LOCUS       sequence-439990-       17181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17181)
  AUTHORS   Muzumdar MD, Tasic B, Miyamichi K, Li L, Luo L
  TITLE     A global double-fluorescent Cre reporter mouse.
  JOURNAL   Genesis. 2007 Sep . 45(9):593-605.
  PUBMED    17868096
REFERENCE   2  (bases 1 to 17181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2007 Sep . 45(9):593-605."
COMMENT     Sequence Label: Rosa26 mT/mG
FEATURES             Location/Qualifiers
     source          1..17181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3..1087
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     enhancer        1183..1486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1488..1767
                     /label=chicken beta-actin promoter
     intron          1768..2784
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2708..2730
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2792..2811
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(2886..2919)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2962..4392
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     intron          4483..4548
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4678..4698
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5123..5257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5160..5179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5214..5233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(5299..5332)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(5489..5491,5492..6205)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5489..5491,5492..6202)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5531..5552)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5792..5811)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6139..6160
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6226..6450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6478..6511
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        6575..7074
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     promoter        7086..7133
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7152..7955
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7215..7234)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7825..7844
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7993..8217
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    8272..8305
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    8342..12683
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        12733..13232
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             13259..13831
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          13908..13973
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    14047..14271
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(14333..14353)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14333..14351)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14372..14388)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14372..14388)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14385..14407)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14396..14412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14420..14426,14427..14444,14445..14450))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14465..14486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14603..14620)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14774..15362)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14854..14873)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15533..16324,16325..16393))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16156..16175
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16394..16498)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      16525..16980
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16612..16631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16822..16843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     17106..17128
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     17120..17137
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     17121..17137
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17147..17166
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        17147..17165
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
       61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
      121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
      181 ggggggcagc ggaggagcgc ttccggccga cgtctcgtcg ctgattggct tcttttcctc
      241 ccgccgtgtg tgaaaacaca aatggcgtgt tttggttggc gtaaggcgcc tgtcagttaa
      301 cggcagccgg agtgcgcagc cgccggcagc ctcgctctgc ccactgggtg gggcgggagg
      361 taggtggggt gaggcgagct ggacgtgcgg gcgcggtcgg cctctggcgg ggcgggggag
      421 gggagggagg gtcagcgaaa gtagctcgcg cgcgagcggc cgcccaccct ccccttcctc
      481 tgggggagtc gttttacccg ccgccggccg ggcctcgtcg tctgattggc tctcggggcc
      541 cagaaaactg gcccttgcca ttggctcgtg ttcgtgcaag ttgagtccat ccgccggcca
      601 gcgggggcgg cgaggaggcg ctcccaggtt ccggccctcc cctcggcccc gcgccgcaga
      661 gtctggccgc gcgcccctgc gcaacgtggc aggaagcgcg cgctgggggc ggggacgggc
      721 agtagggctg agcggctgcg gggcgggtgc aagcacgttt ccgacttgag ttgcctcaag
      781 aggggcgtgc tgagccagac ctccatcgcg cactccgggg agtggaggga aggagcgagg
      841 gctcagttgg gctgttttgg aggcaggaag cacttgctct cccaaagtcg ctctgagttg
      901 ttatcagtaa gggagctgca gtggagtagg cggggagaag gccgcaccct tctccggagg
      961 ggggagggga gtgttgcaat acctttctgg gagttctctg ctgcctcctg gcttctgagg
     1021 accgccctgg gcctgggaga atcccttccc cctcttccct cgtgatctgc aactccagtc
     1081 tttctagcct taattaaccg tttaaacaat tctgcaggaa tctagttatt aatagtaatc
     1141 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1201 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1261 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1321 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     1381 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     1441 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     1501 cacgttctgc ttcactctcc ccatctcccc ccccctcccc acccccaatt ttgtatttat
     1561 ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg gcgcgcgcca
     1621 ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc
     1681 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
     1741 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
     1801 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
     1861 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
     1921 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
     1981 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
     2041 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
     2101 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
     2161 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
     2221 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
     2281 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
     2341 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
     2401 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
     2461 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
     2521 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
     2581 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
     2641 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     2701 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     2761 catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat
     2821 cattttggca aagaattgat ttgataccgc gggctcgaca ctagtgaacc tcttcgaggg
     2881 acctaataac ttcgtatagc atacattata cgaagttata ttaagggttc cggatccatg
     2941 ggttgctgtt tctccaagac catggtgagc aagggagagg aggtcatcaa agagttcatg
     3001 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     3061 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     3121 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     3181 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     3241 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     3301 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     3361 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     3421 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     3481 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     3541 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     3601 cagtacgagc gctccgaggg ccgccaccac ctgttcctgg ggcatggcac cggcagcacc
     3661 ggcagcggca gctccggcac cgcctcctcc gaggacaaca acatggccgt catcaaagag
     3721 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
     3781 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     3841 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
     3901 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
     3961 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
     4021 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
     4081 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
     4141 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
     4201 ggccgctacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
     4261 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
     4321 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag
     4381 ctgtacaagt aactcgagat tcgagggatc tttgtgaagg aaccttactt ctgtggtgtg
     4441 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     4501 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     4561 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
     4621 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
     4681 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
     4741 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
     4801 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
     4861 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
     4921 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
     4981 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
     5041 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     5101 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     5161 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5221 gtccaaactc atcaatgtat cttatcatgt ctggatctga catggtaagt aagcttgggc
     5281 tgcaggtcga gggacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg
     5341 ttccggatca tcaccgcgga tgggttgctg tttctccaag accgcagcga agggagaagc
     5401 cgccgcggag aggcctgggg aggcggctgt ggcctcgtcg ccttccaaag cgaacggaca
     5461 ggagaatggc cacgtgaagg taggatccgt gagcaagggc gaggagctgt tcaccggggt
     5521 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     5581 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     5641 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     5701 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     5761 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     5821 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     5881 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     5941 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     6001 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     6061 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     6121 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     6181 cggcatggac gagctgtaca agtaaagcgg ccgctcgagc ctcgactgtg ccttctagtt
     6241 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6301 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6361 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6421 ggcatgctgg ggatgcggtg ggctctatgg cttctgagca taggatcccc gaattccgaa
     6481 gttcctattc tctagaaagt ataggaactt caggtctgaa gaggagttta cgtccagcca
     6541 agctagcttg gctgcaggtc gtcgaaattc taccgggtag gggaggcgct tttcccaagg
     6601 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     6661 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6721 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6781 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6841 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6901 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     6961 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     7021 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     7081 agcctgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     7141 ggaactaaac catgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
     7201 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     7261 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     7321 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     7381 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     7441 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     7501 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     7561 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     7621 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     7681 tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc
     7741 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     7801 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     7861 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     7921 tcgccttcta tcgccttctt gacgagttct tctgagggga tcaattctct agagctcgct
     7981 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     8041 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     8101 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     8161 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     8221 ctgaggcgga aagaaccagc tggggctcga ctagagcttg cggaaccctt cgaagttcct
     8281 attctctaga aagtatagga acttcatcag tcaggtacat aatataactt cggcgcgccg
     8341 gctagaagat gggcgggagt cttctgggca ggcttaaagg ctaacctggt gtgtgggcgt
     8401 tgtcctgcag gggaattgaa caggtgtaaa attggaggga caagacttcc cacagatttt
     8461 cggttttgtc gggaagtttt ttaatagggg caaataagga aaatgggagg ataggtagtc
     8521 atctggggtt ttatgcagca aaactacagg ttattattgc ttgtgatccg cctcggagta
     8581 ttttccatcg aggtagatta aagacatgct cacccgagtt ttatactctc ctgcttgaga
     8641 tccttactac agtatgaaat tacagtgtcg cgagttagac tatgtaagca gaattttaat
     8701 catttttaaa gagcccagta cttcatatcc atttctcccg ctccttctgc agccttatca
     8761 aaaggtattt tagaacactc attttagccc cattttcatt tattatactg gcttatccaa
     8821 cccctagaca gagcattggc attttccctt tcctgatctt agaagtctga tgactcatga
     8881 aaccagacag attagttaca tacaccacaa atcgaggctg tagctggggc ctcaacactg
     8941 cagttctttt ataactcctt agtacacttt ttgttgatcc tttgccttga tccttaattt
     9001 tcagtgtcta tcacctctcc cgtcaggtgg tgttccacat ttgggcctat tctcagtcca
     9061 gggagtttta caacaataga tgtattgaga atccaaccta aagcttaact ttccactccc
     9121 atgaatgcct ctctcctttt tctccattta taaactgagc tattaaccat taatggtttc
     9181 caggtggatg tctcctcccc caatattacc tgatgtatct tacatattgc caggctgata
     9241 ttttaagaca ttaaaaggta tatttcatta ttgagccaca tggtattgat tactgcttac
     9301 taaaattttg tcattgtaca catctgtaaa aggtggttcc ttttggaatg caaagttcag
     9361 gtgtttgttg tctttcctga cctaaggtct tgtgagcttg tattttttct atttaagcag
     9421 tgctttctct tggactggct tgactcatgg cattctacac gttattgctg gtctaaatgt
     9481 gattttgcca agcttcttca ggacctataa ttttgcttga cttgtagcca aacacaagta
     9541 aaatgattaa gcaacaaatg tatttgtgaa gcttggtttt taggttgttg tgttgtgtgt
     9601 gcttgtgctc tataataata ctatccaggg gctggagagg tggctcggag ttcaagagca
     9661 cagactgctc ttccagaagt cctgagttca attcccagca accacatggt ggctcacaac
     9721 catctgtaat gggatctgat gccctcttct ggtgtgtctg aagaccacaa gtgtattcac
     9781 attaaataaa taaatcctcc ttcttcttct tttttttttt tttaaagaga atactgtctc
     9841 cagtagaatt tactgaagta atgaaatact ttgtgtttgt tccaatatgg tagccaataa
     9901 tcaaattact ctttaagcac tggaaatgtt accaaggaac taatttttat ttgaagtgta
     9961 actgtggaca gaggagccat aactgcagac ttgtgggata cagaagacca atgcagactt
    10021 taatgtcttt tctcttacac taagcaataa agaaataaaa attgaacttc tagtatccta
    10081 tttgtttaaa ctgctagctt tacttaactt ttgtgcttca tctatacaaa gctgaaagct
    10141 aagtctgcag ccattactaa acatgaaagc aagtaatgat aattttggat ttcaaaaatg
    10201 tagggccaga gtttagccag ccagtggtgg tgcttgcctt tatgccttta atcccagcac
    10261 tctggaggca gagacaggca gatctctgag tttgagccca gcctggtcta cacatcaagt
    10321 tctatctagg atagccagga atacacacag aaaccctgtt ggggaggggg gctctgagat
    10381 ttcataaaat tataattgaa gcattcccta atgagccact atggatgtgg ctaaatccgt
    10441 ctacctttct gatgagattt gggtattatt ttttctgtct ctgctgttgg ttgggtcttt
    10501 tgacactgtg ggctttcttt aaagcctcct tcctgccatg tggtctcttg tttgctacta
    10561 acttcccatg gcttaaatgg catggctttt tgccttctaa gggcagctgc tgagatttgc
    10621 agcctgattt ccagggtggg gttgggaaat ctttcaaaca ctaaaattgt cctttaattt
    10681 tttttttaaa aaatgggtta tataataaac ctcataaaat agttatgagg agtgaggtgg
    10741 actaatatta aatgagtccc tcccctataa aagagctatt aaggcttttt gtcttatact
    10801 taactttttt tttaaatgtg gtatctttag aaccaagggt cttagagttt tagtatacag
    10861 aaactgttgc atcgcttaat cagattttct agtttcaaat ccagagaatc caaattcttc
    10921 acagccaaag tcaaattaag aatttctgac ttttaatgtt aatttgctta ctgtgaatat
    10981 aaaaatgata gcttttcctg aggcagggtc tcactatgta tctctgcctg atctgcaaca
    11041 agatatgtag actaaagttc tgcctgcttt tgtctcctga atactaaggt taaaatgtag
    11101 taatactttt ggaacttgca ggtcagattc ttttataggg gacacactaa gggagcttgg
    11161 gtgatagttg gtaaaatgtg tttcaagtga tgaaaacttg aattattatc accgcaacct
    11221 actttttaaa aaaaaaagcc aggcctgtta gagcatgctt aagggatccc taggacttgc
    11281 tgagcacaca agagtagtta cttggcaggc tcctggtgag agcatatttc aaaaaacaag
    11341 gcagacaacc aagaaactac agttaaggtt acctgtcttt aaaccatctg catatacaca
    11401 gggatattaa aatattccaa ataatatttc attcaagttt tcccccatca aattgggaca
    11461 tggatttctc cggtgaatag gcagagttgg aaactaaaca aatgttggtt ttgtgatttg
    11521 tgaaattgtt ttcaagtgat agttaaagcc catgagatac agaacaaagc tgctatttcg
    11581 aggtctcttg gtttatactc agaagcactt ctttgggttt ccctgcacta tcctgatcat
    11641 gtgctaggcc taccttaggc tgattgttgt tcaaataaac ttaagtttcc tgtcaggtga
    11701 tgtcatatga tttcatatat caaggcaaaa catgttatat atgttaaaca tttgtactta
    11761 atgtgaaagt taggtctttg tgggtttgat ttttaatttt caaaacctga gctaaataag
    11821 tcatttttac atgtcttaca tttggtggaa ttgtataatt gtggtttgca ggcaagactc
    11881 tctgacctag taaccctacc tatagagcac tttgctgggt cacaagtcta ggagtcaagc
    11941 atttcacctt gaagttgaga cgttttgtta gtgtatacta gtttatatgt tggaggacat
    12001 gtttatccag aagatattca ggactatttt tgactgggct aaggaattga ttctgattag
    12061 cactgttagt gagcattgag tggcctttag gcttgaattg gagtcacttg tatatctcaa
    12121 ataatgctgg ccttttttaa aaagcccttg ttctttatca ccctgttttc tacataattt
    12181 ttgttcaaag aaatacttgt ttggatctcc ttttgacaac aatagcatgt tttcaagcca
    12241 tatttttttt cctttttttt tttttttttt ggtttttcga gacagggttt ctctgtatag
    12301 ccctggctgt cctggaactc actttgtaga ccaggctggc ctcgaactca gaaatccgcc
    12361 tgcctctgcc tcctgagtgc cgggattaaa ggcgtgcacc accacgcctg gctaagttgg
    12421 atattttgtt atataactat aaccaatact aactccactg ggtggatttt taattcagtc
    12481 agtagtctta agtggtcttt attggccctt cattaaaatc tactgttcac tctaacagag
    12541 gctgttggta ctagtggcac ttaagcaact tcctacggat atactagcag attaagggtc
    12601 agggatagaa actagtctag cgttttgtat acctaccagc tttatactac cttgttctga
    12661 tagaaatatt tcaggacatc tagcttatcg ataccgtcga cggtatcgat aagcttgata
    12721 tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
    12781 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
    12841 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
    12901 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
    12961 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
    13021 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
    13081 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
    13141 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
    13201 tgttctcctc ttcctcatct ccgggccttt cgacctgcag gtcctcgcca tggatcctga
    13261 tgatgttgtt gattcttcta aatcttttgt gatggaaaac ttttcttcgt accacgggac
    13321 taaacctggt tatgtagatt ccattcaaaa aggtatacaa aagccaaaat ctggtacaca
    13381 aggaaattat gacgatgatt ggaaagggtt ttatagtacc gacaataaat acgacgctgc
    13441 gggatactct gtagataatg aaaacccgct ctctggaaaa gctggaggcg tggtcaaagt
    13501 gacgtatcca ggactgacga aggttctcgc actaaaagtg gataatgccg aaactattaa
    13561 gaaagagtta ggtttaagtc tcactgaacc gttgatggag caagtcggaa cggaagagtt
    13621 tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc agccttccct tcgctgaggg
    13681 gagttctagc gttgaatata ttaataactg ggaacaggcg aaagcgttaa gcgtagaact
    13741 tgagattaat tttgaaaccc gtggaaaacg tggccaagat gcgatgtatg agtatatggc
    13801 tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt gaaggaacct tacttctgtg
    13861 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
    13921 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
    13981 cctatggaac tgatgaatgg gagcagtggt ggaatgcaga tcctagagct cgctgatcag
    14041 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
    14101 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
    14161 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    14221 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
    14281 cggaaagaac cagctggggc tcgagggggg gcccggtacc cagcttttgt tccctttagt
    14341 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    14401 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    14461 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    14521 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    14581 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    14641 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    14701 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    14761 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    14821 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    14881 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    14941 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    15001 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    15061 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    15121 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    15181 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    15241 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    15301 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    15361 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    15421 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    15481 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    15541 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    15601 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    15661 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    15721 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    15781 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    15841 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    15901 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    15961 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    16021 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    16081 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    16141 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    16201 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    16261 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    16321 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    16381 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    16441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    16501 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
    16561 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
    16621 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
    16681 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
    16741 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
    16801 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
    16861 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
    16921 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
    16981 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    17041 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    17101 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
    17161 atagggcgaa ttggagctcc a
//
