LOCUS       sequence-439853-        8965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            PRDM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8965)
  AUTHORS   Pierson Smela M, Kramme CC, Fortuna PRJ, Wolf B, Srikar Kavirayuni 
            V, Adams J, Ma C, Velychko S, Widocki U, Goel S, Chen T, Vincoff S, 
            Dong E, Kohman RE, Kobayashi M, Shioda T, Church GM, Chatterjee P
  TITLE     Rapid Human Oogonia-like Cell Specification via Combinatorial 
            Transcription Factor-Directed Differentiation
  JOURNAL   bioRxiv 2022.07.11.499564
REFERENCE   2  (bases 1 to 8965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.11.499564"
COMMENT     Sequence Label: pPB-cT3G-cERP2-PRDM1
FEATURES             Location/Qualifiers
     source          1..8965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    52..70
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     277..301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    367..391
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2879..2903)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2905..2931
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3098..3232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3504..3538
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3549..3566)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3549..3565)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3558..3580)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3774..3793)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3895..3917
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3956..3974)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4042..4146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4147..4215,4216..5007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4365..4384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5178..5766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5667..5686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5920..5937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6143..6164
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6179..6184,6185..6202,6203..6209)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6217..6233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6222..6244
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6241..6257
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6241..6257
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   6283..6345
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6521..6602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7319..7375)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7385..8128)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(8129..8152)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      8152..8161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8181..8392)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        8393..8772
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcacctcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
       61 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
      121 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
      181 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
      241 gtgatagaga acgtataagc ttgcctatat aagcagagct cgtttagtga accgtcagat
      301 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      361 cctaccacaa gtttgtacaa aaaagcaggc ttagccacca tgttggatat ttgcttggaa
      421 aaacgtgtgg gtacgacctt ggctgccccc aagtgtaact ccagcactgt gaggtttcag
      481 ggattggcag aggggaccaa ggggaccatg aaaatggaca tggaggatgc ggatatgact
      541 ctgtggacag aggctgagtt tgaagagaag tgtacataca ttgtgaacga ccacccctgg
      601 gattctggtg ctgatggcgg tacttcggtt caggcggagg catccttacc aaggaatctg
      661 cttttcaagt atgccaccaa cagtgaagag gttattggag tgatgagtaa agaatacata
      721 ccaaagggca cacgttttgg acccctaata ggtgaaatct acaccaatga cacagttcct
      781 aagaacgcca acaggaaata tttttggagg atctattcca gaggggagct tcaccacttc
      841 attgacggct ttaatgaaga gaaaagcaac tggatgcgct atgtgaatcc agcacactct
      901 ccccgggagc aaaacctggc tgcgtgtcag aacgggatga acatctactt ctacaccatt
      961 aagcccatcc ctgccaacca ggaacttctt gtgtggtatt gtcgggactt tgcagaaagg
     1021 cttcactacc cttatcccgg agagctgaca atgatgaatc tcacacaaac acagagcagt
     1081 ctaaagcaac cgagcactga gaaaaatgaa ctctgcccaa agaatgtccc aaagagagag
     1141 tacagcgtga aagaaatcct aaaattggac tccaacccct ccaaaggaaa ggacctctac
     1201 cgttctaaca tttcacccct cacatcagaa aaggacctcg atgactttag aagacgtggg
     1261 agccccgaaa tgcccttcta ccctcgggtc gtttacccca tccgggcccc tctgccagaa
     1321 gactttttga aagcttccct ggcctacggg atcgagagac ccacgtacat cactcgctcc
     1381 cccattccat cctccaccac tccaagcccc tctgcaagaa gcagccccga ccaaagcctc
     1441 aagagctcca gccctcacag cagccctggg aatacggtgt cccctgtggg ccccggctct
     1501 caagagcacc gggactccta cgcttacttg aacgcgtcct acggcacgga aggtttgggc
     1561 tcctaccctg gctacgcacc cctgccccac ctcccgccag ctttcatccc ctcgtacaac
     1621 gctcactacc ccaagttcct cttgcccccc tacggcatga attgtaatgg cctgagcgct
     1681 gtgagcagca tgaatggcat caacaacttt ggcctcttcc cgaggctgtg ccctgtctac
     1741 agcaatctcc tcggtggggg cagcctgccc caccccatgc tcaaccccac ttctctcccg
     1801 agctcgctgc cctcagatgg agcccggagg ttgctccagc cggagcatcc cagggaggtg
     1861 cttgtcccgg cgccccacag tgccttctcc tttaccgggg ccgccgccag catgaaggac
     1921 aaggcctgta gccccacaag cgggtctccc acggcgggaa cagccgccac ggcagaacat
     1981 gtggtgcagc ccaaagctac ctcagcagcg atggcagccc ccagcagcga cgaagccatg
     2041 aatctcatta aaaacaaaag aaacatgacc ggctacaaga cccttcccta cccgctgaag
     2101 aagcagaacg gcaagatcaa gtacgaatgc aacgtttgcg ccaagacttt cggccagctc
     2161 tccaatctga aggtccacct gagagtgcac agtggagaac ggcctttcaa atgtcagact
     2221 tgcaacaagg gctttactca gctcgcccac ctgcagaaac actacctggt acacacggga
     2281 gaaaagccac atgaatgcca ggtctgccac aagagattta gcagcaccag caatctcaag
     2341 acccacctgc gactccattc tggagagaaa ccataccaat gcaaggtgtg ccctgccaag
     2401 ttcacccagt ttgtgcacct gaaactgcac aagcgtctgc acacccggga gcggccccac
     2461 aagtgctccc agtgccacaa gaactacatc catctctgta gcctcaaggt tcacctgaaa
     2521 gggaactgcg ctgcggcccc ggcgcctggg ctgcccttgg aagatctgac ccgaatcaat
     2581 gaagaaatcg agaagtttga catcagtgac aatgctgacc ggctcgagga cgtggaggat
     2641 gacatcagtg tgatctctgt agtggagaag gaaattctgg ccgtggtcag aaaagagaaa
     2701 gaagaaactg gcctgaaagt gtctttgcaa agaaacatgg ggaatggact cctctcctca
     2761 gggtgcagcc tttatgagtc atcagatcta cccctcatga agttgcctcc cagcaaccca
     2821 ctacctctgg tacctgtaaa ggtcaaacaa gaaacagttg aaccaatgga tccttaatac
     2881 ccagctttct tgtacaaagt ggtataccca tacgatgttc cagattacgc ttagtaatga
     2941 gaaatactca gaagatgtca ctcaccatag tccgcagtga aagataaatg atcagaatct
     3001 tcggaaaggg tgtgttttag agctagaaat agcaagtagg ccactgagca agaactatga
     3061 atgagatctt tagacatatt gcggccgctt tctccgcgat ccagacatga taagatacat
     3121 tgatgagttt ggacaaacca aaactagaat gcagtgaaaa aaatgcctta tttgtgaaat
     3181 ttgtgatgct attgccttat ttgtaaccat tataagctgc aataaacaag ttatctataa
     3241 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt
     3301 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac
     3361 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga
     3421 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa
     3481 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt
     3541 aagaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3601 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3661 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     3721 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     3781 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     3841 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccat gtctccggga
     3901 gctgcatgtg tcagaggttt tcaccgtcat caccgaaaca cgcgagttcg aaagggcctc
     3961 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4021 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4081 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4141 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4201 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4261 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4321 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4381 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4441 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4501 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4561 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4621 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4681 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4741 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4801 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4861 ggttcacgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4921 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4981 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5041 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5101 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5281 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5701 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5761 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5821 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5881 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5941 gcggatgagc gcccaatacg caaaccgcct ctcaccgcgc gttggccgat tcattaatgc
     6001 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aaaccgcctc
     6061 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6121 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6181 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6241 caggaaacag ctatgaccat gattacgcca aggtcgactt aaccctagaa agataatcat
     6301 attgtgacgt acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg
     6361 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
     6421 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac
     6481 aaaaactcaa aatttcttct ataaagtaac aaaactttta gcagtgaaaa aaatgcttta
     6541 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     6601 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     6661 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctggagattc
     6721 atgcgccagg ttttctggtc atgcaccacg ttcgtgggcc ctcaggaacc tccacgtcag
     6781 cggtaactgt gaagcccagt cgctcgtaaa aaggaagatt ccggggagca ctggtttcga
     6841 ggaacgccgg cacgccagcc cgttccgcgg cctcaacacc aggaagaacg acagcgcttc
     6901 ccaaaccttt accttggtga tctgggctga caccgacagt ggcaagaaac cacgcaggct
     6961 cctttggtct atgaggagcc agcaagccct ccatttgctg ttgtgccgcc aacctactcc
     7021 ctgacagctc ggccatccgc ggacctattt ccgcgaacac tgcgccggct tcgacgcttt
     7081 ccggagtagt ccagaccgca actgcggcac catcgtccgc aacccaaact ttaccaatgt
     7141 ccagacccac gcgcgtaaga aacagctctt gcaactcagt taccctctct atgtgtctgt
     7201 ccggatctac ggtatggcgg gtcgctggat aatctgcaaa tgctgcggca agtgttcgga
     7261 cagcccgtgg tacatcgtca cgggtagcga gcctaacagt gggcttatat tccgtcatgg
     7321 ggccggggtt ctcctccacg tcgccggcct gcttcagcag gctgaagttg gtggcgccgc
     7381 tgcccccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc agcatatcaa
     7441 ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg tcaagggcgt
     7501 cggtcggccc gccgctttcg cactttagct gtttctccag gccacatatg attagttcca
     7561 ggccgaaaag gaaggcaggt tcggctccct gccggtcgaa cagctcaatt gcttgtttca
     7621 gaagtggggg catagaatcg gtggtaggtg tctctctttc ctcttttgct acttgatgct
     7681 cctgttcctc caatacgcag cccagtgtaa agtggcccac ggcggacaga gcgtacagtg
     7741 cgttctccag ggagaagcct tgctgacaca ggaacgcgag ctgattttcc agggtttcgt
     7801 actgtttctc tgttgggcgg gtgccgagat gcactttagc cccgtcacga tgtgagagga
     7861 gagcacagcg gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac tcgccttcca
     7921 gggggcagga gtgggtatga tgcctgtcca gcatctcgat tggcagggca tcgagcaggg
     7981 cccgcttgtt cttcacgtgc cagtacaggg taggctgctc aactcccagc ttttgagcga
     8041 gtttccttgt cgtcaggcct tcgataccga ctccattgag taattccaga gcagagttta
     8101 tgactttgct cttgtccagt ctagacatct tatcgtcatc gtctttgtaa tccatggtgg
     8161 cggatcccgc gtcacgacac ctgtgttctg gcggcaaacc cgttgcgaaa aagaacgttc
     8221 acggcgacta ctgcacttat atacggttct cccccaccct cgggaaaaag gcggagccag
     8281 tacacgacat cactttccca gtttaccccg cgccaccttc tctaggcacc ggttcaattg
     8341 ccgacccctc cccccaactt ctcgggcact gtgggccatg tgcgctctgc cccatggtaa
     8401 tagccatgac taatacgtag atgtactgcc aagtaggaaa gtgccataag gtcatgtact
     8461 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     8521 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     8581 gtcaatggaa agtgcctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     8641 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     8701 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca
     8761 ataatcaatg tcataagctc taatcgcata atcgagctaa gagcgattaa tatgcttagc
     8821 tagcttagct aagctagatt tctctattct cccacaatat atagcagcta ttagcttagc
     8881 agtccgaata cgatcgtcca cgatccataa gcgtaacaca tttgagtagc gactgacggg
     8941 caccggagcc actcgagtgg aatta
//
