LOCUS       sequence-439813-       13845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the human receptor LRRC37B in mammalian cells together 
            with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13845)
  AUTHORS   Libe-Philippot B, Lejeune A, Wierda K, Louros N, Erkol E, Vlaeminck 
            I, Beckers S, Gaspariunaite V, Bilheu A, Konstantoulea K, Nyitrai H,
            De Vleeschouwer M, Vennekens KM, Vidal N, Bird TW, Soto DC, Jaspers 
            T, Dewilde M, Dennis MY, Rousseau F, Comoletti D, Schymkowitz J, 
            Theys T, de Wit J, Vanderhaeghen P
  TITLE     LRRC37B is a human modifier of voltage-gated sodium channels and 
            axon excitability in cortical neurons.
  JOURNAL   Cell. 2023 Dec 21;186(26):5766-5783.e25. doi: 
            10.1016/j.cell.2023.11.028.
  PUBMED    38134874
REFERENCE   2  (bases 1 to 13845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11"; journalName: "Cell"; date: "2023-12-21- 
            21"; volume: "186"; issue: "26"; pages: "5766-5783.e25"
COMMENT     Sequence Label: pCIG-LRRC37B
FEATURES             Location/Qualifiers
     source          1..13845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2602..2905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2907..3182
                     /label=chicken beta-actin promoter
     intron          3183..4199
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4123..4145
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4207..4226
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4262..4281
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4262..4280
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4295..4313
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4295..4312
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(7207..7781,7782..7784,7785..7793)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7374..7391)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7601..7620
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7794..7796,7797..7799,7800..8513)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7794..7796,7797..7799,7800..8510)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7839..7860)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8100..8119)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8447..8468
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8537..9125
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8590..8610)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9008..9019)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9128..9144)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9129..9145)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9650..9830
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9856..9873)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9862..10086
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10132..10560
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10219..10238)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10429..10450
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10569..10589)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10574..10903
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10754..10889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10816..10835
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10951..10998
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11017..11391
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11521..11642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11558..11577)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11612..11631
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11691..11707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11691..11707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11704..11726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11715..11731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11739..11745,11746..11763,11764..11769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11784..11805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11922..11939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12093..12681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12173..12192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12852..13643,13644..13712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13475..13494
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13713..13817)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2641 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2821 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2881 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     2941 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     3001 agcgatgggg gcgggggggg ggggggggcg cgccaggcgg ggcggggcgg ggcgaggggc
     3061 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     3121 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     3181 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     3241 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     3301 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     3361 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     3421 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     3481 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     3541 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     3601 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     3661 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     3721 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     3781 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     3841 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     3901 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     3961 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     4021 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     4081 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     4141 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     4201 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgctcgagc
     4261 gtaatacgac tcactatagg gagacccaag cttgatttag gtgacactat agaatacaag
     4321 ctacttgttc tttttgcagg atcggttacc atgtcttggc tgcgtttctg gggcccatgg
     4381 cccctcctta cgtggcaact attgtcttta ctagtcaagg aggctcagcc tctggtgtgg
     4441 gtcaaggacc cgctccagct gacctctaac cccctggggc cacctgagcc ctggtcttcc
     4501 cgctcctccc atctcccatg ggaatctccc catgcacctg ctcccccagc agccccgggg
     4561 gactttgatt acctggggcc ctctgcttct tcgcagatgt cagccctgcc tcaggaacca
     4621 actgaaaatt tggctccatt cctgaaggaa ttggattcag ctggagagct gcccctgggg
     4681 ccagagccgt tcttggctgc acatcaggac ttaaatgaca agcggactcc agaagaaagg
     4741 ctcccagagg tggttccgct tctcaaccgg gatcagaacc aggccctagt tcagcttcct
     4801 cgcctcaagt gggttcaaac tacagatcta gatcgggctg caggtcatca ggcagatgaa
     4861 atacttgttc cactagacag taaggtttca agaccaacca aatttgttgt ttcgcccaag
     4921 aacctgaaga aagatctagc tgaacgttgg agccttcctg agattgttgg gattccacac
     4981 caattatcca aacctcagcg tcagaaacag actttgccag atgattattt gagtatggac
     5041 acactgtatc ccggcagcct acctccagaa ctccgggtga acgcagatga gcctccaggg
     5101 cctcctgagc aagttggact ttctcaattc catctagagc ccaaaagtca aaatccagag
     5161 acccttgaag acatccagtc ctcttcactc caggaagaag ccccagcgca gcttctacag
     5221 ctccctcagg aggtagaacc ttcaacccag caggaggccc cagctctgcc tccagagtcc
     5281 tctatggaga gtctagctca aactccactg aatcatgaag tgacagttca acctccaggt
     5341 gaggatcaag ctcattataa tttgcccaag tttacagtca aacctgcaga tgtggaggtt
     5401 accatgactt cagagcctaa aaatgagaca gaatctaccc aagcccagca ggaggcccca
     5461 attcagcctc ccgaggaggc ggaaccttct tctacagccc tgaggactac agatcctcct
     5521 ccagaacacc ctgaggtgac acttccacct tcagacaagg gtcaggctca gcattcacac
     5581 ctgactgaag ccacagttca acctctggac ctggagctta gcataactac agagcctact
     5641 acagaggtta aaccgtctcc aaccacggag gaaacctcag ctcagcctcc agacccgggg
     5701 cttgccataa ctccagaacc cactacagag attggacatt ccacagccct ggagaagact
     5761 agagctcctc atccagacca ggttcagact ctgcatcgaa gcctgactga agtcacaggt
     5821 ccacctacaa agttagaatc ttcgcaggat tcattggtgc agtctgaaac tgcaccagag
     5881 gaacagaagg cctccacaag caccaacata tgtgagctct gcacctgcgg agatgagact
     5941 ctgtcatgtg ttggtctcag cccaaagcag aggctccgcc aagtgcctgt gccagagccc
     6001 gacacctaca atggcatctt caccacctta aatttccaag gaaactatat ttcatacctt
     6061 gatggaaatg tatggaaagc atacagttgg accgagaaac taattctcag tgaaaattat
     6121 ttgactgaat tacctaagga ttcatttgaa ggcctgctat acctccagta tttagattta
     6181 tcctgcaata aaatacgata tattgaaaga caaacatttg aatcactacc atttttgcag
     6241 tatataaatc tgggctgcaa tttaattaca aaactgagcc ttggaacatt tcaggcctgg
     6301 cacggaatgc agtttttaca caacttaatt ctcaatcgca atcctctgac tactgtcgaa
     6361 gatccatatc tctttgaact gccggcatta aaatatctag acatgggaac aacacacatc
     6421 acacttacaa cacttaagaa cattctcacg atgactgttg aactggaaaa actgatctta
     6481 cctagccata tggcctgctg cctctgccaa tttaaaaata gcattgaggc tgtctgcaag
     6541 acagtcaagc tgcattgcaa cactgcatgt ctgactaaca gcatacattg tcctgaagaa
     6601 gcatctgtag ggaatccaga aggagcgttc atgaagatgt tacaagcccg gaagcagcac
     6661 atgagcactc agctgactat tgagtcggag gcgccctcag acagcagtgg catcaacttg
     6721 tcaggctttg ggggtgatca gcttgaaatt cagctaaccg agcagctacg gtccctcatc
     6781 cccaacgagg atgtgagaaa gttcatgtct catgttatcc ggaccttgaa aatggaatgt
     6841 tcagaaacac atgtgcaagg gagctgtgcc aagctcatgt tgcgaacagg cctcctgatg
     6901 aagcttctca gcgagcagca ggaagcaaag gcattgaatg tagaatggga tacggaccaa
     6961 caaaaaacaa attatattaa tgagaacatg gaacagaatg aacagaaaga gcagaagtca
     7021 agtgagctca tgaaagaagt tccaggagat gactataaga acaaactcat cttcgcaata
     7081 tctgtgactg taatactaat aattttgatt ataatttttt gtcttataga ggtgaattca
     7141 cataaaaggg catcagaaaa atacaaagac aacccatcaa tatcaggagc ctaggttaac
     7201 gatccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg
     7261 ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag
     7321 ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc
     7381 caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg
     7441 aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag
     7501 gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca
     7561 gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt
     7621 caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc
     7681 tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa
     7741 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga
     7801 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     7861 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     7921 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     7981 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     8041 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     8101 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     8161 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     8221 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     8281 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     8341 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     8401 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     8461 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taaaattcga
     8521 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     8581 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     8641 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     8701 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     8761 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     8821 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     8881 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     8941 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     9001 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     9061 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     9121 cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa
     9181 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     9241 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     9301 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     9361 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     9421 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     9481 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     9541 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     9601 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     9661 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9721 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     9781 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     9841 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     9901 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     9961 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
    10021 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
    10081 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
    10141 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    10201 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    10261 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    10321 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    10381 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    10441 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    10501 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    10561 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    10621 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    10681 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    10741 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    10801 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
    10861 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
    10921 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
    10981 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
    11041 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
    11101 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
    11161 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
    11221 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
    11281 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
    11341 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
    11401 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
    11461 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
    11521 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    11581 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11641 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
    11701 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    11761 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    11821 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    11881 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    11941 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    12001 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    12061 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    12121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    12181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    12241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    12301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    12481 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    12541 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12601 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12661 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    12721 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12781 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    12841 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12901 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    12961 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    13021 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    13081 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    13141 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    13201 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    13261 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    13321 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    13381 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    13441 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    13501 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    13561 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13621 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13681 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    13741 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13801 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
