LOCUS       sequence-440015-       12431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ezrin activation reporter, T567 phosphorylation deficient.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Marshall-Burghardt S, Migueles-Ramirez RA, Lin Q, El Baba N, Saada 
            R, Umar M, Mavalwala K, Hayer A
  TITLE     Excitable Rho dynamics control cell shape and motility by 
            sequentially activating ERM proteins and actomyosin contractility.
  JOURNAL   Sci Adv. 2024 Sep 6;10(36):eadn6858. doi: 10.1126/sciadv.adn6858. 
            Epub 2024 Sep 6.
  PUBMED    39241071
REFERENCE   2  (bases 1 to 12431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-09-6"; volume: "10(36):eadn6858. doi"; pages: " 
            10.1126/sciadv.adn6858. Epub 2024 Sep 6"
COMMENT     Sequence Label: pLV-miRFP680-NES-p2A-ezrin-T567A-mRuby3-IRES-Neo
FEATURES             Location/Qualifiers
     source          1..12431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2220..2431
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2432..3370
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3327..3347
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4379..4435
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6215..6925
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     misc_feature    7029..7578
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7173..7190)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7400..7419
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7581..8375
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7635..7654)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8245..8264
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    8399..8987
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8452..8472)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8870..8881)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9194..9827
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9955..9971)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9955..9971)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9968..9990)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9979..9995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10003..10009,10010..10027,10028..10033))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10048..10069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10186..10203)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10357..10945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10437..10456)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11116..11907,11908..11976))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11739..11758
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11977..12081)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12129..12263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12166..12185)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12220..12239
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(12431,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgaggtg cccgtcagtg
     2221 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2281 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2341 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2401 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2461 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     2521 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     2581 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     2641 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     2701 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     2761 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     2821 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     2881 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     2941 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3001 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3061 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3121 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3181 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3241 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3301 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3361 tccatttcag gtgtcgtgag gatccatggc cgaaggatct gtggccagac agcctgatct
     3421 gctgacctgc gacgacgagc ccattcatat ccctggcgcc attcagcctc acggactgct
     3481 gcttgctctg gccgccgata tgacaatcgt ggccggcagc gataacctgc ctgagctgac
     3541 aggactggcc atcggagccc tgattggaag atctgccgcc gacgtgttcg acagcgagac
     3601 acacaacaga ctgacaatcg ccctggccga acctggtgct gctgtgggag cacctatcac
     3661 cgtgggcttc accatgagaa aggacgccgg ctttatcggc agctggcaca gacacgacca
     3721 gctgatcttt ctggaactgg aacctcctca gagagatgtg gccgagcctc aggccttctt
     3781 cagaaggacc aacagcgcca tcagacggct gcaggccgct gagacactgg aatctgcttg
     3841 tgccgccgct gctcaagaag tgcggaagat caccggcttc gaccgcgtga tgatctacag
     3901 attcgccagc gacttcagcg gcgaagtgat cgccgaagat agatgcgccg aggtggaaag
     3961 caagctgggc ctgcattacc ctgccagcac agttcctgct caggccagaa ggctgtacac
     4021 catcaatccc gtgcggatca tccccgacat caactacaga cccgtgcctg tgacacccga
     4081 tctgaaccct gttaccggca gacccatcga cctgagcttc gccatcctga gaagcgtgtc
     4141 cccatgccac ctggaattca tgcggaacat cggcatgcac ggcaccatga gcatctctat
     4201 cctgcggggc gaaagactgt ggggcctgat cgtgtgtcac cacagaaccc cttactacgt
     4261 ggacctggat ggcagacagg cctgcaaaag agtggccgag agactggcca cacagatcgg
     4321 cgtgatggaa gagtctggac tggccctgaa actggccggc ctggatattg gatccggagc
     4381 cacgaacttc tctctgttaa agcaagcagg agacgtggaa gaaaatcccg gtcctgcggc
     4441 cgccatgccg aaaccaatca atgtccgagt taccaccatg gatgcagagc tggagtttgc
     4501 aatccagcca aatacaactg gaaaacagct ttttgatcag gtggtaaaga ctatcggcct
     4561 ccgggaagtg tggtactttg gcctccacta tgtggataat aaaggatttc ctacctggct
     4621 gaagctggat aagaaggtgt ctgcccagga ggtcaggaag gagaatcccc tccagttcaa
     4681 gttccgggcc aagttctacc ctgaagatgt ggctgaggag ctcatccagg acatcaccca
     4741 gaaacttttc ttcctccaag tgaaggaagg aatccttagc gatgagatct actgcccccc
     4801 tgagactgcc gtgctcttgg ggtcctacgc tgtgcaggcc aagtttgggg actacaacaa
     4861 agaagtgcac aagtctgggt acctcagctc tgagcggctg atccctcaaa gagtgatgga
     4921 ccagcacaaa cttaccaggg accagtggga ggaccggatc caggtgtggc atgcggaaca
     4981 ccgtgggatg ctcaaagata atgctatgtt ggaatacctg aagattgctc aggacctgga
     5041 aatgtatgga atcaactatt tcgagataaa aaacaagaaa ggaacagacc tttggcttgg
     5101 agttgatgcc cttggactga atatttatga gaaagatgat aagttaaccc caaagattgg
     5161 ctttccttgg agtgaaatca ggaacatctc tttcaatgac aaaaagtttg tcattaaacc
     5221 catcgacaag aaggcacctg actttgtgtt ttatgcccca cgtctgagaa tcaacaagcg
     5281 gatcctgcag ctctgcatgg gcaaccatga gttgtatatg cgccgcagga agcctgacac
     5341 catcgaggtg cagcagatga aggcccaggc ccgggaggag aagcatcaga agcagctgga
     5401 gcggcaacag ctggaaacag agaagaaaag gagagaaacc gtggagagag agaaagagca
     5461 gatgatgcgc gagaaggagg agttgatgct gcggctgcag gactatgagg agaagacaaa
     5521 gaaggcagag agagagctct cggagcagat tcagagggcc ctgcagctgg aggaggagag
     5581 gaagcgggca caggaggagg ccgagcgcct agaggctgac cgtatggctg cactgcgggc
     5641 taaggaggag ctggagagac aggcggtgga tcagataaag agccaggagc agctggctgc
     5701 ggagcttgca gaatacactg ccaagattgc cctcctggaa gaggcgcgga ggcgcaagga
     5761 ggatgaagtt gaagagtggc agcacagggc caaagaagcc caggatgacc tggtgaagac
     5821 caaggaggag ctgcacctgg tgatgacagc acccccgccc ccaccacccc ccgtgtacga
     5881 gccggtgagc taccatgtcc aggagagctt gcaggatgag ggcgcagagc ccacgggcta
     5941 cagcgcggag ctgtctagtg agggcatccg ggatgaccgc aatgaggaga agcgcatcac
     6001 tgaggcagag aagaacgagc gtgtgcagcg gcagctgctg acgctgagca gcgagctgtc
     6061 ccaggcccga gatgagaata agaggaccca caatgacatc atccacaacg agaacatgag
     6121 gcaaggccgg gacaagtaca aggcactgcg gcagatccgg cagggcaaca ccaagcagcg
     6181 catcgacgag ttcgaggccc tgagtgctgg tggtatggtg tctaagggcg aagagctgat
     6241 caaggaaaat atgcgtatga aggtggtcat ggaaggttcg gtcaacggcc accaattcaa
     6301 atgcacaggt gaaggagaag gcagaccgta cgagggagtg caaaccatga ggatcaaagt
     6361 catcgaggga ggacccctgc catttgcctt tgacattctt gccacgtcgt tcatgtatgg
     6421 cagccgtacc tttatcaagt acccggccga catccctgat ttctttaaac agtcctttcc
     6481 tgagggtttt acttgggaaa gagttacgag atacgaagat ggtggagtcg tcaccgtcac
     6541 gcaggacacc agccttgagg atggcgagct cgtctacaac gtcaaggtca gaggggtaaa
     6601 ctttccctcc aatggtcccg tgatgcagaa gaagaccaag ggttgggagc ctaatacaga
     6661 gatgatgtat ccagcagatg gtggtctgag aggatacact gacatcgcac tgaaagttga
     6721 tggtggtggc catctgcact gcaacttcgt gacaacttac aggtcaaaaa agaccgtcgg
     6781 gaacatcaag atgcccggtg tccatgccgt tgatcaccgc ctggaaagga tcgaggagag
     6841 tgacaatgaa acctacgtag tgcaaagaga agtggcagtt gccaaataca gcaaccttgg
     6901 tggtggcatg gacgagctgt acaagtaagc ggccgctcta gagtcgacgg gccgcggtaa
     6961 caattgttaa ctaacttaag ctagcaacgg tttccctcta gcgggatcaa ttccgccccc
     7021 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     7081 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     7141 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     7201 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     7261 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     7321 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     7381 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     7441 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     7501 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     7561 ttgaaaaaca cgataatacc atgattgaac aagatggatt gcacgcaggt tctccggccg
     7621 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     7681 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     7741 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     7801 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     7861 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     7921 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     7981 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     8041 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     8101 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     8161 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     8221 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     8281 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     8341 tcgccttcta tcgccttctt gacgagttct tctgacccgg gcggggcgcg tctggaacaa
     8401 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     8461 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     8521 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     8581 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     8641 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     8701 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     8761 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     8821 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     8881 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     8941 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     9001 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     9061 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     9121 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     9181 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     9241 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     9301 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     9361 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     9421 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     9481 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     9541 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     9601 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     9661 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9721 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9781 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     9841 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     9901 ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     9961 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    10021 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    10081 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    10141 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    10201 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    10261 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
    10321 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
    10381 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
    10441 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
    10501 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
    10561 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
    10621 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
    10681 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
    10741 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
    10801 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
    10861 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
    10921 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
    10981 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11041 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
    11101 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
    11161 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
    11221 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
    11281 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
    11341 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
    11401 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
    11461 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
    11521 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    11581 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    11641 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    11701 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    11761 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    11821 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    11881 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    11941 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    12001 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    12061 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
    12121 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    12181 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    12241 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    12301 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    12361 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    12421 acttagtagt t
//
