LOCUS       sequence-439908-        8731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac transposon plasmid for doxycycline inducible expression of 
            EZH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Pierson Smela M, Adams J, Ma C, Breimann L, Widocki U, Shioda T, 
            Church GM
  TITLE     Induction of Meiosis from Human Pluripotent Stem Cells
  JOURNAL   bioRxiv 2024.05.31.596483
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.31.596483"
COMMENT     Sequence Label: pPB-cT3G-cERP2-EZH2
FEATURES             Location/Qualifiers
     source          1..8731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    52..70
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    88..106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    124..142
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    160..178
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     277..301
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    367..391
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2645..2669)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2671..2697
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    2864..2998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3270..3304
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(3315..3332)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3315..3331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3324..3346)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3540..3559)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3661..3683
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3722..3740)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3808..3912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3913..3981,3982..4773)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4131..4150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4944..5532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5433..5452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5686..5703
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5909..5930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5945..5950,5951..5968,5969..5975)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5983..5999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5988..6010
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6007..6023
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6007..6023
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   6049..6111
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    6287..6368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7085..7141)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7151..7894)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(7895..7918)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      7918..7927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7947..8158)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     enhancer        8159..8538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcacctcgag tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca
       61 gtgatagaga acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt
      121 tactccctat cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac
      181 gtatctacag tttactccct atcagtgata gagaacgtat atccagttta ctccctatca
      241 gtgatagaga acgtataagc ttgcctatat aagcagagct cgtttagtga accgtcagat
      301 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      361 cctaccacaa gtttgtacaa aaaagttggc atgggccaga ctgggaagaa atctgagaag
      421 ggaccagttt gttggcggaa gcgtgtaaaa tcagagtaca tgcgactgag acagctcaag
      481 aggttcagac gagctgatga agtaaagagt atgtttagtt ccaatcgtca gaaaattttg
      541 gaaagaacgg aaatcttaaa ccaagaatgg aaacagcgaa ggatacagcc tgtgcacatc
      601 ctgacttctg tgagctcatt gcgcgggact agggagtgtt cggtgaccag tgacttggat
      661 tttccaacac aagtcatccc attaaagact ctgaatgcag ttgcttcagt acccataatg
      721 tattcttggt ctcccctaca gcagaatttt atggtggaag atgaaactgt tttacataac
      781 attccttata tgggagatga agttttagat caggatggta ctttcattga agaactaata
      841 aaaaattatg atgggaaagt acacggggat agagaatgtg ggtttataaa tgatgaaatt
      901 tttgtggagt tggtgaatgc ccttggtcaa tataatgatg atgacgatga tgatgatgga
      961 gacgatcctg aagaaagaga agaaaagcag aaagatctgg aggatcaccg agatgataaa
     1021 gaaagccgcc cacctcggaa atttccttct gataaaattt ttgaagccat ttcctcaatg
     1081 tttccagata agggcacagc agaagaacta aaggaaaaat ataaagaact caccgaacag
     1141 cagctcccag gcgcacttcc tcctgaatgt acccccaaca tagatggacc aaatgctaaa
     1201 tctgttcaga gagagcaaag cttacactcc tttcatacgc ttttctgtag gcgatgtttt
     1261 aaatatgact gcttcctaca tcgtaagtgc aattattctt ttcatgcaac acccaacact
     1321 tataagcgga agaacacaga aacagctcta gacaacaaac cttgtggacc acagtgttac
     1381 cagcatttgg agggagcaaa ggagtttgct gctgctctca ccgctgagcg gataaagacc
     1441 ccaccaaaac gtccaggagg ccgcagaaga ggacggcttc ccaataacag tagcaggccc
     1501 agcaccccca ccattaatgt gctggaatca aaggatacag acagtgatag ggaagcaggg
     1561 actgaaacgg ggggagagaa caatgataaa gaagaagaag agaagaaaga tgaaacttcg
     1621 agctcctctg aagcaaattc tcggtgtcaa acaccaataa agatgaagcc aaatattgaa
     1681 cctcctgaga atgtggagtg gagtggtgct gaagcctcaa tgtttagagt cctcattggc
     1741 acttactatg acaatttctg tgccattgct aggttaattg ggaccaaaac atgtagacag
     1801 gtgtatgagt ttagagtcaa agaatctagc atcatagctc cagctcccgc tgaggatgtg
     1861 gatactcctc caaggaaaaa gaagaggaaa caccggttgt gggctgcaca ctgcagaaag
     1921 atacagctga aaaaggacgg ctcctctaac catgtttaca actatcaacc ctgtgatcat
     1981 ccacggcagc cttgtgacag ttcgtgccct tgtgtgatag cacaaaattt ttgtgaaaag
     2041 ttttgtcaat gtagttcaga gtgtcaaaac cgctttccgg gatgccgctg caaagcacag
     2101 tgcaacacca agcagtgccc gtgctacctg gctgtccgag agtgtgaccc tgacctctgt
     2161 cttacttgtg gagccgctga ccattgggac agtaaaaatg tgtcctgcaa gaactgcagt
     2221 attcagcggg gctccaaaaa gcatctattg ctggcaccat ctgacgtggc aggctggggg
     2281 atttttatca aagatcctgt gcagaaaaat gaattcatct cagaatactg tggagagatt
     2341 atttctcaag atgaagctga cagaagaggg aaagtgtatg ataaatacat gtgcagcttt
     2401 ctgttcaact tgaacaatga ttttgtggtg gatgcaaccc gcaagggtaa caaaattcgt
     2461 tttgcaaatc attcggtaaa tccaaactgc tatgcaaaag ttatgatggt taacggtgat
     2521 cacaggatag gtatttttgc caagagagcc atccagactg gcgaagagct gttttttgat
     2581 tacagataca gccaggctga tgccctgaag tatgtcggca tcgaaagaga aatggaaatc
     2641 ccttgcccaa ctttcttgta caaagtggta tacccatacg atgttccaga ttacgcttag
     2701 taatgagaaa tactcagaag atgtcactca ccatagtccg cagtgaaaga taaatgatcg
     2761 gcaacgcact tgacgccagg ttttagagct agaaatagca agtaggccac tgagcaagaa
     2821 ctatgaatga gatctttaga catattgcgg ccgctttctc cgcgatccag acatgataag
     2881 atacattgat gagtttggac aaaccaaaac tagaatgcag tgaaaaaaat gccttatttg
     2941 tgaaatttgt gatgctattg ccttatttgt aaccattata agctgcaata aacaagttat
     3001 ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat
     3061 ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg
     3121 actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc
     3181 acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt
     3241 tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct
     3301 agggttaaga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     3481 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     3541 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     3601 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccatgtct
     3661 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacacgcg agttcgaaag
     3721 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3781 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3841 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3901 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3961 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     4021 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     4081 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     4141 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     4201 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     4261 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     4321 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     4381 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     4441 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     4501 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     4561 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     4621 gagcgtggtt cacgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4681 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4741 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4801 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4861 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     5101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     5401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     5461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     5521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     5581 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     5641 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5701 gaggaagcgg atgagcgccc aatacgcaaa ccgcctctca ccgcgcgttg gccgattcat
     5761 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaaac
     5821 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     5881 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     5941 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6001 ttcacacagg aaacagctat gaccatgatt acgccaaggt cgacttaacc ctagaaagat
     6061 aatcatattg tgacgtacgt taaagataat catgcgtaaa attgacgcat gtgttttatc
     6121 ggtctgtata tcgaggttta tttattaatt tgaatagata ttaagtttta ttatatttac
     6181 acttacatac taataataaa ttcaacaaac aatttattta tgtttattta tttattaaaa
     6241 aaaaacaaaa actcaaaatt tcttctataa agtaacaaaa cttttagcag tgaaaaaaat
     6301 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     6361 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     6421 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctgg
     6481 agattcatgc gccaggtttt ctggtcatgc accacgttcg tgggccctca ggaacctcca
     6541 cgtcagcggt aactgtgaag cccagtcgct cgtaaaaagg aagattccgg ggagcactgg
     6601 tttcgaggaa cgccggcacg ccagcccgtt ccgcggcctc aacaccagga agaacgacag
     6661 cgcttcccaa acctttacct tggtgatctg ggctgacacc gacagtggca agaaaccacg
     6721 caggctcctt tggtctatga ggagccagca agccctccat ttgctgttgt gccgccaacc
     6781 tactccctga cagctcggcc atccgcggac ctatttccgc gaacactgcg ccggcttcga
     6841 cgctttccgg agtagtccag accgcaactg cggcaccatc gtccgcaacc caaactttac
     6901 caatgtccag acccacgcgc gtaagaaaca gctcttgcaa ctcagttacc ctctctatgt
     6961 gtctgtccgg atctacggta tggcgggtcg ctggataatc tgcaaatgct gcggcaagtg
     7021 ttcggacagc ccgtggtaca tcgtcacggg tagcgagcct aacagtgggc ttatattccg
     7081 tcatggggcc ggggttctcc tccacgtcgc cggcctgctt cagcaggctg aagttggtgg
     7141 cgccgctgcc cccggggagc atgtcaaggt caaaatcgtc aagagcgtca gcaggcagca
     7201 tatcaaggtc aaagtcgtca agggcatcgg ctgggagcat gtctaagtca aaatcgtcaa
     7261 gggcgtcggt cggcccgccg ctttcgcact ttagctgttt ctccaggcca catatgatta
     7321 gttccaggcc gaaaaggaag gcaggttcgg ctccctgccg gtcgaacagc tcaattgctt
     7381 gtttcagaag tgggggcata gaatcggtgg taggtgtctc tctttcctct tttgctactt
     7441 gatgctcctg ttcctccaat acgcagccca gtgtaaagtg gcccacggcg gacagagcgt
     7501 acagtgcgtt ctccagggag aagccttgct gacacaggaa cgcgagctga ttttccaggg
     7561 tttcgtactg tttctctgtt gggcgggtgc cgagatgcac tttagccccg tcacgatgtg
     7621 agaggagagc acagcggtat gacttggcgt tgttccgcag aaagtcttgc catgactcgc
     7681 cttccagggg gcaggagtgg gtatgatgcc tgtccagcat ctcgattggc agggcatcga
     7741 gcagggcccg cttgttcttc acgtgccagt acagggtagg ctgctcaact cccagctttt
     7801 gagcgagttt ccttgtcgtc aggccttcga taccgactcc attgagtaat tccagagcag
     7861 agtttatgac tttgctcttg tccagtctag acatcttatc gtcatcgtct ttgtaatcca
     7921 tggtggcgga tcccgcgtca cgacacctgt gttctggcgg caaacccgtt gcgaaaaaga
     7981 acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg aaaaaggcgg
     8041 agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta ggcaccggtt
     8101 caattgccga cccctccccc caacttctcg ggcactgtgg gccatgtgcg ctctgcccca
     8161 tggtaatagc catgactaat acgtagatgt actgccaagt aggaaagtgc cataaggtca
     8221 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     8281 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     8341 attgacgtca atggaaagtg cctattggcg ttactatggg aacatacgtc attattgacg
     8401 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     8461 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     8521 tagtcaataa tcaatgtcat aagctctaat cgcataatcg agctaagagc gattaatatg
     8581 cttagctagc ttagctaagc tagatttctc tattctccca caatatatag cagctattag
     8641 cttagcagtc cgaatacgat cgtccacgat ccataagcgt aacacatttg agtagcgact
     8701 gacgggcacc ggagccactc gagtggaatt a
//
