LOCUS       sequence-439857-       10525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            ZNF541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10525)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 10525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-ZNF541
FEATURES             Location/Qualifiers
     source          1..10525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1944..1953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1954..1978)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2044..2068)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2095..2113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2131..2149
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2167..2185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2203..2221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2239..2257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2275..2293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2311..2329
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     enhancer        2538..2917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2918..3129
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3149..3158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3158..3181
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3182..3925
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             3935..3991
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    4708..4789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4965..5027
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5053..5069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5053..5069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5066..5088)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5077..5093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5101..5107,5108..5125,5126..5131))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5146..5167
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5373..5390)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5544..6132)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5624..5643)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6303..7094,7095..7163))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6926..6945
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7164..7268)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7336..7354
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7393..7415)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7517..7536
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7730..7752
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7744..7761
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7745..7761
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   7772..7806
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    8078..8212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8379..8405)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    8407..8431
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccctttgcg tctcgtctcg tgctgccggg ggaggcctct ctgggaaccc cgggtgtggt
       61 ttttctcctg cgggctgggg agccctctgc ctcggggttt ccagggccgg cgtggagaga
      121 gtccacagca ggagccagtg gtgggggctg ctgcggccac ggcccctgca gcggcctcag
      181 ggcggctggt ttgccctcgg gcgcagggag ctgggaggag actgctgcca cctgggcatg
      241 ggagaagatc cgctgggact tggtgatcat ctggaacacc tgcatctgct cgtggctcac
      301 aagaggttcc tgcgacttga ggaagagctg gcggaagagc ggcgaggcat ccgcagggag
      361 cccgcctgcc ttctgggcct cctggaggcc gggctccccg gggttctggc acaggaagga
      421 gtcgcagtcg accttgacct tcttgggggc gcaggggtca tcttccccgc tggccgacct
      481 ggggtcgctc ggggcctccg cggggaccct gaggagcgcg gcagggaagg gcagagcatc
      541 ctccaggcca ccgccggggc cgggcgggga ctcctccgag gggcttccgc tgctgggtcc
      601 cgggccagac tcggagccct cccgcgaggg cacggccgag ggcttgtgga caacaaacac
      661 gttgttgcct ttgcttttgg gacagcccgg caggttccgg agccactgga agctgtggct
      721 cgatggctgg gaactggcag ggaccgtctg gcctcggaag agaggcaggc aggcaggcac
      781 ggagccgcct tcgggccagc actccgcggt ggaccgggcc tggagcagcg cggtatctgg
      841 ctccggctct ggcgggtcgg gggcgccgtt ctcggcggcc ctggaattag gggctgtgaa
      901 cacgtcagtg gccggctctt tctgtgggag gcaaggctcc tcgggaagct cggtgtccag
      961 cgctgctggg gccgcgtggg ggccggctgg ggtgcaggac gaggaccccg atgaggcggg
     1021 gcagggacag gcagtgttcc tcccttcgct gtctgaagcc cccgccgggg ctgggccagg
     1081 agaaggggtc ttctggtgga cgatgctact cacgatgcgg cgcaggaggt cccggtgggg
     1141 caggagggag ccgggggacc tggcctctgg gggcaccagg gaccgcaggc tgctgggggg
     1201 cggctggccg gccgactcgt gggcgtgggg ggagtccccg caggcctctt cctccggggg
     1261 ggcttccttc aggatgcaca ggccgtgatg gacctcgtag tgccggcgca gagagcggta
     1321 gtcgcagtag ctcttgctgc agccctgctc gatgcacacg aagggctttg tcttctggtg
     1381 ggtgagcatg tgcccggtca ggtggtcctg gcgcttaaag gccttgctgc agattttgca
     1441 gacgtgcttc ctttcctggc tgtgtgtcag gtagtgcttg ctcagagaac tggcgctgct
     1501 gaacaccttc ccgcacaggc tgcagtccag aagtgggttt tgaggggaac tgtgctgccg
     1561 ctttcctttc ctggcactcc ccgaggtggc ccttcctcct tcgtcagcct ctttagcctt
     1621 aagcacacct agccccaggt cttgtaagga tgcctgagac tccgaatctg cgtactcctc
     1681 cagcagcttc acagaatcac tgtccttccc ggagtacagg gacaaggtgt ctaagttgtc
     1741 ctccagcacc tcgctggaca tgtcaggcgt tgggaggctg gggtccgggt ccagaccact
     1801 caggccagca taaagaaagc ctcgcgtgtt gggacccaaa tcccggttga gggtgtcgct
     1861 gcagttgagc ccttggctct ctgaaaatga agggaggtgc atttctgatg ggagggcacc
     1921 ctcgtctcca aggctgtact ggtccatggt ggcagcctgc ttttttgtac aaacttgtgg
     1981 taggctggat cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca
     2041 ggcgatctga cggttcacta aacgagctct gcttatatag gcaagcttat acgttctcta
     2101 tcactgatag ggagtaaact ggatatacgt tctctatcac tgatagggag taaactgtag
     2161 atacgttctc tatcactgat agggagtaaa ctggtcatac gttctctatc actgataggg
     2221 agtaaactcc ttatacgttc tctatcactg atagggagta aagtctgcat acgttctcta
     2281 tcactgatag ggagtaaact cttcatacgt tctctatcac tgatagggag taaactcgag
     2341 gtgataattc cactcgagtg gctccggtgc ccgtcagtcg ctactcaaat gtgttacgct
     2401 tatggatcgt ggacgatcgt attcggactg ctaagctaat agctgctata tattgtggga
     2461 gaatagagaa atctagctta gctaagctag ctaagcatat taatcgctct tagctcgatt
     2521 atgcgattag agcttatgac attgattatt gactagttat taatagtaat caattacggg
     2581 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     2641 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     2701 agtaacgcca ataggcactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     2761 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     2821 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatggcact ttcctacttg
     2881 gcagtacatc tacgtattag tcatggctat taccatgggg cagagcgcac atggcccaca
     2941 gtgcccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     3001 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     3061 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     3121 agaacacagg tgtcgtgacg cgggatccgc caccatggat tacaaagacg atgacgataa
     3181 gatgtctaga ctggacaaga gcaaagtcat aaactctgct ctggaattac tcaatggagt
     3241 cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac
     3301 cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct
     3361 ggacaggcat catacccact cctgccccct ggaaggcgag tcatggcaag actttctgcg
     3421 gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgtgacgggg ctaaagtgca
     3481 tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct
     3541 gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt
     3601 tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac
     3661 acctaccacc gattctatgc ccccacttct gaaacaagca attgagctgt tcgaccggca
     3721 gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca
     3781 gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct
     3841 cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga
     3901 ttttgacctt gacatgctcc ccgggggcag cggcgccacc aacttcagcc tgctgaagca
     3961 ggccggcgac gtggaggaga accccggccc catgacggaa tataagccca ctgttaggct
     4021 cgctacccgt gacgatgtac cacgggctgt ccgaacactt gccgcagcat ttgcagatta
     4081 tccagcgacc cgccataccg tagatccgga cagacacata gagagggtaa ctgagttgca
     4141 agagctgttt cttacgcgcg tgggtctgga cattggtaaa gtttgggttg cggacgatgg
     4201 tgccgcagtt gcggtctgga ctactccgga aagcgtcgaa gccggcgcag tgttcgcgga
     4261 aataggtccg cggatggccg agctgtcagg gagtaggttg gcggcacaac agcaaatgga
     4321 gggcttgctg gctcctcata gaccaaagga gcctgcgtgg tttcttgcca ctgtcggtgt
     4381 cagcccagat caccaaggta aaggtttggg aagcgctgtc gttcttcctg gtgttgaggc
     4441 cgcggaacgg gctggcgtgc cggcgttcct cgaaaccagt gctccccgga atcttccttt
     4501 ttacgagcga ctgggcttca cagttaccgc tgacgtggag gttcctgagg gcccacgaac
     4561 gtggtgcatg accagaaaac ctggcgcatg aatctccaga ggatcataat cagccatacc
     4621 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4681 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4741 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgct aaaagttttg
     4801 ttactttata gaagaaattt tgagtttttg ttttttttta ataaataaat aaacataaat
     4861 aaattgtttg ttgaatttat tattagtatg taagtgtaaa tataataaaa cttaatatct
     4921 attcaaatta ataaataaac ctcgatatac agaccgataa aacacatgcg tcaattttac
     4981 gcatgattat ctttaacgta cgtcacaata tgattatctt tctagggtta agtcgacctt
     5041 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     5101 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5161 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5221 gcattaatga atcggccaac gcgcggggag aggcggtttg cgttgcgctc actgcccgct
     5281 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggtgaga
     5341 ggcggtttgc gtattgggcg ctcatccgct tcctcgctca ctgactcgct gcgctcggtc
     5401 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5461 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5521 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5581 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5641 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5701 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5761 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5821 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5881 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5941 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6001 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6061 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6121 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6181 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6241 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6301 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6361 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6421 cccagtgctg caatgatacc gcgtgaacca cgctcaccgg ctccagattt atcagcaata
     6481 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6541 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6601 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6661 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6721 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6781 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6841 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6901 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6961 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7021 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7081 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7141 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7201 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7261 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     7321 acattaacct ataaaaatag gcgtatcacg aggccctttc gaactcgcgt gtttcggtga
     7381 tgacggtgaa aacctctgac acatgcagct cccggagaca tggtcacagc ttgtctgtaa
     7441 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     7501 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     7561 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     7621 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     7681 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     7741 cgttgtaaaa cgacggccag tgaattctta accctagaaa gatagtctgc gtaaaattga
     7801 cgcatgcatt cttgaaatat tgctctctct ttctaaatag cgcgaatccg tcgctgtgca
     7861 tttaggacat ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca atgcggtaag
     7921 tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta
     7981 cgtgactttt aagatttaac tcatacgata attatattgt tatttcatgt tctacttacg
     8041 tgataactta ttatatatat attttcttgt tatagataac ttgtttattg cagcttataa
     8101 tggttacaaa taaggcaata gcatcacaaa tttcacaaat aaggcatttt tttcactgca
     8161 ttctagtttt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgcggagaa
     8221 agcggccgca atatgtctaa agatctcatt catagttctt gctcagtggc ctacttgcta
     8281 tttctagctc taaaactcaa cgatgggttt atgaaggatc atttatcttt cactgcggac
     8341 tatggtgagt gacatcttct gagtatttct cattactaag cgtaatctgg aacatcgtat
     8401 gggtatacca ctttgtacaa gaaagctggg ttcaccactg cagggggccg atgtcagctc
     8461 ccagctcctc ttcctttagc tggaagggct tcactggcca cttcaccctg atgatgggct
     8521 ccacgtggtc ctgaaggcgg tgccgcttca tatgggcatt tcgactcttg atcttgtcaa
     8581 acaccctttc acactctctg catggaaaaa tgccctggct ctctgcactt cccaacagct
     8641 cgggggtccg cttggagcct gcatttgggc tggagctgag gatggactcc ctcctataac
     8701 tcttggtctt taacttggga gttggatggt ggctgggtct ctcccgaggg ctgcatggga
     8761 ccttttcctc tgtcctttcc acttcctccg gctctctctt gaccctcttt tctagccctg
     8821 gggctcggcc acagtcaaac ttgatcattt ttttccagat gtaataatac tcaacgcact
     8881 gagctaccgt ctttgtctgg atcatcttgt gtatcaagta gaagtccttc ttgtgggcat
     8941 agaacgcctt cttaaaaagc ctcttctcta taggggtcca gacgtctgaa cctgtgtagc
     9001 gatagtcagc gagcaggtgt gtccgtggct tgtggggccc tcggagcaga agagtctcca
     9061 gggcgacctg aacgttgccc tgagcctcgt gcaggcagtg cagagcgagc tccaggttgg
     9121 tgccccctcc tggcatcacg ctggagcatg ccacattgca gagctcggtc actctatcct
     9181 gtgtctctga gctgatcatc atatctcccc atggcttcca gaccagagaa gctacatgct
     9241 cgtcagttcc agccagggat ctctcctgga gttccgggat ctctgcctga aaccggcttc
     9301 ctatattgat atgtggctca atgctgattt tggtgtcatc cttcaccaca cagatgccaa
     9361 aggacccatc cacttgatcg agcatggagc tgatgagctg gtcagggctg gcctgagtgg
     9421 acgtggaaca gagggtgttg aagtacaccc cagagccctc ccggatgggg ctgagcattg
     9481 ggggtggtgt gtagggggag cactggaata agcccttcag gaggcagtcc accaggaaca
     9541 tgggtgaccg caggcggctc tggtggcatc ctgcagggcc aggctcccca gccgtggagg
     9601 gtgggggccg cttcttccgc tttctctgct ggctgctctc cttgctgtct cgctcctccc
     9661 cgtctttctc ttgtcccatg gccatgctcc ctatgtgtcg ggtcactgga accacgggga
     9721 tcgattgggg gaccaccaaa ggggctgcag ctgtggggtc caagggcttc ttggttcctg
     9781 ggggactatg cctgtcccct tctggcctca gcacctgtct tagcggcttg caaaactctg
     9841 tgccaaacac ctgcggttcc tgcttccctc gaggtgacgg ccactggtcg ctgtgaaaac
     9901 acatgtggct gctcagccct ttctccgtat aaaacatctg gctgcagttc ttgcagacaa
     9961 aagtgctcct gggcttcgtc cagtctgtgg gactgccgtt ttgctggtta cctctgcctg
    10021 ccacattctc ttccttcacc ggatggggga gcctgtagat gtttccaccc tggattctgc
    10081 tgaatattgt cagcttggac ttggagggat ctgccggggg cccggccgat gagaaggagg
    10141 ccatggctac ctggctcgga gaagccgcac agcacatatc catcttcgcc ttctctttcc
    10201 ggaagccgga gaatcgcggg gccctggggt tgcccaagag ccggtagcct ccaccccggg
    10261 agcaggctgg gcccttttca ccttttgtga tccttgaagc cgcgccattc tcggctgggg
    10321 cctgcttccc tggcagcgag gctcctggtg gctcctcccc acctaactgg gtctgccgtt
    10381 ggcctgtgga ggggaagatg tcctccaggt ccaaggtccc tttagaggat cgcagctgct
    10441 tggccagaga agagatgtct ggattcctgg aagggtccag agacggtgct gcaaggggcg
    10501 ttggaaccgc ggccactttc agtcc
//
