LOCUS       sequence-439867-        9008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homology directed repair template for knocking in mGreenLantern 
            reporter to REC8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9008)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 9008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pHDR_REC8-T2A-mGreenLantern-puroTK
FEATURES             Location/Qualifiers
     source          1..9008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1785..1804
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1785..1803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2834..2887
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2888..2890,2891..2893,2894..3607)
                     /codon_start=1
                     /product="monomeric GFP derivative that displays 
                     exceptionally high brightness in cells (Campbell et al., 
                     2020)"
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(2933..2954)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    3608..3639
                     /label=rox
                     /bound_moiety="Dre recombinase"
     promoter        3689..4188
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3724..3747)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4121..4140
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4202..4211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4208..4807
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4707..4727
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4814..5809
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(5831..5848)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5837..6061
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6109..6140
                     /label=rox
                     /bound_moiety="Dre recombinase"
     CDS             7423..7995
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          8072..8137
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     rep_origin      complement(8420..9008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8500..8519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtaccggg ccccccctcg agtggagccc agtgttcccc ttatggtgtc
     1861 tttaggtaag cacctagaga agaggcgcag tgggaccaca ccctaaccac tgtcccagga
     1921 aactagtatc ccaactgctg aagctgtttt atgagtgaag gcgtgtgtgt gtgacataag
     1981 gaagcacgga ctggtcctcc tcagctctcc cacctatgtg cttttaacaa gtcaccgcac
     2041 ggctctgcca tctacttaca ccaggtggcc acaaggccct aactgccacc caagcagaca
     2101 cccactagcg cctttcctcc cccaacagag atctccctag aggcagctga agaggagaag
     2161 tcccgcatca gcctcatccc accagaagaa cggtggtaag cggccaggct ccgtgggagc
     2221 cagggcccac agcccttggc caggtggtgg aaacagctgc tgggatgggt atgccccttg
     2281 tcactgtcac agctgccacc ttccctactc tctcatgtcc tcctagggcc tggcctgagg
     2341 tggaggcgcc agaagctcct gcattgcccg tggtgcctga actccctgag gtgcccatgg
     2401 agatgccttt ggtgctgccc ccagagctcg agctgctctc actggaagca gtgcacaggt
     2461 accagggagg tggcaccttg atggggtgga cccgggctga agcctctgct aatggttctt
     2521 gatccctata gggcagtggc actggagctg caggctaaca gggagcccga cttcagcagc
     2581 ctggtgtcac ctctcagccc ccgcaggatg gctgcccggg tcttctacct gctcctgggt
     2641 gagtgtatgc atgtgtgtgt gtgtatgtgg ggcagggaca cagagaccag aggcccgtac
     2701 agggactccc ccgacctgcc ctctcctcgc ctcttgacca gtgctctcag cgcaacagat
     2761 tcttcacgtg aaacaagaaa agccatatgg tcgcctcctg atccagccgg ggcccagatt
     2821 ccacgggtct ggcgagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
     2881 tggcccaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2941 gctggacggc gacgtaaacg gccacaagtt cagcgtccgc ggcgagggcg agggcgatgc
     3001 caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3061 gcccaccctc gtgaccacct taggctacgg cgtggcctgc ttcgcccgct accccgacca
     3121 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3181 catctctttc aaggacgacg gtacctacaa gacccgcgcc gaggtgaagt tcgagggcga
     3241 caccctggtg aaccgcatcg tgctgaaggg catcgacttc aaggaggacg gcaacatcct
     3301 ggggcacaag ctggagtaca acttcaacag ccacaaggtc tatatcacgg ccgacaagca
     3361 gaagaacggc atcaaggcta acttcaagac ccgccacaac gttgaggacg gcggcgtgca
     3421 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3481 caaccactac ctgagccatc agtccaaact gagcaaagac cccaacgaga agcgcgatca
     3541 catggtcctg aaggagaggg tgaccgccgc cgggattaca catgacatgg acgagctgta
     3601 caagtaataa ctttaaataa tgccaattat ttaaagttac atatgagaaa gtataggaac
     3661 ttcagcttga tatcgaatta attctaccgg gtaggggagg cgcttttccc aaggcagtct
     3721 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     3781 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     3841 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     3901 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     3961 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     4021 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     4081 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     4141 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccaa
     4201 cgccaccatg gggaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4261 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4321 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4381 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4441 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4501 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4561 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4621 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4681 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4741 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     4801 cggtgccgga tccatgccca cgctactgcg ggtttatata gacggtcctc acgggatggg
     4861 gaaaaccacc accacgcaac tgctggtggc cctgggttcg cgcgacgata tcgtctacgt
     4921 acccgagccg atgacttact ggcaggtgct gggggcttcc gagacaatcg cgaacatcta
     4981 caccacacaa caccgcctcg accagggtga gatatcggcc ggggacgcgg cggtggtaat
     5041 gacaagcgcc cagataacaa tgggcatgcc ttatgccgtg accgacgccg ttctggctcc
     5101 tcatatcggg ggggaggctg ggagctcaca tgccccgccc ccggccctca ccctcatctt
     5161 cgaccgccat cccatcgccg ccctcctgtg ctacccggcc gcgcgatacc ttatgggcag
     5221 catgaccccc caggccgtgc tggcgttcgt ggccctcatc ccgccgacct tgcccggcac
     5281 aaacatcgtg ttgggggccc ttccggagga cagacacatc gaccgcctgg ccaaacgcca
     5341 gcgccccggc gagcggcttg acctggctat gctggccgcg attcgccgcg tttacgggct
     5401 gcttgccaat acggtgcggt atctgcaggg cggcgggtcg tggcgggagg attggggaca
     5461 gctttcgggg acggccgtgc cgccccaggg tgccgagccc cagagcaacg cgggcccacg
     5521 accccatatc ggggacacgt tatttaccct gtttcgggcc cccgagttgc tggcccccaa
     5581 cggcgacctg tacaacgtgt ttgcctgggc cttggacgtc ttggccaaac gcctccgtcc
     5641 catgcacgtc tttatcctgg attacgacca atcgcccgcc ggctgccggg acgccctgct
     5701 gcaacttacc tccgggatgg tccagaccca cgtcaccacc cccggctcca taccgacgat
     5761 ctgcgacctg gcgcgcacgt ttgcccggga gatgggggag gctaactgag ctctagagct
     5821 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5881 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5941 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6001 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6061 gcttctgagg cggaaagaac cagctggggc tcgacatact agagtactta actttaaata
     6121 atgccaatta tttaaagtta ggcgcgccga tatctagggt tagagtccat ttacaaagct
     6181 gccaggaaac cggccacttc tagtaaacca cgtcgtgcct cactgggtcc tgcttacctc
     6241 atttctgaat gtgcatttcc agccttcttg ctctcagagc tattgttcaa gcagaaaaca
     6301 agctgctttt attacagtat gatgtcatga ctcatttgta acagatccag cctcagggac
     6361 agccctgtaa ggcagcaagt ggggctggct ccaaatgggt atgagtctca gaatctttgg
     6421 taaggcagaa ctgaactggg ctgagaggtg gtcttaaggc ctgggcaggc tctattctct
     6481 ctggactggc tgcagcctgc agtctaggag aggcccagta cagcctggag ctcctgagcc
     6541 ttgtcaacag gcagtgagcc cagagctgct tgaaagctgt cggtgtgctg tttggccagg
     6601 aacgtcagga gcagcaacag tgcggccttg gtgtctgggt ggagagggag ggagaaaggt
     6661 cggggctcct aaagcctcgc tgccccaggc tctctgagcc ctgccactcc cagcctcccg
     6721 caagcccctg cctatgactc ctaccctcac ctggtgggat cttgttgtca gccagaatga
     6781 ggctgcagat acgcagaagc tcgggagcca catctataac ctgtgaggaa agagtggctg
     6841 actcagggca gcagccccag accccaggtc taggagggtg gtcaggacca aggagtggca
     6901 gaatctctta tcaggggggt ggttgcacct ggtactgatt cacaggcatc caagacaact
     6961 atgaacatga gaagaaccaa tgaaacttgg tgctaatgat tttttttgga gggaatgggg
     7021 gatgtgggac aagatgatta aagtcatagc ctcaagacct aaagaaaccc agacactcag
     7081 gagctccacc tacacaacag tctctaaagg tataagacaa gggtgacaca aacactctga
     7141 catgactcca aacctagcta cttggtacag gcaaaatctc ttcagagagc aacctccaga
     7201 tcttgtcagt gtggttttca agaggaagca aaaagcctct ccacccaggc ctggaatgtt
     7261 tccacccaat gtcgagcaaa ccccgcccag cgtcttgtca ttggcgaatt cgaacacgca
     7321 gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga cgcgtgtggc
     7381 ctcgaacacc gagcgaccct gcaggtcctc gccatggatc ctgatgatgt tgttgattct
     7441 tctaaatctt ttgtgatgga aaacttttct tcgtaccacg ggactaaacc tggttatgta
     7501 gattccattc aaaaaggtat acaaaagcca aaatctggta cacaaggaaa ttatgacgat
     7561 gattggaaag ggttttatag taccgacaat aaatacgacg ctgcgggata ctctgtagat
     7621 aatgaaaacc cgctctctgg aaaagctgga ggcgtggtca aagtgacgta tccaggactg
     7681 acgaaggttc tcgcactaaa agtggataat gccgaaacta ttaagaaaga gttaggttta
     7741 agtctcactg aaccgttgat ggagcaagtc ggaacggaag agtttatcaa aaggttcggt
     7801 gatggtgctt cgcgtgtagt gctcagcctt cccttcgctg aggggagttc tagcgttgaa
     7861 tatattaata actgggaaca ggcgaaagcg ttaagcgtag aacttgagat taattttgaa
     7921 acccgtggaa aacgtggcca agatgcgatg tatgagtata tggctcaagc ctgtgcagga
     7981 aatcgtgtca ggcgatctct ttgtgaagga accttacttc tgtggtgtga cataattgga
     8041 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     8101 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     8161 atgggagcag tggtggaatg cagatccact agagtcgagg gatgcagaaa ttgatgatct
     8221 attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag
     8281 ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg
     8341 ggtgggatta gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa
     8401 tgtttcatag ttggatgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8461 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8521 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8581 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8641 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8701 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8761 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8821 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8881 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8941 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9001 gatctcaa
//
