LOCUS       sequence-440033-       17843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for hA3A-nCas9 and gRNA expression in Aspergillus flavus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17843)
  AUTHORS   Zhao F, Sun C, Liu Z, Cabrera A, Escobar M, Huang S, Yuan Q, Nie Q, 
            Luo KL, Lin A, Vanegas JA, Zhu T, Hilton IB, Gao X
  TITLE     Multiplex Base-Editing Enables Combinatorial Epigenetic Regulation 
            for Genome Mining of Fungal Natural Products.
  JOURNAL   J Am Chem Soc. 2023 Jan 11;145(1):413-421. doi: 
            10.1021/jacs.2c10211. Epub 2022 Dec 21.
  PUBMED    36542862
REFERENCE   2  (bases 1 to 17843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.2c10211"; journalName: "J Am Chem Soc"; date: 
            "2023-01-11- 11"; volume: "145"; issue: "1"; pages: "413-421"
COMMENT     Sequence Label: pafG-CBE-3T
FEATURES             Location/Qualifiers
     source          1..17843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     109..126
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    243..264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(279..284,285..302,303..309)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    317..333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     322..344
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     341..357
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     341..357
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      374..1716
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(1337..1384)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     1757..1775
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1813..1835)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1935..1954
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1978..2193
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             2194..2997
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(3162..3750)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3242..3261)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3921..4712,4713..4781))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4544..4563
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4782..4886)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7796..7817
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_RNA        8929..9004
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        9100..9175
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        9271..9346
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             12236..12256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12266..12514
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             12545..13138
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="EASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTSV
                     KMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSWG
                     CAGEVRAFLQENTHVRLRIFAARIFDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWDT
                     FVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             join(13235..13258,13259..13261,13262..17335)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             17339..17359
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
       61 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
      121 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      181 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      241 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      301 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      361 gattacgcca ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag
      421 agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc
      481 gaaagcgcta ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac
      541 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
      601 aacgcgagag cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat
      661 gcatcccgag agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt
      721 gcgctctata atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag
      781 aaggctactt tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt
      841 ttactgatta ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat
      901 attctatacc gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt
      961 cttcattggt cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata
     1021 ggaaatgttt acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat
     1081 ttttttgtct aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga
     1141 tgcaagttca aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata
     1201 tatagcaaag agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta
     1261 gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg
     1321 ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     1381 cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca
     1441 gctcactgtt cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa
     1501 gaacggcata gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga
     1561 tgaaaggtag tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct
     1621 tccttcagca ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc
     1681 atccttcaat gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat
     1741 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
     1801 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     1861 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     1921 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ccaccttttc
     1981 aattcatcat ttttttttta ttcttttttt tgatttcggt ttccttgaaa tttttttgat
     2041 tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat
     2101 atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac
     2161 agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt
     2221 gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa
     2281 acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa
     2341 gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc
     2401 atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc
     2461 gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta
     2521 tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt
     2581 gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg
     2641 ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt
     2701 gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt
     2761 ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag
     2821 ggagacgcat tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac
     2881 attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa
     2941 cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa
     3001 actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta
     3061 tatcagttat taccctatgc ggtgtgactc tctacatatg acatgtgagc aaaaggccag
     3121 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3181 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3241 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3301 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3361 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3421 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3481 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3541 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3601 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3661 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3721 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3781 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3841 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3901 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3961 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4021 gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct
     4081 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4141 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4201 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4261 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4321 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4381 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4441 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4501 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4561 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4621 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4681 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4741 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4801 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4861 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgct
     4921 agctgtacaa aaaagcaggc tttaaaggaa ccaattcagt cgactggatc cggtaccaag
     4981 gtcgggcagg aactgcagat tttgacctag ttaacaaggc acatcatgct atcacttcca
     5041 acactcttgc tgagcgcgtt ctgccttgcc tttctggttc tacgaagtat ccagtcccgc
     5101 ctgcaacgcc gcagaaacgc caaaaggtgg ggttgtcaac cgccagctag agctccatcg
     5161 ggattctttg gcattccgcc gttcctcggt ggtcgcagat aactccctaa attccctgag
     5221 ctaactctaa gtcgaccatg ccgtttatgg ttagcgcctc ccaaaaagga atggccgact
     5281 tagagttacc tcttgaccga ctttttcttt cctccccctt acatttcgtt accacaacac
     5341 attcctatat ccaaactcca ggtacataac tagtcgaaat ctcttttaaa tctagtcaag
     5401 aactagatac ttaaccttca tactgcttag agatagcctt tgaagctctt atttcgctct
     5461 ttgtctcact ctcaccttcc tcctcctcct ccaatccttt ttgctcggga ctagtccaag
     5521 actagtcaac gattagtatg ccgagcattc gcgataacga tctccgaaag tccccagagt
     5581 actgtcacta tctcgaggca gttaaggacg gggagcttac gctgccggat ttcaagatag
     5641 taagccgacc cgactagctt taagcctagt tactgactag tccacaggac gacaatggcg
     5701 tgcctgatat ccatccatat gaagtctact gccgagtgaa gggatgcctc aagcgtacag
     5761 tgagtctact gctatactag tttctgacta gttattaact agttgacagg ttccctctgc
     5821 caacagaaac atattggtca agcacttgaa ggacaagaac tcccacggca tggagtttac
     5881 attgcacaat ggacctccca ctatgaagga actgatggag gccaaaggca agtcctatct
     5941 agattacttg gtgactagtc cctgactagt caagtagcat ggtatgaagg cttgtttgaa
     6001 ggcactgttc tcccaacccc gactcctacc aagaagcgca agcgagctgc gtaagtttct
     6061 gtgagtctaa ctagtgtatt agctaatata tagcagcacc aagtccaagg accacaatac
     6121 taagggtgtc gagaattcgt gagtttcttc tcccatttca actagtcctt gactagtcac
     6181 taactacttt gcagcaacga gggtgaagct ggaaatgatc aggacaatgg cgagggcccg
     6241 taagtacagc cattcaatgc agactagttg ctaactaatc tgtgactagt tcaagtggtc
     6301 cgtacgccgt gcatacccct gtgactggta ggaatttgag caagcctgtc ttgccgcgcg
     6361 atgagaaagg aaaggcaagt tacattcagc cccgtaccta ggatcagtgc taatttataa
     6421 cctctagcca ctctttatgc agatccgccg tgagggtagc aaggcagcta aatcagctgg
     6481 tgagaaagga accataccct gcaagacctg tcgcaacgca aagggcaaag gtaagctatc
     6541 caagctagtt tgggactaga ttctaactag tctcagcacc gtgtggttca aagccatatt
     6601 gcgagttttg gcgctttttc tcatcgattg acgaggcaaa gggagcgagt atgcaacctc
     6661 gtaagtcaga ctcagacaaa gaccaactag ttattgacca gtcttctaga aggctctgtt
     6721 gtggatcttg aggccctgga gagttcctcc aacaatccgg agacaagcaa gtcctcgtcg
     6781 gactagtcac taactagact ctaactagtt gcagacatgg ataatgcaaa agagacaagc
     6841 aatgaagaaa gtggtaagac atttctcctt tgtggttctg gactagtctt tgactagtca
     6901 cagtcttaaa caaggaaaat gagcatgaaa atgaggagga aaaggctgct gagcccgagg
     6961 aagtgcaggg tgatggcaga catggtaggt taataccttg ttagttattg ctagtcactg
     7021 actagtcaat aactagtctc tgaacacctt gcaatcactc cgtttgcgca gctgaacagt
     7081 ggtgaggata atagtagtaa gttattctag cttcagagtt ataggagact agatactaac
     7141 tagtattagt tgcaactaac ctggatctca gagactttgg cctcaatcta gaatctatct
     7201 agttgtcaac tagactgtgg tatcattgtc ttttattttc ctagtcctgg aactagcttc
     7261 taactagtct ccctaatatg tggctgtctt gttttttttt ttttgtttcc ctacccggat
     7321 atctagtccc cttctaggtt ctgttaacct ctcgggctct gatttagttt aacgcaaacc
     7381 tgagattagt ttctaactag tctctaggtt ttctatccac ctttaattgt aataataaat
     7441 acaagcaacg tttatacgtc aaaagcattt ataaactttt accctaaagt agcttgcttg
     7501 tgtgtttagt ttataattag tctcttatta atttgatgta ggtaagcccg ccacaaatat
     7561 atatttttaa caagataccg tggaaaaact tcgtgctatc acaaaacagt atacaaaaaa
     7621 taagctggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7681 gctttactgc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     7741 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7801 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     7861 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7921 gcgaatttta acaaaatggg gtgacgatga gccgctcttg catctttgtt tgtattatac
     7981 tgtctttctt gttacacata attattctag aatgccccac cgttacatac gggacacagc
     8041 catttacata tgcatgtgga ttacgagcta acgagttcat tcaaatctca gaactatcac
     8101 ataatcatca ttcccctatc gtcaaagacc gtaagacaaa tccggttcat gcactgaacc
     8161 cattcgggta gtgagtcatt tactcagcac actcgcgctg acgctcgtcg aacaccttca
     8221 atgcctcctc ggcagccttg acaccactga gaaccatggc accgaaggta gggcccatgc
     8281 ggttaaagcc atcaatttca gacagctcca taccgccgat tatcaagccc ttagtaacct
     8341 cgcgggtgtt cttgacgatg gcatcctgcg atcacataga tgctcggttg acaggaccac
     8401 ctatagcaag tactttgtag gaggttctcc taacgcttgg cttcaaatcc aatcaggata
     8461 tcagtgagtt actgcttgtc atcgcatcct catgaagtcg ctcaacagcc actgagaagc
     8521 aaatatttgg gagaccatcc ctgatgttga aattttacgc tggagcccat tcaccggtga
     8581 gctcggggac agtctggcca gtggagcgga aaatcttcaa ctaactctga ttggtaccac
     8641 aggtagcccc acagaacaaa ccgagcaaac atgaaaattt tcgcttgagg ttagcgcact
     8701 cgctagcgcc tgcccgcaaa tataaaaggc cccgaaatcc catgcatttt ggaaatcaag
     8761 cgactctacg tatgtcatcc ccctccctac ctttgggaaa acctctcaac tgcaagtcag
     8821 aacattttgc taacagcgca tcattggtct agtggtagaa ttcatcgttg ccatcgatga
     8881 ggcccgtgtt cgattcacgg atgatgcatg gtactcaagc tggggatggt tttagagcta
     8941 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     9001 gtgcttttgc atcattggtc tagtggtaga attcatcgtt gccatcgatg aggcccgtgt
     9061 tcgattcacg gatgatgcag ttccaagggg taggttgaag ttttagagct agaaatagca
     9121 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttg
     9181 catcattggt ctagtggtag aattcatcgt tgccatcgat gaggcccgtg ttcgattcac
     9241 ggatgatgca ggagctcagc acaccggaat gttttagagc tagaaatagc aagttaaaat
     9301 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt gcatcattgg
     9361 tctagtggta gaattcatcg ttgccatcga tgaggcccgt gttcgattca cggatgatgc
     9421 atttttttac gattaaacac atcgaagcag ggcctcaaac aatgctcttc accctcttcg
     9481 cgggtctgaa ataccctcac ctggcaacag caattggcgc ttcatggctg tttttccgat
     9541 ctctctactt gtacggctat gtgtactcgg gtaagccaca aggcaagggc agattgctgg
     9601 gaggtttctt ctggttttct caaggcgctc tgtgggctct gagtgtgttt ggtgttgcca
     9661 aagacatgat ctcttactga gagttattct gtgtctgacg aaatatgttg tgtatatata
     9721 tatatgtacg ttaaaagttc cgtggagtta ccagtgattg accaatgttt tatcttctac
     9781 agttctgcct gtctacccca ttctagctgt acctgactac agagtagttt aattgtggtt
     9841 gaccccacag tcggaggcgg aggaatacag caccgatgtg gcctgtctcc atccagattg
     9901 gcacgcaatt tttacacgcg gaaaagatcg agatagagta cgactttaaa tttagtcccc
     9961 ggcggcttct attttagaat atttgagatt tgattctcaa gcaattgatt tggttgggtc
    10021 accctcaatt ggataatata cctcattgct cggctacttc aactcatcaa tcaccgtcat
    10081 accccgcata taaccctcca ttcccacgat gtcgtccaag tcgcaattga cttacggtgc
    10141 tcgagccagc aagcacccca atcctctggc aaagagactt tttgagattg ccgaagcaaa
    10201 gaagacaaac gttaccgtct ctgctgatgt gacgacaacc cgagaactcc tggacctcgc
    10261 tgaccgtacg gaagctgttg gatccaatac atatgccgtc tagcaatgga ctaatcaact
    10321 tttgatgata caggtctcgg tccctacatc gccgtcatca agacacacat cgacatcctc
    10381 accgatttca gcgtcgacac tatcaatggc ctgaatgtgc tggctcaaaa gcacaacttt
    10441 ttgatcttcg aggaccgcaa attcatcgac atcggcaata ccgtccagaa gcaataccac
    10501 ggcggtgctc tgaggatctc cgaatgggcc cacattatca actgcagcgt tctccctggc
    10561 gagggcatcg tcgaggctct ggcccagacc gcatctgcgc aagacttccc ctatggtcct
    10621 gagagaggac tgttggtcct ggcagagatg acctccaaag gatcgctggc tacgggcgag
    10681 tataccaagg catcggttga ctacgctcgc aaatacaaga acttcgttat gggtttcgtg
    10741 tcgacgcggg ccctgacgga agtgcagtcg gatgtgtctt cagcctcgga ggatgaagat
    10801 ttcgtggtct tcacgacggg tgtgaacctc tcttccaaag gagataagct tggacagcaa
    10861 taccagactc ctgcatcggc tattggacgc ggtgccgact ttatcatcgc cggtcgaggc
    10921 atctacgctg ctcccgaccc ggttgaagct gcacagcggt accagaaaga aggctgggaa
    10981 gcttatatgg ccagagtatg cggcaagtca tgatttcctc ttggagcaaa agtgtagtgc
    11041 cagtacgagt gttgtggagg aaggctgcat acattgtgcc tgtcattaaa cgatgagctc
    11101 gtccgtattg gcccctgtaa tgccatgttt tccgccccca atcgtcaagg ttttcccttt
    11161 gttagattcc taccagtcat ctagcaagtg aggtaagctt tgccagaaac gccaaggctt
    11221 tatctatgta gtcgataagc aaagtggact gatagcttaa tatggaaggt ccctcaggac
    11281 aagtcgacct gtgcagaaga gataacagct tggcatcacg catcagtgcc tcctctcaga
    11341 cagaacgaga cagcagaatc accgcccaag ttaagccttt gtgctgatca tgctctcgaa
    11401 cgggccaagt tcgggaaaag caaaggagcg tttagtgagg ggcaatttga ctcacctccc
    11461 aggcaacaga tgaggggggc aaaaagaaag aaattttcgt gagtcaatat ggattccgag
    11521 catcattttc ttgcggtcta tcttgctacg tatgttgatc ttgacgctgt ggatcaagca
    11581 acgccactcg ctcgctccat cgcaggctgg tcgcagacaa attaaaaggc ggcaaactcg
    11641 tacagccgcg gggttgtccg ctgcaaagta cagagtgata aaagccgcca tgcgaccatc
    11701 aacgcgttga tgcccagctt tttcgatccg agaatccacc gtagaggcga tagcaagtaa
    11761 agaaaagcta aacaaaaaaa aatttctgcc cctaagccat gaaaacgaga tggggtggag
    11821 cagaaccaag gaaagagtcg cgctgggctg ccgttccgga aggtgttgta aaggctcgac
    11881 gcccaaggtg ggagtctagg agaagaattt gcatcgggag tggggcgggt tacccctcca
    11941 tatccaatga cagatatcta ccagccaagg gtttgagccc gcccgcttag tcgtcgtcct
    12001 cgcttgcccc tccataaaag gatttcccct ccccctccca caaaattttc tttcccttcc
    12061 tctccttgtc cgcttcagta cgtatatctt cccttccctc gcttctctcc tccatccttc
    12121 tttcatccat ctcctgctaa cttctctgct cagcacctct acgcattact agccgtagta
    12181 tctgagcact tctccctttt atattccaca aaacataaca cgcggccgca tgggacctaa
    12241 gaaaaagagg aaggtggcgg ccgctactaa tctgagcgac atcattgaga aggagactgg
    12301 gaaacagctg gtcattcagg agtccatcct gatgctgcct gaggaggtgg aggaagtgat
    12361 cggcaacaag ccagagtctg acatcctggt gcacaccgcc tacgacgagt ccacagatga
    12421 gaatgtgatg ctgctgacct ctgacgcccc cgagtataag ccttgggccc tggtcatcca
    12481 ggattctaac ggcgagaata agatcaagat gctgagcgga ggatccggag gatctggagg
    12541 cagcgaagcc agcccagcat ccgggcccag acacttgatg gatccacaca tattcacttc
    12601 caactttaac aatggcattg gaaggcataa gacctacctg tgctacgaag tggagcgcct
    12661 ggacaatggc acctcggtca agatggacca gcacaggggc tttctacaca accaggctaa
    12721 gaatcttctc tgtggctttt acggccgcca tgcggagctg cgcttcttgg acctggttcc
    12781 ttctttgcag ttggacccgg cccagatcta cagggtcact tggttcatct cctggagccc
    12841 ctgcttctcc tggggctgtg ccggggaagt gcgtgcgttc cttcaggaga acacacacgt
    12901 gagactgcgt atcttcgctg cccgcatctt cgattacgac cccctatata aggaggcact
    12961 gcaaatgctg cgggatgctg gggcccaagt ctccatcatg acctacgatg aatttaagca
    13021 ctgctgggac acctttgtgg accaccaggg atgtcccttc cagccctggg atggactaga
    13081 tgagcacagc caagccctga gtgggaggct gcgggccatt ctccagaatc agggaaactc
    13141 tggaggatct agcggaggat cctctggcag cgagacacca ggaacaagcg agtcagcaac
    13201 accagagagc agtggcggca gcagcggcgg cagcgacaag aagtatagca tcgggctggc
    13261 cattggaacg aactcggttg gttgggctgt gattacggac gaatacaagg tgccatccaa
    13321 gaagtttaag gtcctgggaa acaccgaccg tcactcaatc aagaagaatc tcattggagc
    13381 cctgctcttc gatagtgggg agaccgccga agctactcga ctgaagcgaa cggctcgccg
    13441 gcgttataca cgacgcaaga atcgcatctg ctacctccag gagattttca gcaacgaaat
    13501 ggctaaggtt gatgactcat tctttcatcg actcgaagaa agtttcttgg tcgaggagga
    13561 taagaagcac gagcgccatc cgatctttgg taacattgtg gatgaggttg cctatcacga
    13621 aaagtaccca actatctatc atcttcgtaa gaagctggtc gatagcacgg acaaggctga
    13681 tttgcgactt atctacctgg cactcgcgca catgattaag ttccgcggcc attttcttat
    13741 cgagggtgac ctgaaccccg ataattctga cgttgataag ctcttcatcc agttggtcca
    13801 aacctacaat cagctgtttg aggaaaaccc tattaatgca tctggcgtgg acgccaaggc
    13861 tatcctttcg gcgcgcctgt ctaagtcgcg gcgtttggag aaccttatcg cacaactccc
    13921 cggcgaaaag aagaacggcc tcttcggtaa tttgattgcg ttgtcacttg gtctgactcc
    13981 taacttcaag agtaattttg acctggcaga ggatgcgaag ctccagttgt ctaaggatac
    14041 gtatgatgac gatctcgaca acttgcttgc ccaaatcggt gaccagtacg ctgatctttt
    14101 cctggccgct aagaatctct cagatgcaat cctgctcagt gacattttgc gggtcaacac
    14161 cgagattact aaggcccccc tgtcagctag tatgatcaag cggtatgatg agcaccatca
    14221 ggacctcacc ttgcttaagg ccctcgtgcg tcagcaattg cctgagaagt ataaggaaat
    14281 cttctttgac caatccaaga acggatacgc agggtatatt gatggcggtg cgagccagga
    14341 ggaattctac aagtttatca agccgatttt ggagaagatg gacggcactg aggaactgct
    14401 cgtcaagctg aatcgcgaag atttgcttcg taagcaacga acgttcgaca acggctccat
    14461 cccgcaccag attcatctgg gcgagctcca cgccatcctt cgacgccagg aagatttcta
    14521 cccatttctg aaggacaacc gtgagaagat cgaaaagatt cttacattcc gaatccccta
    14581 ctatgtggga cctttggccc gtgggaattc ccgatttgct tggatgaccc gaaagagcga
    14641 ggaaaccatc actccgtgga acttcgagga agtcgtggac aagggtgcat ccgcgcagag
    14701 cttcattgag cggatgacca attttgataa gaaccttccg aatgaaaagg tcctgccaaa
    14761 gcattcgctg ctctacgagt atttcaccgt gtataacgaa ctgactaagg tcaagtacgt
    14821 gacggaggga atgcggaagc cagccttcct ctcaggggaa caaaagaagg ctatcgtcga
    14881 tttgcttttt aagaccaatc gtaaagtgac tgttaagcag ctgaaggagg attatttcaa
    14941 gaagattgaa tgtttcgact ccgtcgagat cagcggcgtg gaagatcgct ttaacgcttc
    15001 cctcggtacc taccacgacc tgctcaagat cattaaggac aaggatttcc tcgataacga
    15061 ggaaaatgag gacatcttgg aagatattgt cctcacgttg acactttttg aggaccgcga
    15121 aatgatcgag gaacggctca agacatatgc ccatttgttc gacgataagg tgatgaagca
    15181 gctgaagcgg cgtcgataca ccggatgggg tcgccttagc cggaagctga tcaacggcat
    15241 tcgagataag caatctggta agactatctt ggatttcctt aagtcggacg gcttcgccaa
    15301 ccgcaatttt atgcagctta ttcacgacga ttccctgacg ttcaaggagg acatccagaa
    15361 ggcacaagtc tcaggacaag gggattccct gcacgagcat atcgccaacc tggctggatc
    15421 cccggcgatc aagaagggga ttcttcagac cgtcaaggtt gtcgacgagc tggtcaaggt
    15481 gatgggccgt cataagccag aaaacatcgt gattgagatg gcccgagaaa atcagaccac
    15541 tcaaaagggt cagaagaaca gccgcgagcg gatgaagcgg atcgaggaag gcattaagga
    15601 acttggttct cagatcctga aggagcaccc tgttgaaaac acacagctcc aaaatgagaa
    15661 gctgtatctc tactatttgc aaaatggacg cgacatgtac gtcgatcagg agctcgacat
    15721 taaccggttg tcggactacg atgttgacca tatcgtcccg caatccttcc ttaaggacga
    15781 tagcattgat aacaaggtgc tgactcgctc agataagaac cggggcaagt ccgacaatgt
    15841 tccaagcgag gaagtggtta agaagatgaa gaactactgg cgccaattgc ttaatgccaa
    15901 gctcatcaca cagcgcaagt ttgacaactt gaccaaggcc gagcggggag ggctgagtga
    15961 actcgataag gctggcttca tcaagcgtca actcgtggag acgcgacaga tcacaaagca
    16021 cgttgctcag attctggact cccggatgaa cacaaagtac gacgagaatg ataagctcat
    16081 ccgtgaagtt aaggtcatta ccctcaagtc taagttggtg tcggatttcc gcaaggactt
    16141 ccaattttat aaggttcggg agatcaacaa ttatcaccat gcacatgatg cgtacctcaa
    16201 cgcagtcgtg ggaactgcgc tcatcaagaa gtatcccaag ttggagtccg aattcgtcta
    16261 cggggattat aaggtttacg acgtccgcaa gatgatcgcc aagagtgagc aggaaattgg
    16321 caaggccacg gctaagtatt tcttttactc caacatcatg aatttcttta agacggagat
    16381 cacactcgcc aatggagaaa tccgtaagcg acctttgatt gagaccaacg gcgagactgg
    16441 tgaaatcgtt tgggataagg ggcgcgactt cgctaccgtg cggaaggttc tgagcatgcc
    16501 gcaagtcaat atcgtcaaga aaaccgaggt gcagacaggc ggtttctcta aggaatcgat
    16561 tcttccaaag cgtaactctg acaagctgat cgctcgaaag aaggattggg accccaagaa
    16621 gtatggaggg ttcgattctc ctacagtggc atactcggtt ctcgttgtcg cgaaggttga
    16681 gaagggaaag tctaagaagc tgaagtcggt caaggaactg ctcgggatca ccattatgga
    16741 gcgctccagc ttcgaaaaga atcccatcga ctttctcgag gccaagggct ataaggaagt
    16801 caagaaggat cttatcatta agctgcctaa gtactctttg ttcgagcttg aaaacggtcg
    16861 aaagcgaatg ctcgcatcgg caggagagtt gcagaagggg aatgaattgg cacttccctc
    16921 aaagtacgtg aacttcctgt atctcgcgtc ccactacgag aagctgaagg gtagccctga
    16981 ggacaacgaa cagaagcaac tttttgttga gcaacacaag cattatctgg atgagatcat
    17041 tgaacagatt tcagagttca gtaagcgcgt catcctcgcc gatgctaatc tcgacaaggt
    17101 gttgtcggcc tacaacaagc accgtgacaa gccgatccga gagcaggctg aaaatatcat
    17161 tcatctgttc accctcacta acttgggagc accagcagcg ttcaagtatt ttgatacgac
    17221 aatcgaccgt aagcgataca cgtccacaaa ggaggtgctt gatgcgaccc tgattcatca
    17281 atccatcact gggctctatg aaacccgtat cgaccttagt caactggggg gcgaccctcc
    17341 caagaagaag cgcaaggtct gagcggacat tcgatttatg ccgttatgac ttccttaaaa
    17401 aagcctttac gaatgaaaga aatggaatta gacttgttat gtagttgatt ctacaatgga
    17461 ttatgattcc tgaacttcaa atccgctgtt cattattaat ctcagctctt cccgtaaagc
    17521 caatgttgaa actattcgta aatgtacctc gttttgcgtg taccttgctt atcacgtgat
    17581 attacatgac ctggacagag ttctgcgcga aagtcataac gtaaatcccg ggcggtaggt
    17641 gcgtcccggg cggaaggtag ttttctcgtc caccccaacg cgtttatcaa cctcaacttt
    17701 caacaaccat catgccacca aaagcgcgta aaacaaagcg agatttgatt gagcaagagg
    17761 gcaggatcca atgcgcgatt caagacatta aaaatggaaa atttcaaaaa attgcgcccg
    17821 cagcgcgtgc atacaaaatt cat
//
