LOCUS       sequence-440003-        9568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  control shRNA plasmid with EGFP expression in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Libe-Philippot B, Iwata R, Recupero AJ, Wierda K, Bernal Garcia S, 
            Hammond L, van Benthem A, Limame R, Ditkowska M, Beckers S, 
            Gaspariunaite V, Peze-Heidsieck E, Remans D, Charrier C, Theys T, 
            Polleux F, Vanderhaeghen P
  TITLE     Synaptic neoteny of human cortical neurons requires species-specific
            balancing of SRGAP2-SYNGAP1 cross-inhibition.
  JOURNAL   Neuron. 2024 Oct 9:S0896-6273(24)00645-7. doi: 
            10.1016/j.neuron.2024.08.021.
  PUBMED    39406239
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2024 Oct 9:S0896-6273(24)00645-7. doi: 
            10.1016/j.neuron.2024.08.021."
COMMENT     Sequence Label: pLV-H1-shScramble-hsynapsin-EGFP
FEATURES             Location/Qualifiers
     source          1..9568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             510..690
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(716..733)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    722..946
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      992..1420
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1079..1098)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1289..1310
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1429..1449)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1434..1763
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1614..1749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1676..1695
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1811..1858
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1877..2251
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2381..2502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2418..2437)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2472..2491
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2551..2567)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2575..2591
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2599..2605,2606..2623,2624..2629))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2644..2665
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2782..2799)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2953..3541)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3033..3052)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3712..4503,4504..4572))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4335..4354
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4573..4677)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4752..4771)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4943..5322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5324..5522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5477..5497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5540..5720
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5767..5892
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6385..6618
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6803..6847
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7145..7262
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(7334..7339,7340..7357,7358..7364)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7372..7388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7396..7412
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7499..7713)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(7559..7578)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        7740..8187
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      8202..8211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8208..8210,8211..8213,8214..8927)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8253..8274)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8514..8533)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8861..8882
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8961..9549
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9014..9034)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9432..9443)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9552..9568)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
       61 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
      121 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
      181 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
      241 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
      301 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
      361 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
      421 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
      481 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
      541 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      601 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      661 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg
      721 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
      781 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
      841 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
      901 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
      961 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     1021 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     1081 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     1141 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     1201 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     1261 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1321 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     1381 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     1441 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     1501 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     1561 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     1621 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     1681 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     1741 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     1801 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     1861 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     1921 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     1981 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga
     2041 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta
     2101 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac
     2161 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg
     2221 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     2281 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     2341 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     2401 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2461 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     2521 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2581 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2641 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2701 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2761 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2821 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2881 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2941 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3001 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3061 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3121 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3181 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3241 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3301 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3361 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3421 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3481 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3541 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3601 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3661 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3721 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3781 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3841 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3901 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3961 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4021 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4081 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4141 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4201 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4261 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4321 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4381 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4441 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4501 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4561 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4621 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4681 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     4741 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     4801 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     4861 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     4921 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     4981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     5041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     5101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     5161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     5221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     5281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     5341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     5401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     5461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     5521 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5581 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5641 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5701 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     5761 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     5821 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     5881 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     5941 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     6001 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     6061 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     6121 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     6181 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     6241 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     6301 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     6361 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     6421 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     6481 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     6541 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     6601 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     6661 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     6721 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     6781 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     6841 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     6901 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     6961 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     7021 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     7081 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     7141 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     7201 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     7261 ttcgggttta ttacagggac agcagagatc cagtttggtt aatgagttag ctcactcatt
     7321 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7381 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctg ctagcaaaaa
     7441 aacacctata acaacggtag gtgacaggaa gcctaccgtt gttataggtg tgggatccgt
     7501 ggtctcatac agaacttata agattcccaa atccaaagac atttcacgtt tatggtgatt
     7561 tcccagaaca catagcgaca tgcaaatatt gcagggcgcc actcccctgt ccctcacagc
     7621 catcttcctg ccagggcgca cgcgcgctgg gtgttcccgc ctagtgacac tgggcccgcg
     7681 attccttgga gcgggttgat gacgtcagcg ttcgaattct gcagagggcc ctgcgtatga
     7741 gtgcaagtgg gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac
     7801 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
     7861 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
     7921 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
     7981 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
     8041 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
     8101 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
     8161 ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggt cgccaccatg gtgagcaagg
     8221 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     8281 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     8341 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     8401 tgacctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     8461 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     8521 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     8581 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     8641 actacaacag ccacaaggtc tatatcaccg ccgacaagca gaagaacggc atcaaggtga
     8701 acttcaagac ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     8761 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     8821 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     8881 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagta caagtaaaat
     8941 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     9001 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     9061 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     9121 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     9181 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     9241 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     9301 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     9361 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     9421 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     9481 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     9541 cctccccgca tcgataccgt cgacctcg
//
