LOCUS       sequence-439808-       11119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression vector of 53BP1 28A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11119)
  AUTHORS   Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, 
            Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D
  TITLE     A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and
            BRCA1-CtIP controls DNA repair pathway choice.
  JOURNAL   Mol Cell. 2013 Mar 7;49(5):872-83. doi: 
            10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.
  PUBMED    23333306
REFERENCE   2  (bases 1 to 11119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.01.001"; journalName: "Mol Cell"; date: 
            "2013-03-7- 7"; volume: "49"; issue: "5"; pages: "872-83"
COMMENT     Sequence Label: pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A
FEATURES             Location/Qualifiers
     source          1..11119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..2313)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     protein_bind    complement(2321..2368)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     2500..2519
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2652..2876
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2865..2882
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    2924..2948
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8871..8895)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(8896..8919)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9060..9084)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    9094..9112
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9115..9133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(9135..9338)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9164..9184)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9339..9718
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9890..9909
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9984..10088
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10089..10157,10158..10949)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10307..10326)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagtacgtg ctattccttt gccctcggac gagtgctggg
     1321 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     1381 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     1441 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     1501 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     1561 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     1621 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     1681 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     1741 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     1801 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     1861 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     1921 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     1981 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     2041 ggagatgcaa taggtcaggc tctcgctgaa ctccccaatg tcaagcactt ccggaatcgg
     2101 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     2161 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     2221 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     2281 ctcgacagac gtcgcggtga gttcaggctt tttggccaag gaagttccta tactttctag
     2341 agaataggaa cttcggaata ggaacttcta ggtacgtgaa ccatcaccct aatcaagttt
     2401 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     2461 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     2521 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     2581 gcttaatgcg ccgctacagg gcgcgtgggg atacccccta gagccccagc tggttctttc
     2641 cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt cttcccaatc
     2701 ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca
     2761 atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac cttccagggt
     2821 caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag gcacagtcga
     2881 ggctgatcag cgggtttaaa cgggccctct agactcgagt cagaccactt tgtacaagaa
     2941 agctgggtct tagtgagaaa cataatcgtg tttatatttt ggatgctgct tgaatccaat
     3001 tctctcccca acaatgaggc actggatcac ccactcttgt gacaccacag gcagctgcaa
     3061 tgcttcagca cacttcagca ccgaggctgg gcatgagggg tccgtcacca ccacatcaaa
     3121 tacccctaaa gcaatatctt tgttatgggc acttgaatgg tgctgcttca cagaggctgc
     3181 accaccagtc atgaggatct cagaccagag ctccaggaag ttctgctgtt ggtctgatac
     3241 caagagtacc ttcagattct ggaaaggatt ttcacggggt tgccagtcca gaattctttg
     3301 ctcctcaagg ctgtacccag ctggcaacag ataattacgg tagttctgga gctggttggc
     3361 atggcaacta tcatggaccc agacatgaga cacacaagga atcccactgg caaggcacag
     3421 gaagtacttc cgggttcgac aatgctgatc cgcaattaga agacactggt aagctgtgtt
     3481 acactgggct tcattgaaat cttcaaggat atagccagct cctgctcgaa gctgggattc
     3541 tgtatactgc ttgttgaaag gaggaatttc caaaaattcc tcctcttctt cactgcttcc
     3601 tgtaggacca tctggcagtt tggagcggct ggccaacttg tcactggttg tggccatggt
     3661 aaggagaaat gcgtagccca gaaacaaggt cttgttgaga ggcaaaggcc ctctctgctc
     3721 ttccagggca gagggttcac cggtgttgtc tccactctca caggggctca caaactctcc
     3781 tgcccctact gcaccaggtt ttactgtggc agacttgcga cctcgcttgg caggggaccg
     3841 ttcctcttca gaagtgataa gttttctttt gcctgagaga actcccatgg aggcacgagg
     3901 actttctgtg atctttcggg taggggttgt gctgctgcta ctggaggcag taggggtggc
     3961 tggggagctg acgttactgc gccgtttccg cttcccttcc accaaattgt ctaagctgat
     4021 atctgctgcc tttgtaagag gtgttactgc ttcatagggg ccaagcccat actgctctct
     4081 cagtctgttt ccttgctcca aggacaggat gacagccatt cgcttatacc actttctttg
     4141 gccttctttt tcaatgctgt agtacagttc cccagactcc ttcctatgtc ctttcaccac
     4201 tcctgcactg aaatactcat cctccgagag ggccgtcact tcagtgtcca gcgggatggg
     4261 gtcacataac agaatgtctt tgcccaacac atcacattcg tacccatcat caaagagcaa
     4321 tttatacttc ccagctccga catctcgtgt gattttccca gagtaaaagt agccattgga
     4381 tgaccacttg gctacaacac ggagccctac aaagctattt cctggagagg aggcatctaa
     4441 gccatcagaa gggccagcag cagcctggaa aggaatttct ggagagtcac tacgacgcaa
     4501 agcaccagca cccacatctg ttcgtctggt ggagtctggc actcggggca cgacacggct
     4561 gaaggattta tcatctggtg acaagttagg tgaaatgtcc tctatgccca aggggccagg
     4621 cacagctgtt tctctggttc cagtggtccg agaaggtggg cggccccttc gcccacgccc
     4681 acgaggcgtg actggagcct tccctccctg tctgatgcca aggcctgcat caccatcctc
     4741 ctcacacact ggcgtccctg tctgactgac cccttttcta ggactcagtt ttcctgggcc
     4801 tcctcgggag ctgggtaagg caaaatctgc gggttctgtc ccgctggttt tccctctgag
     4861 tggtccggct cctttccctg agcttccact gctgtgcata gctgagagac ttgtcccact
     4921 tgatgtgcgg tgtaagctgg atgccttgga ggagaaggag ctgatatccc ccaggtcacc
     4981 tgaggagccc ccagtctgtg aaggggaaac ttcagtttca cactcctgac actctacaat
     5041 tggctcttca gtctcctcag ttacttttct ttctacttct gttccatcca cataatacac
     5101 atctgtaatg acacgagtga caagtgtgcg tacttcccgg attgttctca tgtgacgatg
     5161 taagacatgg ccatgtggag gctgaggcat atcaaactct tcttctccct gggcatggag
     5221 cgactctgta tcatccccag gagcactgac tggtttctca ccactccccc tctcagtctg
     5281 aactgtggca tcttgctttc caaagttctg gtccactgta ctggtcccct gctcacacac
     5341 attctttata gtctgggctg ctgctgaaac agtttctggg accctcaagg aacactccat
     5401 ggtttggatt cctatgttat tttgggcggg cctttcaatc aaggccttac taggattttc
     5461 cttattagta ctccgtcctt ctttctggtc ttctagcaca tctgtcacca ttgcctctcc
     5521 ttgaggactg gatggccctt gtatgaccat cttctgggca gcaggagaaa cagggtcctt
     5581 gacaggacta atgggcttca taggctgttg agcctgcctg attgtatggt caccctccaa
     5641 tttttctttc tcctcttctc cttgagcact tggaaagtgg agcaagttcc ccctgatggg
     5701 tgtggtgggg ggatcctcac ttcgagcctc attctcttgc ctggcttcac agatacagct
     5761 caacacagac atggtcttct ggggactggc aacagactca gcaacagcag tagatccatt
     5821 ttttctttca ccagttgcag gcttttccag gttgaactgc aaagactctt cactcgcctc
     5881 agtctcagga ctaaccagtt tcattcttag acataattta tcatccacgt ctggggcagc
     5941 cccagaatcc ccttttccac tcccaagtat gggatcatgg gtctccacca tgctttcaga
     6001 catgacatca ctggttgcta tggtgctttc tggatagttg ctaataccaa taggagtact
     6061 gtgtctcttg ggctccaatt ttaggtgccc aataagaggt ggggttgcac cagtcaatgg
     6121 tgggatgata tcaccttctt taggcaaagt gaaatgaaat ggggtttcac tagaactttc
     6181 acagaagctt tgtgcggcat gggcagaggg cttccccagc ttctgggcct ctgcatctga
     6241 gcttagctgc tttgcattag ccatttttga gtcttctgtt aatgaattac ttgttttctc
     6301 ctgagcttgg ggctgctgca actcctggtc aagtctaagc gggtcgtcag cgcgtactaa
     6361 aggtaaggaa ggctgtgcag aatcttgctc tacaggttct gtttctgcag tccctgattt
     6421 cagatctcct tcatattcta cactcttttc ttccttggtg tctaatctat tctcagcaca
     6481 tggttctatt tctggagcaa tatcctccca tgactgggca tctgagcact tctccactcc
     6541 ctccaaaggt tcacaagaaa catcaactct gggagatggc tctttcacat ctacaatgac
     6601 aacactggag tcctctgaag tagcttcttc ccaagcttcc tgttccttat gttccaattc
     6661 ttggtcaagt attgccaact tttgagggga atcaatacta atcacactgg tttcaacttc
     6721 catagcttct gagcattctt tttttggcat ttccttttga agacacaacc cttgggcctg
     6781 agcttcagtc agagaaaggt gcaacggaac actctccata ttttcttctt tgagttcctc
     6841 tccttgagct tcacaaggtg tctcagggat ttcttccacc tcagaccctg aagacccctc
     6901 ctctggatgg tgttctttaa tttccatagc ttcctcctga tctaacacac tagaaagtgc
     6961 ctcagatcga gtagctggtg acggaactgc ctgagccccc gaatcacaag tgagatcaat
     7021 acaaacatct tctgctaccg tttcttctct gcccttgcaa ccagtggcta aaatactaat
     7081 gtcatccctg gtgtctgtat catctccctt tgttttgtca tcattctgag ccagttgtac
     7141 ttcaccatcc tgtgctggat tcatcaggat actatcattt tcagcaggaa caaatttaga
     7201 attgagaact ggagacatgg gttccgtatc ctcaatctgt gcgttttctc catcttcatc
     7261 aattctgtga gaactcaagc tctccatctt tggggactga gcatattcac ttgtagaaag
     7321 catcaacttg caagaatccc ctgtcaaaga tagcccaaga tcctcagggg aattctttgg
     7381 ttcaatctct gaagttttag aacactcaac tgtcaaagat gaactatgca tatctccatc
     7441 tttcttccca tcatttgatt gttcttccag acttggggaa ggaataaaaa tgtcatgaga
     7501 aaactgaggc tgggctggga taggaagtga cccaggaggg aagactggtg tgctctgagc
     7561 tattggtgtt gaggcttgtg gtgatacagt ggactcaggc aggagagggg ggttttctaa
     7621 ctccactggt tcaccacttt gaagtttctt ctgaaaaggc tctcctcctt cttcagataa
     7681 cactgacgtg tccattggct tatcttgtct cccttcttgc tctgtgggac tgctaggaac
     7741 gataaaagga gtagatcgga aagcatcagg agaaggagca acaagatctg aagaattcgt
     7801 ggaaagactg tcctgaacaa gggacctctg accagagagc tgcaggagat gcagagtggt
     7861 ggcaggagtg gaagccaaag aacacccacc ttcctccctt gaaggagtag agcaaccatc
     7921 agaagatact gttttattgc tctggtcaaa caagtcttcc tgagctgaca aaacctcagg
     7981 ctctggtgac ttctgaatct gcagtccact ttccataagt tcttgtgcag acaactgttc
     8041 ttttgcttcc atagcagcaa cagatgcttt ggggctaaaa ggcatgtcct ctgaccttgc
     8101 aggtggtgga ttttgctctt ttacaacatg tacatcctta ctgggctgtg ctgtcacagg
     8161 gatgtccttg ctggactgtt ctgctatggg gatatcttcg ttggactgtt cttcatgctt
     8221 aattgcagta ttagcatcca catcagacag cctggtataa ccagagttgg tggttactga
     8281 ttgtagctgc tctttgtcca cttcatatgg cacagtattt tcctcaacat cctgagcctg
     8341 ggcgagttcc agaaccccaa aacccaactg cgctgaggca gtatcttcag caccaaggga
     8401 atgtgtagta ttgcctgaag tatcttcttc cttctctttc tccttctgtt ccaactcttc
     8461 tcccttctct tcctccacag caggagcaga ttccactgac attcccagaa cactgcttgt
     8521 cctgtttggc tgaggtaact gctcaatgac ctgagcgatg gaaccacatg tgtccaagtt
     8581 agaagaatcc acagggtctg caaccttgtt ttctttcaaa tgttcattga acccactatt
     8641 accgtctcct cgttcttctc cagctgtttg ttcaggattg gacacaacat ccaacacagg
     8701 attttctttg tgcgtctgga gattaggaag gtgtcgagat agcatactga agtgagaacc
     8761 agaatcatcc tctagaacct gcgcttcagg ctgagcatct tcaattatca ggcaaggagt
     8821 atcttgctga gcgaaatctg aatccaactg agctccagta gggtccatga agcctgcttt
     8881 tttgtacaaa cttgtcttgt catcgtcatc cttgtaatcg atgtcatgat catatgtatc
     8941 accgtcatgg tctttgtagt ccatggtgga agcttaagtt taaacgctag agtccggagg
     9001 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg
     9061 atctgacggt tcactaaacg agctcgtcga cgatctctat cactgatagg gagatctcta
     9121 tcactgatag ggagagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     9181 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     9241 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     9301 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     9361 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     9421 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     9481 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     9541 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     9601 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     9661 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
     9721 cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc
     9781 ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac
     9841 tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag
     9901 cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag
     9961 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10021 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    10081 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    10141 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    10201 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10261 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10321 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10381 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10441 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10501 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10561 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10621 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10681 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10741 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10801 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10861 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10921 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10981 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11041 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11101 aaaagatcaa aggatcttc
//
