LOCUS       sequence-439858-       13897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the human receptor LRRC37B HA-tagged in mammalian cells 
            together with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13897)
  AUTHORS   Libe-Philippot B, Lejeune A, Wierda K, Louros N, Erkol E, Vlaeminck 
            I, Beckers S, Gaspariunaite V, Bilheu A, Konstantoulea K, Nyitrai H,
            De Vleeschouwer M, Vennekens KM, Vidal N, Bird TW, Soto DC, Jaspers 
            T, Dewilde M, Dennis MY, Rousseau F, Comoletti D, Schymkowitz J, 
            Theys T, de Wit J, Vanderhaeghen P
  TITLE     LRRC37B is a human modifier of voltage-gated sodium channels and 
            axon excitability in cortical neurons.
  JOURNAL   Cell. 2023 Dec 21;186(26):5766-5783.e25. doi: 
            10.1016/j.cell.2023.11.028.
  PUBMED    38134874
REFERENCE   2  (bases 1 to 13897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11"; journalName: "Cell"; date: "2023-12-21- 
            21"; volume: "186"; issue: "26"; pages: "5766-5783.e25"
COMMENT     Sequence Label: pCIG-LRRC37B-HA
FEATURES             Location/Qualifiers
     source          1..13897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2602..2905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2907..3183
                     /label=chicken beta-actin promoter
     intron          3184..4200
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4124..4146
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4208..4227
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4263..4282
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4263..4281
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4296..4314
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4296..4313
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             7193..7219
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7220..7246
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(7259..7833,7834..7836,7837..7845)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7426..7443)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7653..7672
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7846..7848,7849..7851,7852..8565)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7846..7848,7849..7851,7852..8562)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7891..7912)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8152..8171)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8499..8520
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8589..9177
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8642..8662)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9060..9071)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9180..9196)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9181..9197)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9702..9882
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9908..9925)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9914..10138
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10184..10612
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10271..10290)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10481..10502
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10621..10641)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10626..10955
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10806..10941
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10868..10887
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11003..11050
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11069..11443
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11573..11694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11610..11629)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11664..11683
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11743..11759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11743..11759)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11756..11778)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11767..11783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11791..11797,11798..11815,11816..11821))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11836..11857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11974..11991)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12145..12733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12225..12244)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12904..13695,13696..13764))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13527..13546
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13765..13869)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2641 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2821 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2881 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     2941 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     3001 agcgatgggg gcgggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
     3061 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     3121 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     3181 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     3241 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     3301 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     3361 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
     3421 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
     3481 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     3541 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     3601 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     3661 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     3721 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     3781 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     3841 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     3901 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     3961 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     4021 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     4081 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     4141 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     4201 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgctcgag
     4261 cgtaatacga ctcactatag ggagacccaa gcttgattta ggtgacacta tagaatacaa
     4321 gctacttgtt ctttttgcag gatcggttac catgtcttgg ctgcgtttct ggggcccatg
     4381 gcccctcctt acgtggcaac tattgtcttt actagtcaag gaggctcagc ctctggtgtg
     4441 ggtcaaggac ccgctccagc tgacctctaa ccccctgggg ccacctgagc cctggtcttc
     4501 ccgctcctcc catctcccat gggaatctcc ccatgcacct gctcccccag cagccccggg
     4561 ggactttgat tacctggggc cctctgcttc ttcgcagatg tcagccctgc ctcaggaacc
     4621 aactgaaaat ttggctccat tcctgaagga attggattca gctggagagc tgcccctggg
     4681 gccagagccg ttcttggctg cacatcagga cttaaatgac aagcggactc cagaagaaag
     4741 gctcccagag gtggttccgc ttctcaaccg ggatcagaac caggccctag ttcagcttcc
     4801 tcgcctcaag tgggttcaaa ctacagatct agatcgggct gcaggtcatc aggcagatga
     4861 aatacttgtt ccactagaca gtaaggtttc aagaccaacc aaatttgttg tttcgcccaa
     4921 gaacctgaag aaagatctag ctgaacgttg gagccttcct gagattgttg ggattccaca
     4981 ccaattatcc aaacctcagc gtcagaaaca gactttgcca gatgattatt tgagtatgga
     5041 cacactgtat cccggcagcc tacctccaga actccgggtg aacgcagatg agcctccagg
     5101 gcctcctgag caagttggac tttctcaatt ccatctagag cccaaaagtc aaaatccaga
     5161 gacccttgaa gacatccagt cctcttcact ccaggaagaa gccccagcgc agcttctaca
     5221 gctccctcag gaggtagaac cttcaaccca gcaggaggcc ccagctctgc ctccagagtc
     5281 ctctatggag agtctagctc aaactccact gaatcatgaa gtgacagttc aacctccagg
     5341 tgaggatcaa gctcattata atttgcccaa gtttacagtc aaacctgcag atgtggaggt
     5401 taccatgact tcagagccta aaaatgagac agaatctacc caagcccagc aggaggcccc
     5461 aattcagcct cccgaggagg cggaaccttc ttctacagcc ctgaggacta cagatcctcc
     5521 tccagaacac cctgaggtga cacttccacc ttcagacaag ggtcaggctc agcattcaca
     5581 cctgactgaa gccacagttc aacctctgga cctggagctt agcataacta cagagcctac
     5641 tacagaggtt aaaccgtctc caaccacgga ggaaacctca gctcagcctc cagacccggg
     5701 gcttgccata actccagaac ccactacaga gattggacat tccacagccc tggagaagac
     5761 tagagctcct catccagacc aggttcagac tctgcatcga agcctgactg aagtcacagg
     5821 tccacctaca aagttagaat cttcgcagga ttcattggtg cagtctgaaa ctgcaccaga
     5881 ggaacagaag gcctccacaa gcaccaacat atgtgagctc tgcacctgcg gagatgagac
     5941 tctgtcatgt gttggtctca gcccaaagca gaggctccgc caagtgcctg tgccagagcc
     6001 cgacacctac aatggcatct tcaccacctt aaatttccaa ggaaactata tttcatacct
     6061 tgatggaaat gtatggaaag catacagttg gaccgagaaa ctaattctca gtgaaaatta
     6121 tttgactgaa ttacctaagg attcatttga aggcctgcta tacctccagt atttagattt
     6181 atcctgcaat aaaatacgat atattgaaag acaaacattt gaatcactac catttttgca
     6241 gtatataaat ctgggctgca atttaattac aaaactgagc cttggaacat ttcaggcctg
     6301 gcacggaatg cagtttttac acaacttaat tctcaatcgc aatcctctga ctactgtcga
     6361 agatccatat ctctttgaac tgccggcatt aaaatatcta gacatgggaa caacacacat
     6421 cacacttaca acacttaaga acattctcac gatgactgtt gaactggaaa aactgatctt
     6481 acctagccat atggcctgct gcctctgcca atttaaaaat agcattgagg ctgtctgcaa
     6541 gacagtcaag ctgcattgca acactgcatg tctgactaac agcatacatt gtcctgaaga
     6601 agcatctgta gggaatccag aaggagcgtt catgaagatg ttacaagccc ggaagcagca
     6661 catgagcact cagctgacta ttgagtcgga ggcgccctca gacagcagtg gcatcaactt
     6721 gtcaggcttt gggggtgatc agcttgaaat tcagctaacc gagcagctac ggtccctcat
     6781 ccccaacgag gatgtgagaa agttcatgtc tcatgttatc cggaccttga aaatggaatg
     6841 ttcagaaaca catgtgcaag ggagctgtgc caagctcatg ttgcgaacag gcctcctgat
     6901 gaagcttctc agcgagcagc aggaagcaaa ggcattgaat gtagaatggg atacggacca
     6961 acaaaaaaca aattatatta atgagaacat ggaacagaat gaacagaaag agcagaagtc
     7021 aagtgagctc atgaaagaag ttccaggaga tgactataag aacaaactca tcttcgcaat
     7081 atctgtgact gtaatactaa taattttgat tataattttt tgtcttatag aggtgaattc
     7141 acataaaagg gcatcagaaa aatacaaaga caacccatca atatcaggag cctacccata
     7201 cgatgttcca gattacgctt acccatacga tgttccagat tacgcttaga acgatccgcc
     7261 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     7321 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     7381 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     7441 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     7501 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     7561 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     7621 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     7681 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca
     7741 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     7801 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc
     7861 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     7921 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     7981 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     8041 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     8101 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     8161 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     8221 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     8281 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     8341 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     8401 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     8461 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     8521 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaaattc gatatcaagc
     8581 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     8641 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     8701 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     8761 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     8821 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     8881 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     8941 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     9001 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     9061 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     9121 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     9181 gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     9241 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     9301 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     9361 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     9421 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     9481 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     9541 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     9601 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     9661 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     9721 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9781 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     9841 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     9901 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     9961 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
    10021 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
    10081 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
    10141 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
    10201 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    10261 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    10321 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    10381 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    10441 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    10501 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    10561 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
    10621 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
    10681 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
    10741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
    10801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    10861 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
    10921 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
    10981 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
    11041 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
    11101 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
    11161 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
    11221 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
    11281 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
    11341 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
    11401 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt
    11461 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
    11521 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
    11581 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    11641 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    11701 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
    11761 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    11821 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    11881 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
    11941 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    12001 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    12061 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    12121 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    12181 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    12241 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    12301 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    12361 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    12421 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    12481 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    12541 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    12601 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    12661 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    12721 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    12781 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    12841 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    12901 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    12961 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    13021 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    13081 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    13141 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    13201 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    13261 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    13321 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    13381 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    13441 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    13501 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    13561 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    13621 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    13681 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    13741 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    13801 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    13861 ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
