LOCUS       sequence-439811-        4991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for inserting 7xGFP11-HA at the end of Drosophila 
            5-HT2BR coding frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM
  TITLE     Serotonin acts through multiple cellular targets during an olfactory
            critical period.
  JOURNAL   bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413.
  PUBMED    38645269
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413."
COMMENT     Sequence Label: pTwist Amp_d5-HT2BR-7xsfGFP11-HA-HDR-donor
FEATURES             Location/Qualifiers
     source          1..4991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(52..99)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(133..180)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     primer_bind     complement(1317..1334)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1317..1333)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1326..1348)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1447..1551
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1552..1620,1621..2412)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSATITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1770..1789)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    2512..2536
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      2653..3241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3142..3161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3394..3416
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3413..3429
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3413..3429
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(4698..4724)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(4728..4775)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(4791..4838)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(4854..4901)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(4917..4964)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(join(4980..4991,1..36))
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 agcatttaca tactcatgaa ggaccatgtg gtcacggccg ccactaccgc tagtgatgcc
       61 ggcggcgttc acgtactcgt gcagcaccat gtggtcccgg ctgcctcctc cgctgcctcc
      121 gtcgcctccg ctagtgatgc cggcggcgtt cacgtactcg tgcagcacca tgtggtcccg
      181 gctgcctcct ccgctgcctc cgtcgcctcc gcttcctcca tcgccgagtc tgctcggtcg
      241 ccaggcactc gtgctcgaat agcgacacag caggaccttc ttgaacgcct gccggaacac
      301 cttgttgaag atggtgtaga agatggggtt caccatggag ctagcgtagc ccagccaggt
      361 gaccacgtcg aagacccagt ggctaattcg ttcctcgcac tccgcgcaca ccgtgggcag
      421 gagattgagg acgaagaatg gcgaccagag gattacgaac gtaaagaaca ccactcccag
      481 caccttggtg gccttctgct ccagcctgat gatcctgccg tggcgcgagg agtttctgga
      541 gatcacgctg gagttccgcg agtgatgaga tcgcaccgtc tgctgacgct gcaacatgca
      601 gggtgaggat gttgtcgtcc tggtcttgcc accacattcg cccgctccct ggtagttcat
      661 attctggtga ctcctcaagg tggccgatct tctcaacggt gtggatgagt tggaggtggt
      721 cgctgttttg gtgggcgtca acagcaggga tgttcgcact ccgccaaaac tgctgccccg
      781 ccgacgatga cgacttgaat cccacgtgac caccgaggcg gtctttgaga tgctcaactt
      841 tcgtcggcca ggacaaggag ttcccggagc agccaagtac ccactaccat cgctctgaac
      901 tgtcagcgag ctgccctgat tgtgagcaga gtgtggactg agggtggagc caactgtggc
      961 tggagccgct cctgccggtc cacctgttat gccggcactc acactggagg tcaatgactt
     1021 tttattgcca ctactgcaca tgagtagctc catctcgctg ggcgaactgc tcaccgccgt
     1081 cgtggtggtg gtcactcgga aggcggacga aataatcttc acctccgccg atttcacaca
     1141 attctggagc ggtctgtccc caaaatacgg acaagtgcaa accgagggca gctgcaggta
     1201 gtccgactga tgattctgcc cggctatgtg gcactgcttc gccttctgcg gaggatctag
     1261 cataattagg tcaggcggaa tggcacttcc ttccagatgc gtgcggatta tgcatgactg
     1321 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg cttgtggtgg atcacagtta
     1381 ttggagactt ccttggccgt acgatgggtt ttgtttcagg tatctggcac ttttcgggga
     1441 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1501 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1561 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1621 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1681 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1741 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1801 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1861 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1921 accataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1981 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2041 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2101 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2161 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2221 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggctc tcgcggtatc
     2281 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2341 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2401 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2461 catttttaat ttaaaaggat ctaggtgaag atccttgaat tgccattatc aacaagtttg
     2521 tacaaaaaag caggctccga attggtatca cgaggacgat ttgaagcccc ctacccattt
     2581 acgctgactt gacgggacgg cggctttgtt gaatagagcg tcagaccccg tagaaaagat
     2641 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2701 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2761 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     2821 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2881 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2941 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3001 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3061 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3121 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3181 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3241 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3301 gttctttcca gcgacatatg ccatacgccg aactgaactc cacctaatgt cttggagatt
     3361 gtcggatagc gcggaatagg attgtgccct ctgagcggat aacaatttca cacaggaaac
     3421 agctatgacc atgacacatg catccaccat cgcagactta tcatcactga gcctccacct
     3481 agcctataat tgcggccgca gcacattcga aactttcatc cggctttcgt tattacagtt
     3541 ggccccttgt tgttgtttga tgtccttcag ccagctgctc cgacttggta ttcgatttgg
     3601 ccagcaggct tctggctgcc ttctggccac cggattctcc attcacatcc tgaccgtcaa
     3661 attcttacgt gaccagctga aatttttcat ttggccacag aaataaaaca aaatgaatga
     3721 acttttcaaa taaaaatagc aatctgccgg tccaaccaca caaacaaaca acaaaagtca
     3781 caaattgccg tggatttgtg tacgtgtcgg tttctttaat ggataccgat gaaattcttt
     3841 gtgcattggc gggcagtgaa tttgccataa atttaatgca taattgccaa atgccaaacc
     3901 aaatagcgtt tatcgatcga agtttgcgaa gcgacattgg cgctttattt ttaagaattt
     3961 caaaagttga actttatata tacaatcttt aaatttacgt gaattttcaa gtttattttc
     4021 gagtacgtag tgaatctaaa aggaatttcg cggaaaatgc tatcaaatat caacagattg
     4081 acatgaactt tcggtgagcc agaactttgt cgataactga agagcgttgc tataaagtaa
     4141 atgtgtttgc cggtattacg ttgctcgaaa gcaaacattc cgcaattaag tgcatcttca
     4201 ccgtttgctg agagctgagg gaaatggaag gaaccaccag gggtgagtag catgctcgtt
     4261 atgctgttgt ctgtggctag tttaatcccc gacccttggc aatcctgcgg ggatccgact
     4321 ccacttcaat atctaattca cttggcccca cttcttacgc ctgggacatg tgttttcaat
     4381 ggcatgcaaa ttgaatttga aattaattag aggcacataa gaatttaagc cccaaggaca
     4441 cacgtgtgag cagtgcggag tgcgtggtgt ggagtgcggg tgggcattat cgtcctttcg
     4501 agattgcgta tacgacacgt tgtgcggtgg accgcaggcg aaatgaccgg cctttgccgt
     4561 atttatatgt cacttgccac tgccacactt cttttcagaa cgccccgcac cggagcagtg
     4621 gaatcctgaa aatccacact ggcgcacttg tgggtattcg tcttcagttt gcccggttta
     4681 acggtcgtct gttattaagc gtaatctgga acatcgtatg ggtaactagt aatgccagct
     4741 gcgtttacgt attcgtgaag caccatatgg tctcgtccgc ccgacccacc agttataccc
     4801 gccgcgttca catactcatg cagcaccatg tgatcccggc ctccggaccc tccggttatt
     4861 ccggctgcat tgacatattc atgtagtacc atatggtcgc gtcctccact accgccagtg
     4921 atgcctgcgg cgttaacgta ttcgtggaga accatatgat ctctacctcc agagccacct
     4981 gtaatcccag c
//
