LOCUS       sequence-439817-       13927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the chimpanzee receptor LRRC37B in mammalian cells 
            together with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13927)
  AUTHORS   Libe-Philippot B, Lejeune A, Wierda K, Louros N, Erkol E, Vlaeminck 
            I, Beckers S, Gaspariunaite V, Bilheu A, Konstantoulea K, Nyitrai H,
            De Vleeschouwer M, Vennekens KM, Vidal N, Bird TW, Soto DC, Jaspers 
            T, Dewilde M, Dennis MY, Rousseau F, Comoletti D, Schymkowitz J, 
            Theys T, de Wit J, Vanderhaeghen P
  TITLE     LRRC37B is a human modifier of voltage-gated sodium channels and 
            axon excitability in cortical neurons.
  JOURNAL   Cell. 2023 Dec 21;186(26):5766-5783.e25. doi: 
            10.1016/j.cell.2023.11.028.
  PUBMED    38134874
REFERENCE   2  (bases 1 to 13927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11"; journalName: "Cell"; date: "2023-12-21- 
            21"; volume: "186"; issue: "26"; pages: "5766-5783.e25"
COMMENT     Sequence Label: pCIG-LRRC37Bchimpanzee-HA
FEATURES             Location/Qualifiers
     source          1..13927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        109..412
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        414..687
                     /label=chicken beta-actin promoter
     intron          688..1704
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1628..1650
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1712..1731
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1767..1786
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1767..1785
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1800..1818
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1800..1817
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             4738..4764
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(4796..5370,5371..5373,5374..5382)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4963..4980)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5190..5209
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5383..5385,5386..5388,5389..6102)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5383..5385,5386..5388,5389..6099)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5428..5449)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5689..5708)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6036..6057
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6126..6714
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6179..6199)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6597..6608)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6717..6733)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6718..6734)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7239..7419
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7445..7462)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7451..7675
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7721..8149
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7808..7827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8018..8039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8158..8178)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8163..8492
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8343..8478
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8405..8424
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8540..8587
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8606..8980
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9110..9231
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9147..9166)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9201..9220
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9280..9296)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9280..9296)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9293..9315)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9304..9320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9328..9334,9335..9352,9353..9358))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9373..9394
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9511..9528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9682..10270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9762..9781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10441..11232,11233..11301))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11064..11083
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11302..11406)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11481..11500)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11672..12051
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12053..12251
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12206..12226
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             12269..12449
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12496..12621
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13114..13347
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13532..13576
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(13874..13927,1..64)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
       61 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaatccgcg ttacataact
      121 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      181 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      241 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      301 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      361 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
      421 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
      481 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggcgcgcca
      541 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
      601 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
      661 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
      721 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
      781 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
      841 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
      901 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
      961 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     1021 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     1081 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     1141 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     1201 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     1261 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     1321 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     1381 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
     1441 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
     1501 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     1561 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     1621 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     1681 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     1741 cattttggca aagaattgct cgagcgtaat acgactcact atagggagac ccaagcttga
     1801 tttaggtgac actatagaat acaagctact tgttcttttt gcaggatcct cactataggg
     1861 agacccaagc tggctagcat gtcttggctg cgtttctggg gcccatggcc cctccttacg
     1921 tggcaactat tgtctttact agtcaaggag gctcagcctc tggtgtgggt caaggacccg
     1981 ctccagctga cctctaaccc cctggggcca cctaagccct ggtcttcccg ctcctcccat
     2041 ctcccgtggg aatctcccca tgcacctgct cccccagcag ccccggggga ctttgattac
     2101 ctggggccct ctgcttcttc gcagatgtca gccctgcctc aggaaccaac tgaaaatttg
     2161 gctccattcc tgatggaatt ggattcagct ggagagctgc ccctggggcc agagccgttc
     2221 ttggctgcac atcaggattt aaatgaaaag cggactccag aagaaaggct cccagaggtg
     2281 gtgccgcttc tggaccggga tcagaaccag gccctagttc agcttcctcg cctcaagtgg
     2341 gttcaaacta cagatccaga tcgggctgca ggtcatcagg cagatgaaat acttgttcca
     2401 ctagacagta aggtttcaag accaaccaaa tttgttgttt cgcccaagaa cctgaagaaa
     2461 gatctagctg aacgttggag ccttgctgag attgttggga ttccacacca attatccaaa
     2521 cctcagcgtc agaaacagac tttgcctgat gattatttga gtatggacac actgtatccc
     2581 ggcagcctgc ctccagaact ccgggtgaac gcagatgagc ctccagggcc tcctgagcaa
     2641 gttggacttt ctcaattcca tctagagccc aaaagtcaaa atccagagac ccttgaagac
     2701 atccagtctt cttcactcca ggaagaagcc ccagcgcagc ttctacagct ccctcaggag
     2761 gtagaacctt caacccagca ggaggcccca gctctgcctc cagagtcctc tatggagagt
     2821 ctagctcaaa ctccactgaa tcatgaagtg acagttcaac ctccaggtga ggatcaagct
     2881 cattataatt tgcccaagtt tacagtcaaa cctgcagatg tggaggttac catgacttca
     2941 gagcctaaaa atgagacaga atctacccaa gcccagcagg aggccccaat tcagcctcca
     3001 gaggaggcgg aaccttcttc tacagccctg aggactacag ctcctcctcc agaacaccct
     3061 gaggtgacac ttccaccttc agacaagggt caggctcagc attcacacct gactgaagcc
     3121 acagttcaac ctctggacct ggagcttagc ataactacag agcctactac agaggttaaa
     3181 ccgtctccaa ccacggagga aacctcagct cagcctccag acctggggct tgccataact
     3241 ccggaaccca ctacagagat tggacattcc acagccctgg agaagactag agctcctcat
     3301 ccagaccagg ttcagactct gcatcgaagc ctgactgaag tcacaggtcc acctacaaag
     3361 ttagaatctt cgcaggattc attggtgcag tctgaaactg caccagagga acagaaggcc
     3421 tccacaagca ccaacatatg tgagctctgc acctgcggag atgagactct gtcatgtgtt
     3481 ggtctcagcc caaagcagag gctcagccaa gtgcctgtgc cagagcccga cacctacaat
     3541 ggcatcttca ccatcttaaa tttccaagga aactatattt catacattga tggaaatgta
     3601 tggaaagcat acagttggac cgagaaacta attctcagtg aaaattattt gactgaatta
     3661 cctaaggatt catttgaagg cctgctatac ctccagtatt tagatttatc ctgcaataaa
     3721 atacgatata ttgaaagaca aacatttgaa tcactaccat ttttgcagta tataaatctg
     3781 ggctgcaatt taattacaaa actgagcctt ggaacatttc aggcctggca cggaatgcag
     3841 tttttacaca acttaattct caatcgcaat cctctgacta ctgtcgaaga tccatatctc
     3901 tttgaactgc cggcattaaa atatctagac atgggaacaa cacacatcac acttacaaca
     3961 cttaagaaca ttctcacgat aactgttgaa ctggaaaaac tgatcttacc tagccatatg
     4021 gtctgctgcc tctgccaatt taaaaatagc attgaggctg tctccaagac agtcaagctg
     4081 cattgcaaca ctgcatgtct gactaacagc atacattgtc ctgaagaagc atctgtaggc
     4141 aatccagaag gagcgttcat gaagatgtta caagcccgga agcagcacat gagcactgag
     4201 ctgactattg agtcggaggc gccctcagac agcagtggca tcaacttgtc aggctttggg
     4261 agtgatcagc ttgaaattca gctaaccgag cagctacggt ccctcatccc caacgaggat
     4321 gtgagaaagt tcatgtctca tgttatccgg accttgaaaa tggaatgttc agaaacacat
     4381 gtgcaaggga gctgtgccaa gctcatgttg caaacaggcc tcctgatgaa gcttctcagc
     4441 gagcagcagg aagcaaaggc attgaatgta gaatgggata cggaccaaca aaaaacaaat
     4501 tatattaatg agaacatgga acagaatgaa cagaaagagc agaagtcaag tgagctcatg
     4561 aaagaagttc caggatatga ctataagaac aaactcatct tcgcaatatc tgtgactgta
     4621 atactaataa ttttgattat aattttttgt cttatagagg tgaatgcaca taaaagggca
     4681 tcagaaaaat acaaagacaa cccatcaata tcaggaggcc tcgagtctag agggccctac
     4741 ccatacgatg ttccagatta cgcttgataa acccgctgat cagcctcgag atccgcccct
     4801 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     4861 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     4921 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     4981 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5041 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5101 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5161 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5221 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtacacat
     5281 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5341 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag
     5401 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5461 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5521 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     5581 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     5641 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5701 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5761 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5821 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     5881 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     5941 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     6001 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     6061 gccgccggga tcactctcgg catggacgag ctgtacaagt aaaattcgat atcaagctta
     6121 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6181 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6241 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6301 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6361 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6421 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6481 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6541 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6601 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6661 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     6721 accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6781 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     6841 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6901 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     6961 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     7021 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     7081 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     7141 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     7201 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     7261 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7321 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7381 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     7441 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7501 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7561 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     7621 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     7681 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     7741 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7801 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7861 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7921 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7981 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8041 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8101 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     8161 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8221 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     8281 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     8341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8401 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     8461 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     8521 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     8581 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     8641 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     8701 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     8761 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     8821 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     8881 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     8941 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     9001 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     9061 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     9121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9241 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9301 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9361 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9421 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9481 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9541 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9601 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9661 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9721 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9781 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9841 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9901 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9961 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10021 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10081 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    10141 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10201 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10261 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10321 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10381 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10441 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10501 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10561 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10621 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10681 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10741 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10801 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10861 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10921 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    10981 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11041 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11101 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11161 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11221 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11281 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11341 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11401 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
    11461 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
    11521 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
    11581 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
    11641 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
    11701 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
    11761 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
    11821 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
    11881 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
    11941 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
    12001 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
    12061 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
    12121 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
    12181 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
    12241 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
    12301 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    12361 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    12421 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
    12481 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
    12541 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
    12601 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
    12661 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
    12721 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
    12781 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
    12841 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
    12901 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
    12961 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
    13021 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    13081 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    13141 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    13201 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    13261 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    13321 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    13381 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    13441 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    13501 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    13561 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    13621 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    13681 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    13741 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    13801 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
    13861 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    13921 tagtaga
//
