LOCUS       sequence-439882-        8659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            DDX4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-DDX4
FEATURES             Location/Qualifiers
     source          1..8659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    52..70
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     277..301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    367..391
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      392..401
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2573..2597)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2599..2625
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2792..2926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3198..3232
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3243..3260)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3243..3259)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3252..3274)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3468..3487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3589..3611
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3650..3668)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3736..3840
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3841..3909,3910..4701)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4059..4078)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4872..5460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5361..5380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5614..5631
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5837..5858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5873..5878,5879..5896,5897..5903)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5911..5927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5916..5938
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5935..5951
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5935..5951
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5977..6039
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6215..6296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7013..7069)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7079..7822)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7823..7846)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7846..7855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7875..8086)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        8087..8466
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcacctcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
       61 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
      121 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
      181 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
      241 gtgatagaga acgtataagc ttgcctatat aagcagagct cgtttagtga accgtcagat
      301 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      361 cctaccacaa gtttgtacaa aaaagcaggc tgccaccatg ggagatgaag attgggaagc
      421 agaaatcaac cctcatatgt cttcctatgt tcccatattt gagaaggata ggtattctgg
      481 agaaaatgga gacaatttta acaggactcc agcttcatca tcagaaatgg atgatggacc
      541 ttctcgaaga gatcatttca tgaaaagtgg atttgcctct gggcggaatt ttggaaacag
      601 agatgctggt gagtgtaata agcgagataa tacatccaca atgggtggtt ttggagttgg
      661 aaagagtttt ggaaacagag gtttttcaaa cagcaggttt gaagatggtg atagctctgg
      721 tttctggaga gagtctagta atgactgcga agataatcca acacggaaca gagggttttc
      781 caagagaggc ggctatcgag atggaaataa ttcagaagct tcagggccat acagaagagg
      841 tggaagaggt agtttccgag gttgccgtgg aggatttggt ctaggaagtc caaataatga
      901 cttagaccca gacgaatgta tgcagcgcac tggtggcctt tttggttcta gaagaccagt
      961 attaagtggc acaggtaatg gtgatacttc tcaaagcaga agtggcagtg gaagtgaacg
     1021 aggtggttac aaaggtttaa atgaagaagt aataacaggc tctggaaaga attcttggaa
     1081 gtcagaagca gaaggaggag aaagtagtga tactcaagga ccaaaagtga cctacatacc
     1141 ccctcctcca cctgaggatg aggactccat ctttgcacat tatcagacag gcataaactt
     1201 cgacaaatac gacactattc ttgtggaagt gtctggacat gatgcaccac cagcaattct
     1261 gacttttgaa gaagctaatc tctgtcagac actgaataac aacattgcta aagctggtta
     1321 tactaagctt actcctgtgc aaaaatacag tattcctatc atacttgcag gacgagattt
     1381 gatggcttgc gctcaaacag ggtctgggaa gactgcggct tttctcctac caattttggc
     1441 tcatatgatg catgatggaa taactgccag tcgttttaaa gagttgcagg aaccagagtg
     1501 tattattgta gcaccaactc gagaattggt caaccagatt tatttggaag ccagaaaatt
     1561 ttcttttggg acttgtgtaa gagctgttgt tatatatggg ggaacccagc tgggacattc
     1621 aattcgacaa atagtacaag gctgtaatat attatgtgct actcctggaa gactgatgga
     1681 tatcataggc aaagaaaaga ttggtctcaa acagatcaaa tacttagttt tggatgaagc
     1741 tgatcgcatg ttggatatgg gttttggtcc agaaatgaag aagttaattt cttgcccagg
     1801 aatgccatca aaggaacagc gccaaaccct tatgttcagt gcaacttttc cagaggaaat
     1861 tcaaaggttg gctgcagagt ttttaaagtc aaattatctg tttgttgctg ttggacaagt
     1921 gggtggagca tgtagagatg ttcagcagac cgttctccaa gttggccagt tctcaaaaag
     1981 agaaaagctc gttgaaattc tgcgaaacat aggggatgaa agaactatgg tctttgttga
     2041 aactaagaaa aaagcagatt ttattgcaac ttttctttgt caagaaaaaa tatcaactac
     2101 aagtattcat ggtgatcggg aacagagaga gcgggagcaa gctcttggag attttcgctt
     2161 tggaaagtgc ccagttcttg ttgctacttc agtagctgcc agagggctgg atattgaaaa
     2221 tgtgcaacat gttatcaatt ttgatcttcc ttctaccatt gatgaatatg ttcatcgaat
     2281 tgggcgtact ggtcgttgtg ggaatactgg cagagcaatt tccttttttg atcttgaatc
     2341 ggataaccat ttagcacagc ctctagtaaa agtattgaca gatgctcaac aggatgttcc
     2401 tgcatggttg gaagaaattg cctttagtac atacattcct ggcttcagtg gtagtacaag
     2461 aggaaacgtg tttgcatcag ttgataccag aaagggcaag agcactttga acacagctgg
     2521 gttttcttct tcacaagctc ccaatccagt agatgatgag tcatgggatt aaacccagct
     2581 ttcttgtaca aagtggtata cccatacgat gttccagatt acgcttagta atgagaaata
     2641 ctcagaagat gtcactcacc atagtccgca gtgaaagata aatgatccat aagcttcctt
     2701 gtcctacgtt ttagagctag aaatagcaag taggccactg agcaagaact atgaatgaga
     2761 tctttagaca tattgcggcc gctttctccg cgatccagac atgataagat acattgatga
     2821 gtttggacaa accaaaacta gaatgcagtg aaaaaaatgc cttatttgtg aaatttgtga
     2881 tgctattgcc ttatttgtaa ccattataag ctgcaataaa caagttatct ataacaagaa
     2941 aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta
     3001 tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc
     3061 gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca
     3121 agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga
     3181 gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaagaat
     3241 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3301 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3361 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     3421 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     3481 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3541 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccatgtctcc gggagctgca
     3601 tgtgtcagag gttttcaccg tcatcaccga aacacgcgag ttcgaaaggg cctcgtgata
     3661 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3721 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3781 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3841 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3901 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3961 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4021 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4081 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4141 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4201 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4261 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4321 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4381 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4441 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4501 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggttca
     4561 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4621 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4681 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4741 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4801 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4861 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4921 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4981 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5041 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5101 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5161 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5221 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5281 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5341 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5401 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5461 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5521 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5581 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggat
     5641 gagcgcccaa tacgcaaacc gcctctcacc gcgcgttggc cgattcatta atgcagctgg
     5701 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaaaccg cctctccccg
     5761 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     5821 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     5881 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     5941 acagctatga ccatgattac gccaaggtcg acttaaccct agaaagataa tcatattgtg
     6001 acgtacgtta aagataatca tgcgtaaaat tgacgcatgt gttttatcgg tctgtatatc
     6061 gaggtttatt tattaatttg aatagatatt aagttttatt atatttacac ttacatacta
     6121 ataataaatt caacaaacaa tttatttatg tttatttatt tattaaaaaa aaacaaaaac
     6181 tcaaaatttc ttctataaag taacaaaact tttagcagtg aaaaaaatgc tttatttgtg
     6241 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     6301 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     6361 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctggag attcatgcgc
     6421 caggttttct ggtcatgcac cacgttcgtg ggccctcagg aacctccacg tcagcggtaa
     6481 ctgtgaagcc cagtcgctcg taaaaaggaa gattccgggg agcactggtt tcgaggaacg
     6541 ccggcacgcc agcccgttcc gcggcctcaa caccaggaag aacgacagcg cttcccaaac
     6601 ctttaccttg gtgatctggg ctgacaccga cagtggcaag aaaccacgca ggctcctttg
     6661 gtctatgagg agccagcaag ccctccattt gctgttgtgc cgccaaccta ctccctgaca
     6721 gctcggccat ccgcggacct atttccgcga acactgcgcc ggcttcgacg ctttccggag
     6781 tagtccagac cgcaactgcg gcaccatcgt ccgcaaccca aactttacca atgtccagac
     6841 ccacgcgcgt aagaaacagc tcttgcaact cagttaccct ctctatgtgt ctgtccggat
     6901 ctacggtatg gcgggtcgct ggataatctg caaatgctgc ggcaagtgtt cggacagccc
     6961 gtggtacatc gtcacgggta gcgagcctaa cagtgggctt atattccgtc atggggccgg
     7021 ggttctcctc cacgtcgccg gcctgcttca gcaggctgaa gttggtggcg ccgctgcccc
     7081 cggggagcat gtcaaggtca aaatcgtcaa gagcgtcagc aggcagcata tcaaggtcaa
     7141 agtcgtcaag ggcatcggct gggagcatgt ctaagtcaaa atcgtcaagg gcgtcggtcg
     7201 gcccgccgct ttcgcacttt agctgtttct ccaggccaca tatgattagt tccaggccga
     7261 aaaggaaggc aggttcggct ccctgccggt cgaacagctc aattgcttgt ttcagaagtg
     7321 ggggcataga atcggtggta ggtgtctctc tttcctcttt tgctacttga tgctcctgtt
     7381 cctccaatac gcagcccagt gtaaagtggc ccacggcgga cagagcgtac agtgcgttct
     7441 ccagggagaa gccttgctga cacaggaacg cgagctgatt ttccagggtt tcgtactgtt
     7501 tctctgttgg gcgggtgccg agatgcactt tagccccgtc acgatgtgag aggagagcac
     7561 agcggtatga cttggcgttg ttccgcagaa agtcttgcca tgactcgcct tccagggggc
     7621 aggagtgggt atgatgcctg tccagcatct cgattggcag ggcatcgagc agggcccgct
     7681 tgttcttcac gtgccagtac agggtaggct gctcaactcc cagcttttga gcgagtttcc
     7741 ttgtcgtcag gccttcgata ccgactccat tgagtaattc cagagcagag tttatgactt
     7801 tgctcttgtc cagtctagac atcttatcgt catcgtcttt gtaatccatg gtggcggatc
     7861 ccgcgtcacg acacctgtgt tctggcggca aacccgttgc gaaaaagaac gttcacggcg
     7921 actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag ccagtacacg
     7981 acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca attgccgacc
     8041 cctcccccca acttctcggg cactgtgggc catgtgcgct ctgccccatg gtaatagcca
     8101 tgactaatac gtagatgtac tgccaagtag gaaagtgcca taaggtcatg tactgggcat
     8161 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     8221 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     8281 ggaaagtgcc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     8341 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     8401 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
     8461 aatgtcataa gctctaatcg cataatcgag ctaagagcga ttaatatgct tagctagctt
     8521 agctaagcta gatttctcta ttctcccaca atatatagca gctattagct tagcagtccg
     8581 aatacgatcg tccacgatcc ataagcgtaa cacatttgag tagcgactga cgggcaccgg
     8641 agccactcga gtggaatta
//
