LOCUS       sequence-439864-       15181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the human receptor LRRC37B (cre-dependant) in mammalian 
            cells together with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15181)
  AUTHORS   Libe-Philippot B, Lejeune A, Wierda K, Louros N, Erkol E, Vlaeminck 
            I, Beckers S, Gaspariunaite V, Bilheu A, Konstantoulea K, Nyitrai H,
            De Vleeschouwer M, Vennekens KM, Vidal N, Bird TW, Soto DC, Jaspers 
            T, Dewilde M, Dennis MY, Rousseau F, Comoletti D, Schymkowitz J, 
            Theys T, de Wit J, Vanderhaeghen P
  TITLE     LRRC37B is a human modifier of voltage-gated sodium channels and 
            axon excitability in cortical neurons.
  JOURNAL   Cell. 2023 Dec 21;186(26):5766-5783.e25. doi: 
            10.1016/j.cell.2023.11.028.
  PUBMED    38134874
REFERENCE   2  (bases 1 to 15181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11"; journalName: "Cell"; date: "2023-12-21- 
            21"; volume: "186"; issue: "26"; pages: "5766-5783.e25"
COMMENT     Sequence Label: pCIG-LSL-LRRC37B
FEATURES             Location/Qualifiers
     source          1..15181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2602..2905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2907..3182
                     /label=chicken beta-actin promoter
     intron          3183..4199
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4123..4145
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4207..4226
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4262..4281
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4262..4280
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4295..4313
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4295..4312
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(4351..4384)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     intron          4918..4983
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5113..5133
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5558..5692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5734..5767)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(8662..9236,9237..9239,9240..9248)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8829..8846)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9056..9075
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9249..9251,9252..9254,9255..9968)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9249..9251,9252..9254,9255..9965)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9294..9315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9555..9574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9902..9923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    9992..10580
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10045..10065)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10463..10474)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10583..10599)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             10986..11166
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11192..11209)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11198..11422
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11468..11896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11555..11574)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11765..11786
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11905..11925)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11910..12239
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12090..12225
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12152..12171
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12287..12334
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12353..12727
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12857..12978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13027..13043)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13027..13043)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13040..13062)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13051..13067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13075..13081,13082..13099,13100..13105))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13120..13141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13258..13275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13429..14017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13509..13528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14188..14979,14980..15048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14811..14830
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15049..15153)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcc gcgttacata acttacggta aatggcccgc ctggctgacc
     2641 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     2701 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     2761 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2821 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2881 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
     2941 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
     3001 agcgatgggg gcgggggggg ggggggggcg cgccaggcgg ggcggggcgg ggcgaggggc
     3061 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     3121 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     3181 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     3241 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     3301 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     3361 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
     3421 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
     3481 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     3541 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     3601 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     3661 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     3721 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     3781 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     3841 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     3901 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     3961 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     4021 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     4081 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     4141 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     4201 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttgctcgagc
     4261 gtaatacgac tcactatagg gagacccaag cttgatttag gtgacactat agaatacaag
     4321 ctacttgttc tttttgcagg atcggctagt ataacttcgt atagcataca ttatacgaag
     4381 ttatattaag ggttccggat cctcggggac accaaatatg gcgatctcgg ccttttcgtt
     4441 tcttggagct gggacatgtt tgccatcgat ccatctacca ccagaacggc cgttagatct
     4501 gctgccaccg ttgtttccac cgaagaaacc accgttgccg taaccaccac gacggttgtt
     4561 gctaaagaag ctgccaccgc cacggccacc gttgtagccg ccgttgttgt tattgtagtt
     4621 gctactgtta tttctggcac ttcttggttt tcctcttaag tgaggaggaa cataaccatt
     4681 ctcgttgttg tcgttgatgc ttaaattttg cacttgttcg ctcagttcag ccataatatg
     4741 aaatgctttt cttgttgttc ttacggaata ccacttgcca cctatcacca caactaactt
     4801 tttcccgttc ctccatctct tttatatttt ttttctcgag ggatctttgt gaaggaacct
     4861 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     4921 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     4981 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     5041 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     5101 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     5161 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     5221 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     5281 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     5341 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     5401 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
     5461 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     5521 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     5581 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5641 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgacatgg
     5701 taagtaagct tgggctgcag gtcgagggac ctaataactt cgtatagcat acattatacg
     5761 aagttatgct agcggctact tgttcttttt gcaggatcgg ttaccaccat gtcttggctg
     5821 cgtttctggg gcccatggcc cctccttacg tggcaactat tgtctttact agtcaaggag
     5881 gctcagcctc tggtgtgggt caaggacccg ctccagctga cctctaaccc cctggggcca
     5941 cctgagccct ggtcttcccg ctcctcccat ctcccatggg aatctcccca tgcacctgct
     6001 cccccagcag ccccggggga ctttgattac ctggggccct ctgcttcttc gcagatgtca
     6061 gccctgcctc aggaaccaac tgaaaatttg gctccattcc tgaaggaatt ggattcagct
     6121 ggagagctgc ccctggggcc agagccgttc ttggctgcac atcaggactt aaatgacaag
     6181 cggactccag aagaaaggct cccagaggtg gttccgcttc tcaaccggga tcagaaccag
     6241 gccctagttc agcttcctcg cctcaagtgg gttcaaacta cagatctaga tcgggctgca
     6301 ggtcatcagg cagatgaaat acttgttcca ctagacagta aggtttcaag accaaccaaa
     6361 tttgttgttt cgcccaagaa cctgaagaaa gatctagctg aacgttggag ccttcctgag
     6421 attgttggga ttccacacca attatccaaa cctcagcgtc agaaacagac tttgccagat
     6481 gattatttga gtatggacac actgtatccc ggcagcctac ctccagaact ccgggtgaac
     6541 gcagatgagc ctccagggcc tcctgagcaa gttggacttt ctcaattcca tctagagccc
     6601 aaaagtcaaa atccagagac ccttgaagac atccagtcct cttcactcca ggaagaagcc
     6661 ccagcgcagc ttctacagct ccctcaggag gtagaacctt caacccagca ggaggcccca
     6721 gctctgcctc cagagtcctc tatggagagt ctagctcaaa ctccactgaa tcatgaagtg
     6781 acagttcaac ctccaggtga ggatcaagct cattataatt tgcccaagtt tacagtcaaa
     6841 cctgcagatg tggaggttac catgacttca gagcctaaaa atgagacaga atctacccaa
     6901 gcccagcagg aggccccaat tcagcctccc gaggaggcgg aaccttcttc tacagccctg
     6961 aggactacag atcctcctcc agaacaccct gaggtgacac ttccaccttc agacaagggt
     7021 caggctcagc attcacacct gactgaagcc acagttcaac ctctggacct ggagcttagc
     7081 ataactacag agcctactac agaggttaaa ccgtctccaa ccacggagga aacctcagct
     7141 cagcctccag acccggggct tgccataact ccagaaccca ctacagagat tggacattcc
     7201 acagccctgg agaagactag agctcctcat ccagaccagg ttcagactct gcatcgaagc
     7261 ctgactgaag tcacaggtcc acctacaaag ttagaatctt cgcaggattc attggtgcag
     7321 tctgaaactg caccagagga acagaaggcc tccacaagca ccaacatatg tgagctctgc
     7381 acctgcggag atgagactct gtcatgtgtt ggtctcagcc caaagcagag gctccgccaa
     7441 gtgcctgtgc cagagcccga cacctacaat ggcatcttca ccaccttaaa tttccaagga
     7501 aactatattt cataccttga tggaaatgta tggaaagcat acagttggac cgagaaacta
     7561 attctcagtg aaaattattt gactgaatta cctaaggatt catttgaagg cctgctatac
     7621 ctccagtatt tagatttatc ctgcaataaa atacgatata ttgaaagaca aacatttgaa
     7681 tcactaccat ttttgcagta tataaatctg ggctgcaatt taattacaaa actgagcctt
     7741 ggaacatttc aggcctggca cggaatgcag tttttacaca acttaattct caatcgcaat
     7801 cctctgacta ctgtcgaaga tccatatctc tttgaactgc cggcattaaa atatctagac
     7861 atgggaacaa cacacatcac acttacaaca cttaagaaca ttctcacgat gactgttgaa
     7921 ctggaaaaac tgatcttacc tagccatatg gcctgctgcc tctgccaatt taaaaatagc
     7981 attgaggctg tctgcaagac agtcaagctg cattgcaaca ctgcatgtct gactaacagc
     8041 atacattgtc ctgaagaagc atctgtaggg aatccagaag gagcgttcat gaagatgtta
     8101 caagcccgga agcagcacat gagcactcag ctgactattg agtcggaggc gccctcagac
     8161 agcagtggca tcaacttgtc aggctttggg ggtgatcagc ttgaaattca gctaaccgag
     8221 cagctacggt ccctcatccc caacgaggat gtgagaaagt tcatgtctca tgttatccgg
     8281 accttgaaaa tggaatgttc agaaacacat gtgcaaggga gctgtgccaa gctcatgttg
     8341 cgaacaggcc tcctgatgaa gcttctcagc gagcagcagg aagcaaaggc attgaatgta
     8401 gaatgggata cggaccaaca aaaaacaaat tatattaatg agaacatgga acagaatgaa
     8461 cagaaagagc agaagtcaag tgagctcatg aaagaagttc caggagatga ctataagaac
     8521 aaactcatct tcgcaatatc tgtgactgta atactaataa ttttgattat aattttttgt
     8581 cttatagagg tgaattcaca taaaagggca tcagaaaaat acaaagacaa cccatcaata
     8641 tcaggagcct agaacgatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     8701 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     8761 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     8821 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     8881 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     8941 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     9001 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     9061 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     9121 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     9181 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     9241 ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     9301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     9361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     9421 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     9481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     9541 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     9601 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     9661 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     9721 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     9781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     9841 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     9901 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     9961 acaagtaaaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
    10021 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
    10081 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
    10141 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
    10201 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
    10261 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
    10321 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
    10381 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
    10441 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
    10501 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
    10561 cctttgggcc gcctccccgc atcgataccg tcgacctcgc tttaagacca atgacttaca
    10621 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
    10681 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc
    10741 ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt
    10801 gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca
    10861 cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag
    10921 agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact
    10981 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    11041 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    11101 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    11161 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
    11221 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
    11281 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
    11341 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
    11401 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
    11461 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    11521 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    11581 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    11641 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    11701 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    11761 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    11821 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    11881 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
    11941 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
    12001 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    12061 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
    12121 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
    12181 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
    12241 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
    12301 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
    12361 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
    12421 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
    12481 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
    12541 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
    12601 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
    12661 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
    12721 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
    12781 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
    12841 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    12901 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    12961 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
    13021 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    13081 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    13141 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    13201 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    13261 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    13321 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    13381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    13441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    13501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    13561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    13621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    13681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    13741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    13801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    13861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    13921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    13981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    14041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    14101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    14161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    14221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    14281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    14341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    14401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    14461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    14521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    14581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    14641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    14701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    14761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    14821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    14881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    14941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    15001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    15061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    15121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    15181 c
//
