LOCUS       sequence-439873-        9572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNA plasmid against human SRGAP2A gene with EGFP expression in 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9572)
  AUTHORS   Libe-Philippot B, Iwata R, Recupero AJ, Wierda K, Bernal Garcia S, 
            Hammond L, van Benthem A, Limame R, Ditkowska M, Beckers S, 
            Gaspariunaite V, Peze-Heidsieck E, Remans D, Charrier C, Theys T, 
            Polleux F, Vanderhaeghen P
  TITLE     Synaptic neoteny of human cortical neurons requires species-specific
            balancing of SRGAP2-SYNGAP1 cross-inhibition.
  JOURNAL   Neuron. 2024 Oct 9:S0896-6273(24)00645-7. doi: 
            10.1016/j.neuron.2024.08.021.
  PUBMED    39406239
REFERENCE   2  (bases 1 to 9572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2024 Oct 9:S0896-6273(24)00645-7. doi: 
            10.1016/j.neuron.2024.08.021."
COMMENT     Sequence Label: pLV-H1-shSRGAP2A-hsynapsin-EGFP
FEATURES             Location/Qualifiers
     source          1..9572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(73..287)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(133..152)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        314..761
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      776..785
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(782..784,785..787,788..1501)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(827..848)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1088..1107)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1435..1456
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1535..2123
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1588..1608)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2006..2017)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2126..2142)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             2652..2832
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2858..2875)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2864..3088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3134..3562
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3221..3240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3431..3452
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3571..3591)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3576..3905
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3756..3891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3818..3837
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3953..4000
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4019..4393
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4523..4644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4560..4579)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4614..4633
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4693..4709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4717..4733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4741..4747,4748..4765,4766..4771))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4786..4807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4924..4941)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5095..5683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5175..5194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5854..6645,6646..6714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6477..6496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6715..6819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6894..6913)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7085..7464
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7466..7664
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7619..7639
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7682..7862
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7909..8034
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8527..8760
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8945..8989
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9287..9404
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9476..9481,9482..9499,9500..9506)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9514..9530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9538..9554
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagcaaaaa agccactcat ccctgaagaa tcgtgacagg aagcgattct tcagggatga
       61 gtggcgggat ccgtggtctc atacagaact tataagattc ccaaatccaa agacatttca
      121 cgtttatggt gatttcccag aacacatagc gacatgcaaa tattgcaggg cgccactccc
      181 ctgtccctca cagccatctt cctgccaggg cgcacgcgcg ctgggtgttc ccgcctagtg
      241 acactgggcc cgcgattcct tggagcgggt tgatgacgtc agcgttcgaa ttctgcagag
      301 ggccctgcgt atgagtgcaa gtgggtttta ggaccaggat gaggcggggt gggggtgcct
      361 acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc caaattgcgc
      421 atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc gcactgccag
      481 cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac cgccgcctca
      541 gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt cccggccacc
      601 ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg cgcgagatag
      661 gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg cctcagtctg
      721 cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gtcgagaaac cggtcgccac
      781 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      841 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      901 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      961 cctcgtgacc accttgacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     1021 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1081 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1141 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1201 caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa
     1261 cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc
     1321 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1381 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     1441 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     1501 agtacaagta aaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     1561 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1621 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     1681 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     1741 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     1801 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     1861 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     1921 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     1981 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2041 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2101 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgctcgagac ctagaaaaac
     2161 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     2221 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     2281 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     2341 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     2401 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     2461 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     2521 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     2581 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     2641 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     2701 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     2761 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     2821 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2881 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2941 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3001 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     3061 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     3121 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3181 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3241 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3301 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3361 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3421 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     3481 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     3541 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3601 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3661 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3721 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3781 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3841 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3901 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     3961 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     4021 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     4081 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     4141 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     4201 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     4261 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     4321 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     4381 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     4441 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4501 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     4561 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     4621 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     4681 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4741 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4801 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4861 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4921 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4981 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5521 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5761 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5821 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5881 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5941 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6001 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6061 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6121 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6181 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6241 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6301 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6361 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6421 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6481 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6541 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6601 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6661 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6721 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6781 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6841 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     6901 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     6961 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     7021 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     7081 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     7141 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     7201 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     7261 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     7321 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     7381 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     7441 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     7501 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     7561 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     7621 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac
     7681 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7741 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7801 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7861 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7921 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7981 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8041 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8101 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8161 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8221 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8281 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8341 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8401 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8461 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8521 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8581 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8641 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8701 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8761 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8821 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8881 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     8941 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9001 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9061 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9121 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     9181 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
     9241 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9301 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     9361 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     9421 acagcagaga tccagtttgg ttaatgagtt agctcactca ttaggcaccc caggctttac
     9481 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     9541 gaaacagcta tgaccatgat tacgccaagc tg
//
