LOCUS       sequence-439892-        4481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for inserting GFP11-HA to the end of Drosophila 5-HT7R
            coding frame.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Mallick A, Tan HL, Epstein JM, Gaudry Q, Dacks AM
  TITLE     Serotonin acts through multiple cellular targets during an olfactory
            critical period.
  JOURNAL   bioRxiv [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413.
  PUBMED    38645269
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Apr 14:2024.04.14.589413. doi: 
            10.1101/2024.04.14.589413."
COMMENT     Sequence Label: pTwist Amp_d5-HT7R-sfGFP11-HA-CRISPR-HDR-donor
FEATURES             Location/Qualifiers
     source          1..4481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(929..946)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(929..945)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(938..960)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1059..1163
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1164..1232,1233..2024)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSATITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1382..1401)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    2124..2148
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      2265..2853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2754..2773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3006..3028
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3025..3041
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3025..3041
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(4058..4084)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(4088..4135)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 gtaaaggccg acatgatgat gcccagagtg gtggaggcct tcttctcttt ggccagttga
       61 aaccgcagct tcttgtggtg cggagagccc aggagtccgg tcgagccact aacgccaccc
      121 ccactgccgt gcccagccag tgcgcctcct ccgctgctca gacccccaca ggtggagtag
      181 gtgagcgaag tgttgcccac gctgctgtgc ctctggccat ttcccgagct ggccagctcc
      241 gtgtggccca agggcggagc ctgcggggcc gatggcgatc ctgtgccatt gagcgcctgt
      301 tgcaaatgcg tctgggcgcg tttctcttcc aggacaatcc gcctcgccgc cctgaagatc
      361 tggtagtaca caaacagcat caccgagagt ggtatgtaaa atgagcccag ggtggcgtag
      421 atctgatatg cgaagttctg gcacaccgtg cagataggct ggccctcctc gtcctcgtgc
      481 tcgttgccca ggatcagcag aggaggcaat gagatgcagg cggcggccag ccagacgatg
      541 cccacgcaca gcatcattcg ccgaggagtc ctcttcacgc cgtactccag gggcttcgtg
      601 atggccaggt atcgatccac ggaaatggca cacagattca gaatcgaggc cgtgcagcac
      661 agcacatcga aggacaccca gatgtcgcag agcagcggtc caaagttcca cttctccagc
      721 acttcataaa gcaaggccat gggcatcacc agaagagcca cacagagatc cgaaagagcc
      781 aaggacacca aaaggtaatt gcagggtctc ctcaatttcc gcaccatgca aacggctatg
      841 cagaccagga cattgcccac tacagtggct agcataatta ggtcaggcgg aatggcactt
      901 ccttccagat gcgtgcggat tatgcatgac tggccgtcgt tttacaacgt cgtgactggg
      961 aaaaccctgg cgcttgtggt ggatcacagt tattggagac ttccttggcc gtacgatggg
     1021 ttttgtttca ggtatctggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     1081 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     1141 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     1201 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     1261 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     1321 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     1381 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     1441 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     1501 atggcatgac agtaagagaa ttatgcagtg ctaccataac catgagtgat aacactgcgg
     1561 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1621 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1681 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1741 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1801 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1861 ctggagccgg tgagcgtggc tctcgcggta tcattgcagc actggggcca gatggtaagc
     1921 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1981 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     2041 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2101 agatccttga attgccatta tcaacaagtt tgtacaaaaa agcaggctcc gaattggtat
     2161 cacgaggacg atttgaagcc ccctacccat ttacgctgac ttgacgggac ggcggctttg
     2221 ttgaatagag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2881 ttcctggcct tttgctggcc ttttgctcac atgttctttc cagcgacata tgccatacgc
     2941 cgaactgaac tccacctaat gtcttggaga ttgtcggata gcgcggaata ggattgtgcc
     3001 ctctgagcgg ataacaattt cacacaggaa acagctatga ccatgacaca tgcatccacc
     3061 atcgcagact tatcatcact gagcctccac ctagcctata attgcggccg cagggggctg
     3121 ggtatttaca gtggatagct tcttgctcaa aacgtaaata tatctaggtt aatcagtagg
     3181 cttaatttgg ctgcaatctg cagtttgtaa atttaatttt tggctcagtt tgagtatcgc
     3241 aaatcaacta actacgctgg caaattttgg gatggggact gtgggatctt tttcttgttc
     3301 tgggaggtat caatagattc gataacgatt gtgtctcata tccggtgctg attgttttcg
     3361 aaacataaaa ggaaccgtag aaacctgtca cagttacaac cgtgtgtgtg tgtgtgtgta
     3421 tgattggttg gtgagtgtga ttgtgtgcgt ggcacgcacc acgtcgattg cttagttcta
     3481 catttaaaat tacaacaaca agttacattt agaggagtcg tctacaattc gcacattgct
     3541 gcaagtttca gggggtgcac atatacagag atacgctacg ctactagcac tactacatac
     3601 aaatatccat gtgtgtgtac tggggtggtg gcaatatgcg gggaaaggta atatttgctc
     3661 tgctttccgt acgttttctt ttgagtcaat ggggggtctg caatcaatgt aacccttata
     3721 aacatatcgt taaatttaat agaaaattaa tttatagaca attaacggac atgatcaaac
     3781 tctcagttac aaataatatt agatgattat ttctgctaaa taaactatac caacgcggta
     3841 ctttaaacag cccagagcct acttttccca cttaaaactt tacatttcct aattgtttaa
     3901 gatgtacacc ccagtgttta tacctaagtg tgtgagtgtg tcgagcagag gagtcgcctt
     3961 cttccggaaa cctagggaag acccttcgag cagctcagat tcgcttggat aggctccttt
     4021 ctccggaatg gaggtcctcg tggatggtct agagttaagc gtaatctgga acatcgtatg
     4081 ggtaactagt gatgccggcg gcgttcacgt actcgtgcag caccatgtgg tcccggctgc
     4141 ctcctccgct gcctccgtcg cctccgcttc ctccatcgcc gagaaagctc tccctcgccc
     4201 cgtgcctctc atcgcccagc atcacccgct gcgagggagg ctcgccatac tgatcctggt
     4261 agtagttctc ccgcatcatg gtgttcaggc tggagcagcg gaagtagagg atctcctgga
     4321 agggtttgcg gaaatccctg ttcagagtgg cgtagataat agggttcagc agggagttgg
     4381 catagcccag ccagaggaat agagacgaga gcgatgccgg tacgtgcatt gtctcgaaag
     4441 gacgaattag ggccaggata aagaatggca gccagcagac g
//
