LOCUS       sequence-439906-       10309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            SYMPK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10309)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 10309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-SYMPK
FEATURES             Location/Qualifiers
     source          1..10309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    53..71
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    89..107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    197..215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    233..251
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     278..302
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    368..392
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      393..402
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4224..4248)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4250..4276
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4443..4577
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4849..4883
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4894..4911)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4894..4910)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4903..4925)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5119..5138)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5240..5262
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5301..5319)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5387..5491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5492..5560,5561..6352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5710..5729)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6523..7111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7012..7031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7265..7282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7488..7509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7524..7529,7530..7547,7548..7554)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7562..7578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7567..7589
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7586..7602
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7586..7602
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   7628..7690
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    7866..7947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8664..8720)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(8730..9473)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(9474..9497)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9497..9506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9526..9737)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        9738..10117
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag cttgcctata taagcagagc tcgtttagtg aaccgtcaga
      301 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      361 gcctaccaca agtttgtaca aaaaagcagg ctgccaccat ggcgagcggc agtggagaca
      421 gcgtcacccg tcggagcgtg gcatcacagt ttttcactca agaggagggg ccgggcatcg
      481 atggcatgac cacctcagag agggtggtgg atcttctgaa ccaggcggcg ctgatcacca
      541 atgactcaaa gatcacagtg ctcaaacagg tccaggagct gatcatcaac aaagacccca
      601 cactactgga caacttcctg gatgagatca tcgcattcca agcagacaag tcaatcgaag
      661 tgcgaaaatt tgtcatcggc ttcatcgagg aggcatgcaa gcgagacatt gagttgctgc
      721 tgaaactcat tgcaaacctc aacatgctct tgagggacga gaatgtgaac gtggtgaaga
      781 aggctatcct caccatgacc cagctctaca aggtggccct gcagtggatg gtaaagtcac
      841 gggtcattag cgagctacag gaggcctgct gggacatggt atctgccatg gcgggggaca
      901 tcatcctgct attggactct gacaatgacg gcatccgcac ccacgccatc aagtttgtgg
      961 agggcctcat tgtcaccctg tcaccccgca tggctgactc agagataccc cgacgccagg
     1021 agcatgatat cagcctggac cgcatccctc gtgaccaccc ctacatccag tacaacgtgc
     1081 tatgggaaga gggcaaggca gccttggagc agctgcttaa gttcatggtg caccctgcca
     1141 tctcctccat caacctgacc acagcgctgg gctcccttgc caatatcgcc cgccagagac
     1201 ccatgttcat gtctgaggtg atccaggcct atgaaactct gcatgccaac ctgcccccga
     1261 cgctggccaa atcgcaggtg agcagtgtgc gtaagaatct gaagctgcac ctgttgagtg
     1321 tgctgaagca cccggcttcc ttggagttcc aggcccagat caccaccctg ctggtggacc
     1381 tgggcacacc tcaggccgag atcgcccgca acatgccgag cagcaaggac acccgcaagc
     1441 ggccccgcga tgactcggac tccacactca agaagatgaa gctggagccc aacctggggg
     1501 aggacgatga ggacaaagac ttggagccag gcccgtcggg gacctcgaag gcctcagcgc
     1561 agatctccgg ccagtcagac acggacatca cagctgagtt cctgcagcct ctgctgacgc
     1621 ctgataatgt ggctaatctg gtcctcatca gcatggtgta cctacccgag gccatgccag
     1681 cctccttcca ggccatctac acccccgtgg agtcagcagg cacggaagcc cagatcaagc
     1741 acctggctcg gctcatggcc acacagatga cagctgccgg actgggacca ggtgtagagc
     1801 agaccaaaca gtgcaaggag gagcccaagg aggagaaggt ggtgaagaca gagagcgtcc
     1861 tgatcaagcg gcgcctgtca gcccagggcc aagccatctc ggtggtgggt tccctgagct
     1921 ccatgtcccc cctggaggaa gaggcaccgc aggccaagag gaggccagag cccattatcc
     1981 ctgtcactca gccccggctg gcaggcgctg gtgggcgcaa gaaaattttc cgtctcagcg
     2041 acgtgctgaa gccccttacc gatgcccagg tggaagccat gaagctgggc gctgtgaagc
     2101 ggatcctgcg ggctgagaag gctgtggcct gcagcggggc agcccaggtc cgcataaaga
     2161 tcctggccag cctggtgaca cagttcaact cgggcctgaa ggcggaggtc ctgtccttca
     2221 tcctggagga tgtgcgggcc cgcctggacc tggccttcgc ctggctctac caggagtaca
     2281 acgcctacct ggccgcaggt gcctcgggct ccctggacaa gtatgaggac tgcctcatcc
     2341 gcctgttgtc tggcctgcag gagaaaccag accagaagga tgggatcttc accaaggttg
     2401 tgctggaggc gccactcatc acagagagtg ccctggaggt ggtccgcaag tactgcgagg
     2461 atgagagtcg cacctatctg ggcatgtcca cacttcgaga cctgatcttc aagcgcccgt
     2521 cccgccagtt ccagtacctg catgtcctcc tcgacctcag ctcccatgag aaggacaagg
     2581 tgcgctccca ggccctgctg ttcatcaaac gcatgtatga gaaggagcag ctgcgggagt
     2641 atgtggagaa atttgccctc aactacctgc agctcctggt gcaccccaac ccaccgtctg
     2701 tgctgtttgg agctgacaag gacacagagg tggcagcacc ctggacggag gagacagtga
     2761 agcagtgtct gtacctctac ctggccctcc tgcctcagaa ccacaagctg atccacgaac
     2821 tggcggccgt gtacactgaa gccatcgccg acatcaagcg gacggtgctg agggtcattg
     2881 agcagccgat ccgaggaatg ggcatgaact ccccggagct gctcctgctg gtggaaaatt
     2941 gtcccaaggg agcagagaca ctggtcacga gatgtctgca cagcctcaca gacaaagtcc
     3001 caccctcccc agagctggtg aagcgggtcc gggatctcta ccacaagcga ctgccagacg
     3061 tccgcttcct catcccggtg ctcaatgggc tggagaagaa agaggtgatc caggccctgc
     3121 ctaaactcat caaactcaac cccatcgtgg tgaaggaagt cttcaaccgc ctgctgggca
     3181 cccagcatgg tgagggaaac tcagccttgt ccccgctgaa ccctggagag ctcctgatcg
     3241 cattacacaa cattgactcc gtgaagtgcg acatgaaatc catcatcaaa gccaccaacc
     3301 tgtgctttgc ggagcggaac gtgtacacgt cagaggtgct ggccgtggtg atgcagcagc
     3361 tgatggagca gagccccctg cccatgctgc tcatgaggac cgtcatccag tccctgacca
     3421 tgtacccccg cctggggggc ttcgtcatga acatcctgtc ccgcctcatc atgaagcagg
     3481 tgtggaagta ccccaaggtg tgggagggct tcatcaagtg ctgccagcgc acaaagcccc
     3541 agagcttcca ggtcatcctg cagctgccgc cccagcagct gggagccgtc tttgacaagt
     3601 gcccagagct ccgggagccc ctgctggccc atgtccgctc cttcaccccc caccagcaag
     3661 ctcacatccc taactccatc atgaccatct tggaggccag cggcaagcag gagccagagg
     3721 ccaaggaggc gcctgcgggg cccttggagg aggatgatct ggagcccctg accttggccc
     3781 cggccccagc accccggccc cctcaggacc tcatcggcct gcgactggcc caggagaagg
     3841 ccttaaagcg gcagctggag gaggaacaga agctgaagcc gggaggagtg ggagccccct
     3901 cctcttcctc cccctctccc tctccgtcgg cccggccagg cccgcccccg tctgaggaag
     3961 ccatggattt ccgggaggag gggcctgagt gcgagacccc gggcatcttc atcagcatgg
     4021 atgacgactc ggggctgacc gaggccgcgc tgttggactc tagtctcgag ggccccctac
     4081 ccaaggagac ggcagcgggc gggctgacct tgaaggagga gcggagcccc cagaccctcg
     4141 cacctgttgg agaagatgct atgaagactc ccagcccggc tgccgaggac gccagggaac
     4201 ccgaggccaa ggggaacagc tgaacccagc tttcttgtac aaagtggtat acccatacga
     4261 tgttccagat tacgcttagt aatgagaaat actcagaaga tgtcactcac catagtccgc
     4321 agtgaaagat aaatgatcgg tgcagaagct aactttccgt tttagagcta gaaatagcaa
     4381 gtaggccact gagcaagaac tatgaatgag atctttagac atattgcggc cgctttctcc
     4441 gcgatccaga catgataaga tacattgatg agtttggaca aaccaaaact agaatgcagt
     4501 gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc cttatttgta accattataa
     4561 gctgcaataa acaagttatc tataacaaga aaatatatat ataataagtt atcacgtaag
     4621 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     4681 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     4741 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     4801 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     4861 ttacgcagac tatctttcta gggttaagaa ttcactggcc gtcgttttac aacgtcgtga
     4921 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4981 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5041 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     5101 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5161 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5221 acaagctgtg accatgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5281 aaacacgcga gttcgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5341 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5401 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5461 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5521 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5581 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5641 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5701 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5761 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5821 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5881 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5941 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6001 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6061 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6121 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6181 tgataaatct ggagccggtg agcgtggttc acgcggtatc attgcagcac tggggccaga
     6241 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6301 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6361 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6421 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7141 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7201 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7261 gcgcagcgag tcagtgagcg aggaagcgga tgagcgccca atacgcaaac cgcctctcac
     7321 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     7381 cagtgagcgc aacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7441 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7501 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7561 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaaggtc
     7621 gacttaaccc tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa
     7681 ttgacgcatg tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat
     7741 taagttttat tatatttaca cttacatact aataataaat tcaacaaaca atttatttat
     7801 gtttatttat ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac
     7861 ttttagcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     7921 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     7981 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     8041 gctgattatg atcctctgga gattcatgcg ccaggttttc tggtcatgca ccacgttcgt
     8101 gggccctcag gaacctccac gtcagcggta actgtgaagc ccagtcgctc gtaaaaagga
     8161 agattccggg gagcactggt ttcgaggaac gccggcacgc cagcccgttc cgcggcctca
     8221 acaccaggaa gaacgacagc gcttcccaaa cctttacctt ggtgatctgg gctgacaccg
     8281 acagtggcaa gaaaccacgc aggctccttt ggtctatgag gagccagcaa gccctccatt
     8341 tgctgttgtg ccgccaacct actccctgac agctcggcca tccgcggacc tatttccgcg
     8401 aacactgcgc cggcttcgac gctttccgga gtagtccaga ccgcaactgc ggcaccatcg
     8461 tccgcaaccc aaactttacc aatgtccaga cccacgcgcg taagaaacag ctcttgcaac
     8521 tcagttaccc tctctatgtg tctgtccgga tctacggtat ggcgggtcgc tggataatct
     8581 gcaaatgctg cggcaagtgt tcggacagcc cgtggtacat cgtcacgggt agcgagccta
     8641 acagtgggct tatattccgt catggggccg gggttctcct ccacgtcgcc ggcctgcttc
     8701 agcaggctga agttggtggc gccgctgccc ccggggagca tgtcaaggtc aaaatcgtca
     8761 agagcgtcag caggcagcat atcaaggtca aagtcgtcaa gggcatcggc tgggagcatg
     8821 tctaagtcaa aatcgtcaag ggcgtcggtc ggcccgccgc tttcgcactt tagctgtttc
     8881 tccaggccac atatgattag ttccaggccg aaaaggaagg caggttcggc tccctgccgg
     8941 tcgaacagct caattgcttg tttcagaagt gggggcatag aatcggtggt aggtgtctct
     9001 ctttcctctt ttgctacttg atgctcctgt tcctccaata cgcagcccag tgtaaagtgg
     9061 cccacggcgg acagagcgta cagtgcgttc tccagggaga agccttgctg acacaggaac
     9121 gcgagctgat tttccagggt ttcgtactgt ttctctgttg ggcgggtgcc gagatgcact
     9181 ttagccccgt cacgatgtga gaggagagca cagcggtatg acttggcgtt gttccgcaga
     9241 aagtcttgcc atgactcgcc ttccaggggg caggagtggg tatgatgcct gtccagcatc
     9301 tcgattggca gggcatcgag cagggcccgc ttgttcttca cgtgccagta cagggtaggc
     9361 tgctcaactc ccagcttttg agcgagtttc cttgtcgtca ggccttcgat accgactcca
     9421 ttgagtaatt ccagagcaga gtttatgact ttgctcttgt ccagtctaga catcttatcg
     9481 tcatcgtctt tgtaatccat ggtggcggat cccgcgtcac gacacctgtg ttctggcggc
     9541 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc
     9601 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca
     9661 ccttctctag gcaccggttc aattgccgac ccctcccccc aacttctcgg gcactgtggg
     9721 ccatgtgcgc tctgccccat ggtaatagcc atgactaata cgtagatgta ctgccaagta
     9781 ggaaagtgcc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     9841 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     9901 taccgtaaat actccaccca ttgacgtcaa tggaaagtgc ctattggcgt tactatggga
     9961 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
    10021 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
    10081 attgattact attaataact agtcaataat caatgtcata agctctaatc gcataatcga
    10141 gctaagagcg attaatatgc ttagctagct tagctaagct agatttctct attctcccac
    10201 aatatatagc agctattagc ttagcagtcc gaatacgatc gtccacgatc cataagcgta
    10261 acacatttga gtagcgactg acgggcaccg gagccactcg agtggaatt
//
