LOCUS       sequence-439865-       10777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            YTHDC2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 10777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-YTHDC2
FEATURES             Location/Qualifiers
     source          1..10777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4692..4716)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4718..4744
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4911..5045
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5317..5351
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5362..5379)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5362..5378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5371..5393)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5587..5606)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5708..5730
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5769..5787)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5855..5959
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5960..6028,6029..6820)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6178..6197)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6991..7579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7480..7499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7733..7750
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7956..7977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7992..7997,7998..8015,8016..8022)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8030..8046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8035..8057
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8054..8070
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8054..8070
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   8096..8158
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    8334..8415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9132..9188)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(9198..9941)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(9942..9965)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9965..9974
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9994..10205)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        10206..10585
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagcagg ctgccaccat gagccgacca tcttccgtat
      421 ctcccagaca acctgcaccc ggcggaggag gcgggggggg accatcacct tgcgggccag
      481 gtggaggagg gcgagctaaa ggtcttaaag atatccgaat cgacgaagaa gtaaagattg
      541 ctgtaaacat agcacttgaa cgttttcgct atggcgatca acgtgagatg gagttcccca
      601 gctccctgac atccacggag cgtgcgttca tacaccggtt atcccaaagc ctgggactgg
      661 tgagcaagtc caaggggaaa ggggcgaacc gttatctgac agtcaagaag aaggacggca
      721 gcgagaccgc ccacgctatg atgacttgca acctgacaca caacaccaag cacgccgtac
      781 gctctctgat ccagcggttc cccgtgacta acaaggaaag gactgagtta ttaccaaaga
      841 ccgagcgagg caacgttttc gccgtcgagg cggagaatag ggagatgtct aaaacgtccg
      901 gacgccttaa taacggtatc ccacaaatcc ctgtcaagag gggtgagtct gagttcgaca
      961 gcttccggca atccctgccc gttttcgaaa agcaagagga gatcgtgaag attatcaaag
     1021 agaacaaggt cgtcctgatc gtcggtgaga ccggttccgg gaaaactacc caaatcccac
     1081 aatttctgct ggacgactgt ttcaagaacg gcattccttg tagaattttc tgcacacagc
     1141 ctcggcgcct cgccgcgatt gcagttgcag agcgcgtagc tgctgaacga agagagagaa
     1201 tcgggcagac catagggtac caaatacggc tcgagagtcg ggtgtcaccc aaaactctgc
     1261 tcaccttctg cacaaacgga gttctgctcc gcactcttat ggctggggac tctaccctct
     1321 ctacagtcac tcacgtcata gttgacgagg ttcacgagag agacaggttc tctgacttcc
     1381 tgttaacgaa actccgcgac ctgctccaga aacatcctac cctgaagctg atcctgtcat
     1441 ctgccgcgct ggacgtgaac ctgttcattc gctacttcgg tagctgccct gtaatttaca
     1501 ttcaaggtcg acccttcgag gttaaggaaa tgttcctcga ggacatcctt cgcaccacag
     1561 gttacacgaa taaggaaatg ctgaagtaca agaaagagaa gcaacaagag gaaaagcagc
     1621 agactacctt gactgagtgg tatagcgccc aggagaactc ttttaaaccg gagagccaaa
     1681 gacaacgcac agtgttaaac gtcaccgacg aatacgatct cttagacgac ggcggcgacg
     1741 ccgttttctc tcaattgaca gagaaggacg tcaactgtct ggagccctgg ctgatcaaag
     1801 agatggacgc ctgtctgagc gacatttggc tgcacaagga catagacgct ttcgcccaag
     1861 tgttccacct gatcctgacc gagaacgtgt ccgtggacta taggcactcc gagacatctg
     1921 ccactgcgct tatggtggcg gccggcaggg gcttcgcctc tcaggtcgag caactgattt
     1981 ctatgggcgc aaacgtgcac agcaaggcct ctaacgggtg gatggccctg gactgggcca
     2041 agcatttcgg acaaaccgag atcgttgacc tgctggagtc atattctgcg accctcgagt
     2101 tcggcaacct cgacgagtcc tcattggtgc agaccaacgg gtctgatctg agcgcagagg
     2161 atcgagaact gctcaaggca taccaccact cctttgacga cgagaaggtt gatctggacc
     2221 ttattatgca cctgctgtat aacatatgtc actcctgcga cgccggcgcc gtgctgatct
     2281 tcttgccagg ttacgatgag atcgtgggtt tgagggaccg gattctcttc gacgataaac
     2341 gcttcgcaga ttccacccac cgttatcagg tgttcatgct gcactccaac atgcagacca
     2401 gcgaccagaa gaaggtgctg aagaatccgc ccgccggcgt acgcaagatt atcctcagca
     2461 caaacatcgc ggagacgtct attactgtga acgacgtggt gttcgtgatc gactccggca
     2521 aagttaagga aaagtctttc gacgcactca acttcgtgac catgttgaag atggtgtgga
     2581 tcagcaaggc atcagcaatt caaaggaagg gaagagcggg cagatgccgg cccgggatct
     2641 gcttccgact cttttcccgc ctgagatttc aaaacatgct cgagttccaa acaccagagc
     2701 tgctgcgaat gcctctgcaa gagctgtgtc ttcacactaa acttctcgcg cccgtgaact
     2761 gccctatagc cgacttcttg atgaaggccc cagagccgcc ccctgcactg atagtgcgga
     2821 acgccgtgca gatgctgaaa actatcgacg ctatggacac ttgggaagac ctcacggagt
     2881 tgggttacca cctggccgat cttcccgtgg agcctcacct gggcaagatg gtactgtgcg
     2941 ccgtcgtcct taaatgcttg gatcctattc tcaccatcgc ctgtaccctt gcatacagag
     3001 accccttcgt gctcccgaca caagctagcc agaagcgcgc cgcaatgctc tgccgcaaga
     3061 gattcaccgc cggcgccttt tccgatcaca tggcactcct gagggccttt caagcgtggc
     3121 agaaggctag gagcgacgga tgggaaagag ctttctgcga gaagaacttc ctgagtcaag
     3181 caacgatgga gattataatt ggtatgcgga cgcaactgct gggacagctg cgggcgagtg
     3241 gattcgtcag ggccaggggc ggcggggata taagagatgt gaatacgaat tccgaaaact
     3301 gggcggtggt gaaggcagct ctggtcgccg ggatgtaccc caacctcgtt catgttgatc
     3361 gcgaaaactt ggtcctgacg ggtcccaaag aaaagaaggt caggttccac cccgcctctg
     3421 tgctgagcca accccagtac aagaaaatcc cacccgcaaa cgggcaggcc gccgccatca
     3481 aagctttgcc gaccgactgg ctgatctacg acgagatgac tagggcacac cgcatcgcaa
     3541 acatccgctg ctgctctgct gttaccccgg tgaccattct ggtcttttgc gggcctgccc
     3601 ggctggcctc taacgccctg caagagccca gcagcttccg cgttgacggt atccctaacg
     3661 attcttctga cagcgagatg gaagataaga ccaccgcgaa cttagctgcg ctcaagttgg
     3721 acgaatggtt acactttact ctcgaacctg aagcggcctc tttgcttctc caactgaggc
     3781 agaaatggca ctctttgttc ttgcggcgca tgcgggcccc ttcaaagccc tggagccagg
     3841 tggacgaggc aactattcgt gccatcattg ccgtgctgtc tacagaggag caatccgccg
     3901 ggctccagca gccatccgga ataggacagc ggccgcgacc catgagcagc gaggaattgc
     3961 cgctggcttc ctcatggcgg tccaacaact ctcgcaagtc tagtgccgac accgagttca
     4021 gcgacgaatg cacgaccgcc gagagggtcc ttatgaagtc cccctcaccc gccctgcatc
     4081 ccccgcagaa atataaggac aggggcatac tccaccccaa gcgcgggacc gaagataggt
     4141 ctgaccaatc ttccctcaag agcactgata gttcctctta tccgtcaccg tgcgcatccc
     4201 ccagcccgcc ctcttctggc aaaggaagca agagcccaag tcccaggccc aatatgccag
     4261 tgagatattt tatcatgaaa tcttccaact taaggaatct cgagatcagc cagcaaaagg
     4321 gaatttggag caccacccca tccaacgaga ggaaattgaa cagagcattc tgggagtctt
     4381 caatcgtgta tctggtcttc agtgtgcagg gcagtgggca ctttcaaggg ttctcccgca
     4441 tgtccagtga aatcgggaga gagaaatccc aagattgggg ttcagccggg ttgggcggtg
     4501 tgttcaaagt tgaatggatc cgtaaagagt cattgccttt ccagttcgct catcacctgc
     4561 tgaacccctg gaacgataat aagaaggtcc aaatttcacg cgacggccaa gagttagagc
     4621 cattggtcgg ggagcaactt ttgcaactgt gggagcggct cccgctgggc gagaagaata
     4681 ccaccgacta gacccagctt tcttgtacaa agtggtatac ccatacgatg ttccagatta
     4741 cgcttagtaa tgagaaatac tcagaagatg tcactcacca tagtccgcag tgaaagataa
     4801 atgatctgcc tattataggg gtgccggttt tagagctaga aatagcaagt aggccactga
     4861 gcaagaacta tgaatgagat ctttagacat attgcggccg ctttctccgc gatccagaca
     4921 tgataagata cattgatgag tttggacaaa ccaaaactag aatgcagtga aaaaaatgcc
     4981 ttatttgtga aatttgtgat gctattgcct tatttgtaac cattataagc tgcaataaac
     5041 aagttatcta taacaagaaa atatatatat aataagttat cacgtaagta gaacatgaaa
     5101 taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat aatcatgcgt
     5161 cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc attgacaagc
     5221 acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc gacggattcg
     5281 cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt acgcagacta
     5341 tctttctagg gttaagaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5401 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5461 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     5521 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     5581 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5641 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5701 catgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acacgcgagt
     5761 tcgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5821 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5881 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5941 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6001 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6061 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6121 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6181 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6241 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6301 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6361 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6421 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6481 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6541 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6601 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6661 agccggtgag cgtggttcac gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6721 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6781 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6841 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6901 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6961 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7021 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7081 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7141 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7201 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7261 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7321 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7381 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7441 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7501 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7561 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7621 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7681 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7741 agtgagcgag gaagcggatg agcgcccaat acgcaaaccg cctctcaccg cgcgttggcc
     7801 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7861 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     7921 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     7981 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8041 taacaatttc acacaggaaa cagctatgac catgattacg ccaaggtcga cttaacccta
     8101 gaaagataat catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg
     8161 ttttatcggt ctgtatatcg aggtttattt attaatttga atagatatta agttttatta
     8221 tatttacact tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt
     8281 attaaaaaaa aacaaaaact caaaatttct tctataaagt aacaaaactt ttagcagtga
     8341 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     8401 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     8461 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     8521 cctctggaga ttcatgcgcc aggttttctg gtcatgcacc acgttcgtgg gccctcagga
     8581 acctccacgt cagcggtaac tgtgaagccc agtcgctcgt aaaaaggaag attccgggga
     8641 gcactggttt cgaggaacgc cggcacgcca gcccgttccg cggcctcaac accaggaaga
     8701 acgacagcgc ttcccaaacc tttaccttgg tgatctgggc tgacaccgac agtggcaaga
     8761 aaccacgcag gctcctttgg tctatgagga gccagcaagc cctccatttg ctgttgtgcc
     8821 gccaacctac tccctgacag ctcggccatc cgcggaccta tttccgcgaa cactgcgccg
     8881 gcttcgacgc tttccggagt agtccagacc gcaactgcgg caccatcgtc cgcaacccaa
     8941 actttaccaa tgtccagacc cacgcgcgta agaaacagct cttgcaactc agttaccctc
     9001 tctatgtgtc tgtccggatc tacggtatgg cgggtcgctg gataatctgc aaatgctgcg
     9061 gcaagtgttc ggacagcccg tggtacatcg tcacgggtag cgagcctaac agtgggctta
     9121 tattccgtca tggggccggg gttctcctcc acgtcgccgg cctgcttcag caggctgaag
     9181 ttggtggcgc cgctgccccc ggggagcatg tcaaggtcaa aatcgtcaag agcgtcagca
     9241 ggcagcatat caaggtcaaa gtcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa
     9301 tcgtcaaggg cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc caggccacat
     9361 atgattagtt ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca
     9421 attgcttgtt tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt
     9481 gctacttgat gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac
     9541 agagcgtaca gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt
     9601 tccagggttt cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtca
     9661 cgatgtgaga ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa gtcttgccat
     9721 gactcgcctt ccagggggca ggagtgggta tgatgcctgt ccagcatctc gattggcagg
     9781 gcatcgagca gggcccgctt gttcttcacg tgccagtaca gggtaggctg ctcaactccc
     9841 agcttttgag cgagtttcct tgtcgtcagg ccttcgatac cgactccatt gagtaattcc
     9901 agagcagagt ttatgacttt gctcttgtcc agtctagaca tcttatcgtc atcgtctttg
     9961 taatccatgg tggcggatcc cgcgtcacga cacctgtgtt ctggcggcaa acccgttgcg
    10021 aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac cctcgggaaa
    10081 aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc ttctctaggc
    10141 accggttcaa ttgccgaccc ctccccccaa cttctcgggc actgtgggcc atgtgcgctc
    10201 tgccccatgg taatagccat gactaatacg tagatgtact gccaagtagg aaagtgccat
    10261 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
    10321 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
    10381 tccacccatt gacgtcaatg gaaagtgcct attggcgtta ctatgggaac atacgtcatt
    10441 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
    10501 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
    10561 taataactag tcaataatca atgtcataag ctctaatcgc ataatcgagc taagagcgat
    10621 taatatgctt agctagctta gctaagctag atttctctat tctcccacaa tatatagcag
    10681 ctattagctt agcagtccga atacgatcgt ccacgatcca taagcgtaac acatttgagt
    10741 agcgactgac gggcaccgga gccactcgag tggaatt
//
