LOCUS       sequence-439815-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Construct for labelling cell nuclei in mammalian cells using Histon 
            protein 2B fused to mScarlet3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Gadella TWJ Jr, van Weeren L, Stouthamer J, Hink MA, Wolters AHG, 
            Giepmans BNG, Aumonier S, Dupuy J, Royant A
  TITLE     mScarlet3: a brilliant and fast-maturing red fluorescent protein.
  JOURNAL   Nat Methods. 2023 Apr;20(4):541-545. doi: 
            10.1038/s41592-023-01809-y. Epub 2023 Mar 27.
  PUBMED    36973546
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-023-01809-y"; journalName: "Nat Methods"; date: 
            "2023-04"; volume: "20"; issue: "4"; pages: "541-545"
COMMENT     Sequence Label: pCS2FA-H2B-mScarlet3
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..384
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             391..1077
                     /codon_start=1
                     /product="bright cysteine-free monomeric red fluorescent 
                     protein with fast and complete maturation (Gadella et al., 
                     2023)"
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     primer_bind     789..808
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggccatgc cagagccagc gaagtctgct cccgccccga aaaagggctc caagaaggcg
       61 gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa ggagagctat
      121 tccatctatg tgtacaaggt tctgaagcag gtccaccctg acaccggcat ttcgtccaag
      181 gccatgggca tcatgaattc gtttgtgaac gacattttcg agcgcatcgc aggtgaggct
      241 tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat ccagacggcc
      301 gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg tactaaggcc
      361 atcaccaagt acaccagcgc taaggatctc atggatagca ccgaggcagt gatcaaggag
      421 ttcatgcggt tcaaggtgca catggagggc tccatgaacg gccacgagtt cgagatcgag
      481 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgag ggtgaccaag
      541 ggtggccccc tgcccttctc ctgggacatc ctgtcccctc agttcatgta cggctccagg
      601 gccttcacga agcaccccgc cgacatcccc gactactgga agcagtcctt ccccgagggc
      661 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg ccgtgtccgt ggcccaggac
      721 acctccctgg aggacggcac cctgatctac aaggtgaagc tccgcggcac caacttccct
      781 cctgacggcc ccgtaatgca gaagaagaca atgggctggg aagcatccac cgagcggttg
      841 taccccgagg acgtcgtgct gaagggcgac attaagatgg ccctgcgcct gaaggacggc
      901 ggccgctacc tggcggactt caagaccacc tacagggcca agaagcccgt gcagatgccc
      961 ggcgccttca acatcgaccg caagttggac atcacatccc acaacgagga ctacaccgtg
     1021 gtggaacagt acgaacgctc cgtggcccgc cactccaccg gcggctccgg tggctcctaa
//
