LOCUS       sequence-439875-        9576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNA plasmid against human SRGAP2B/C genes (#2) with EGFP 
            expression in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   Libe-Philippot B, Iwata R, Recupero AJ, Wierda K, Bernal Garcia S, 
            Hammond L, van Benthem A, Limame R, Ditkowska M, Beckers S, 
            Gaspariunaite V, Peze-Heidsieck E, Remans D, Charrier C, Theys T, 
            Polleux F, Vanderhaeghen P
  TITLE     Synaptic neoteny of human cortical neurons requires species-specific
            balancing of SRGAP2-SYNGAP1 cross-inhibition.
  JOURNAL   Neuron. 2024 Oct 9:S0896-6273(24)00645-7. doi: 
            10.1016/j.neuron.2024.08.021.
  PUBMED    39406239
REFERENCE   2  (bases 1 to 9576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2024 Oct 9:S0896-6273(24)00645-7. doi: 
            10.1016/j.neuron.2024.08.021."
COMMENT     Sequence Label: pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP
FEATURES             Location/Qualifiers
     source          1..9576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(77..291)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(137..156)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        318..765
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      780..789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(786..788,789..791,792..1505)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(831..852)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1092..1111)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1439..1460
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1539..2127
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1592..1612)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2010..2021)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2130..2146)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             2656..2836
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2862..2879)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2868..3092
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3138..3566
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3225..3244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3435..3456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3575..3595)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3580..3909
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3760..3895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3822..3841
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3957..4004
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4023..4397
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4527..4648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4564..4583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4618..4637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4697..4713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4721..4737
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4745..4751,4752..4769,4770..4775))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4790..4811
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4928..4945)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5099..5687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5179..5198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5858..6649,6650..6718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6481..6500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6719..6823)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6898..6917)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7089..7468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7470..7668
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7623..7643
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7686..7866
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7913..8038
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8531..8764
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8949..8993
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9291..9408
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9480..9485,9486..9503,9504..9510)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9518..9534
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9542..9558
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctagcaaaaa aaaggacagg cattgaatat cttagtgaca ggaagctaag atattcaatg
       61 cctgtccttg ggatccgtgg tctcatacag aacttataag attcccaaat ccaaagacat
      121 ttcacgttta tggtgatttc ccagaacaca tagcgacatg caaatattgc agggcgccac
      181 tcccctgtcc ctcacagcca tcttcctgcc agggcgcacg cgcgctgggt gttcccgcct
      241 agtgacactg ggcccgcgat tccttggagc gggttgatga cgtcagcgtt cgaattctgc
      301 agagggccct gcgtatgagt gcaagtgggt tttaggacca ggatgaggcg gggtgggggt
      361 gcctacctga cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt
      421 gcgcatcccc tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg
      481 ccagcttcag caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc
      541 ctcagcactg aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc
      601 caccttggtc gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag
      661 ataggggggc acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag
      721 tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgag aaaccggtcg
      781 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
      841 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
      901 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
      961 ccaccctcgt gaccaccttg acctacggcg tgcagtgctt cgcccgctac cccgaccaca
     1021 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1081 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1141 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1201 ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc gacaagcaga
     1261 agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc agcgtgcagc
     1321 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1381 accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca
     1441 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1501 agtaagtaca agtaaaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     1561 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     1621 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     1681 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     1741 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     1801 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     1861 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     1921 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     1981 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     2041 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     2101 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgctcg agacctagaa
     2161 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     2221 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     2281 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     2341 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     2401 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     2461 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     2521 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     2581 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     2641 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2701 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2761 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2821 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     2881 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     2941 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     3001 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     3061 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     3121 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3181 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3241 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3301 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     3361 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3421 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     3481 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3541 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     3601 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3661 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3721 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3781 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3841 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3901 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     3961 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     4021 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     4081 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     4141 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     4201 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     4261 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     4321 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     4381 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     4441 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     4501 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     4561 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4621 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     4681 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4741 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4801 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4861 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4921 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4981 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     5041 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5101 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5521 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5701 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5761 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5821 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5941 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6001 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6841 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     6901 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     6961 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     7021 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     7081 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     7141 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     7201 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     7261 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     7321 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     7381 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     7441 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     7501 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     7561 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     7621 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct
     7681 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7741 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7801 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7861 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     7921 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     7981 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     8041 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     8101 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     8161 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     8221 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     8281 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     8341 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     8401 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     8461 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     8521 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     8581 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     8641 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     8701 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     8761 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     8821 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     8881 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     8941 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     9001 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     9061 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     9121 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     9181 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     9241 cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga
     9301 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     9361 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     9421 agggacagca gagatccagt ttggttaatg agttagctca ctcattaggc accccaggct
     9481 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9541 acaggaaaca gctatgacca tgattacgcc aagctg
//
