaacta ctttcggggc ttatacttaa tttacacttt
      361 aaattacgca ctgtacacca cggcgtatgc ttaacgagat tcatgcgttt tcaatgcagc
      421 cttgatcttc tttcaaaccc ctttcccaac tctcaaacgt tttaattgcc atttaattgt
      481 ggcccacaca cgaagccaaa cagcagataa gatgaagaaa gtggccaaat gtgccgacgg
      541 aatttcctaa tgagagcgtt ctgataagac aagtgctaaa aatgcgaccc gaaaagcgaa
      601 actcaaaaca attaaaagcc acttagagat atggagttat tctccaggtg cttcacatca
      661 gatatttgaa caaatggaag tctgattgat attaaaaatg tatcaaattt tttctaaatg
      721 aatcgctttt gtaactaaac tactatttca aatagaacgc tttgcttgaa cttaagaaat
      781 atagtttaag caacataaat cattgacaca agtaatgttt ttaagaaact attacaatgg
      841 aatatggggg tttagactta cttgttggat ctaacttaca aagatatttt ccttgattga
      901 gatgatttta attatttcac tttgaattaa gcactttgaa caacttccaa ggcactgcac
      961 tggtggtttc tggtgcttct ggcttccatg cgtccgactt gagagacgcg cgccgctcct
     1021 ttgggccaac tggtgagtcc tgctgccgga tggctccttt tatacgctgg atgcgcaggc
     1081 gggcgccgat ttgtcgtcgg caggccgagt gtccttcggc tgtggccact gcgtcctctg
     1141 gggttggggg ctagcataat taggtcaggc ggaatggcac ttccttccag atgcgtgcgg
     1201 attatgcatg actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgcttgtg
     1261 gtggatcaca gttattggag acttccttgg ccgtacgatg ggttttgttt caggtatctg
     1321 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1381 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1441 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1501 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1561 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1621 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1681 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1741 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1801 aattatgcag tgctaccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1861 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1921 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1981 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2041 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2101 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2161 gctctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2221 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2281 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2341 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt gaattgccat
     2401 tatcaacaag tttgtacaaa aaagcaggct ccgaattggt atcacgagga cgatttgaag
     2461 ccccctaccc atttacgctg acttgacggg acggcggctt tgttgaatag agcgtcagac
     2521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3121 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3181 ccttttgctc acatgttctt tccagcgaca tatgccatac gccgaactga actccaccta
     3241 atgtcttgga gattgtcgga tagcgcggaa taggattgtg ccctctgagc ggataacaat
     3301 ttcacacagg aaacagctat gaccatgaca catgcatcca ccatcgcaga cttatcatca
     3361 ctgagcctcc acctagccta taattgcggc cgcttgataa tgtcgccggc acacacaatg
     3421 aatggttcct tttttgaaag gccccaaact ttgctccttt gaggtcctgc ggcccacaag
     3481 tgcgggtgtc ctcggagcct ggctgagaca atattgctcg gccgagcgtg aaatatcaga
     3541 catcaacaac acacgagctc atgttccgta cgcttttcgc agggccacgc tgcgtatact
     3601 taatttctca ttacaaaatg ctggagccgc aggcgaaatc agtgcgtaaa tattgaatgg
     3661 cctatggatt ccaatatgca atcatgtttt ttacgttctg cgactaaaac tcaatatttg
     3721 aaattcattt aaattgccat ttaatccgga atatgttcgc aagccccagg cgcatccatg
     3781 tttatttgcc catcattttt caaggccatg gttacatggt tacatggtta cagtacattt
     3841 gttagagaaa caacttccag tttttacact caccttttag ggccggacag atgcgcttcc
     3901 agatgctgag gcaaccacat cggtggaact ggcccagcgc cagctccatg tagaacaggg
     3961 gcagtccacc gaagatgagg aagaggcagt agggcaccag gaaggcgccg cctccgttct
     4021 gatagcagat gtacgggaag cgccacacat tgcccagatc cactgcgaac ccaatcacgg
     4081 ccaggaggaa ctccgccttc tgtccccagg tctcgcgagt cctctccgtc acggagacca
     4141 ccagaatgcg ctgaatggga cacaaacatg atggtacaag gatggaggtt ttagcttatg
     4201 gaataaaata tatctcggat catccgacaa tccatccgtc cattaattcc atatgaactg
     4261 gtgcttatat gctaatggtt aagtcaaggt aactcacctc gttgttggcc agcttggggg
     4321 ccagtggatc ggtgaccttg gcgggcgtcg tgtggtcgcc atcgtcctcg ttggagtcgt
     4381 cctcgttgtt tggcgactct ttgtcgtcgc tccagggcgc gggattcgag cggcccgtcg
     4441 tgacagctgc tcctgcgaaa tcggagctcc cgctgcggtc catgcccgat ccacctttgt
     4501 catcgtcgtc cttatagtcg ctgccggatc ccttatcgtc atcatctttg taatccatct
     4561 tgtggtgcgt ggggcgtgac aggggcgggg gcgcggtgcc tggaggcgtg gcagtgtacg
     4621 ccgatcgcgg cacgagctta gccagccgaa ctctggatct gcactcagtc cggggccaga
     4681 aaaagcgaaa tctgcgctct acttgcgact tttccctgct cttcactgct cctcgcccac
     4741 ctgctgtccc tatcttttcc gacttcccgc ttttccggcc
//
