LOCUS       sequence-440054-       11191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Foxp1A in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Bergmann T, Liu Y, Skov J, Mogus L, Lee J, Pfisterer U, Handfield 
            LF, Asenjo-Martinez A, Lisa-Vargas I, Seemann SE, Lee JTH, Patikas 
            N, Kornum BR, Denham M, Hyttel P, Witter MP, Gorodkin J, Pers TH, 
            Hemberg M, Khodosevich K, Hall VJ
  TITLE     Production of human entorhinal stellate cell-like cells by forward 
            programming shows an important role of Foxp1 in reprogramming.
  JOURNAL   Front Cell Dev Biol. 2022 Aug 15;10:976549. doi: 
            10.3389/fcell.2022.976549. eCollection 2022.
  PUBMED    36046338
REFERENCE   2  (bases 1 to 11191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2022-08-15"; volume: "10:976549. doi"; pages: " 
            10.3389/fcell.2022.976549. eCollection 2022"
COMMENT     Sequence Label: p-Tet-O-Foxp1A-Puro
FEATURES             Location/Qualifiers
     source          1..11191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        100..479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        481..679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             697..877
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    924..1049
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1542..1775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1960..2004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2302..2419
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2476..2494,2495..2517,2518..2536,2537..2559,2560..2578,
                     2579..2601,2602..2620,2621..2643,2644..2662,2663..2685,
                     2686..2704,2705..2727,2728..2746)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2779..2817
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2814..2838
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5047..5100
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5107..5706
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5107..5126)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5603..5623
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5745..6333
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5798..5818)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6216..6227)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6336..6352)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6337..6353)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6858..7038
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7064..7081)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7070..7294
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7340..7768
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7427..7446)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7637..7658
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7777..7797)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7782..8111
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7962..8097
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8024..8043
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8159..8206
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8225..8599
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8729..8850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8766..8785)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8820..8839
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8899..8915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8899..8915)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8912..8934)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8923..8939
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8947..8953,8954..8971,8972..8977))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8992..9013
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9130..9147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9301..9889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9381..9400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10060..10851,10852..10920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10683..10702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10921..11025)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11100..11119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
       61 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      121 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      181 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      241 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      301 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      361 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      421 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      481 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      541 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      601 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      661 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      721 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      781 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      841 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      901 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      961 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     1021 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     1081 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     1141 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     1201 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     1261 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     1321 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     1381 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     2041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     2101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     2161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     2221 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     2281 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2341 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2401 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaga
     2461 tctcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2521 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2581 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     2641 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     2701 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2761 gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
     2821 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     2881 caccgggacc gatccagcct ccgcggcccc gaattcgcca ccatgcaaga atctgggtct
     2941 gagacaaaaa gtaacggatc ggccatccag aacgggtcca gcggtggcaa ccacttacta
     3001 gagtgcgggg cacttcgtga cactcggtcc aacggagagg caccagcggt ggacctgggg
     3061 gcagccgacc ttgcccacgt ccagcagcag caacagcagg ccctgcaggt ggcaaggcag
     3121 ctcctccttc agcagcagca acagcagcag cagcaacagc agcagcagca gcagcagcaa
     3181 cagcagcaac agcagcagca acaacagcag cagcagcaac agcagcagca gcagcagcaa
     3241 gttagtggat taaagtctcc caagaggaat gacaagcaac cagctcttca ggttcccgtg
     3301 tcagtggcta tgatgacacc tcaggttatc actcctcaac aaatgcagca gatcctccag
     3361 cagcaggtgc tgagccccca gcagctccag gttctcctcc agcagcagca ggccctcatg
     3421 cttcaacagc agcagcttca agaattttat aaaaaacaac aggaacagtt gcagcttcaa
     3481 cttctccaac agcaacatgc tggaaaacag ccgaaagagc agcagcaggt ggctacccag
     3541 cagttggctt tccagcagca gcttctccag atgcagcagc tgcagcagca gcacctcttg
     3601 tctctccagc gccaaggcct cctaacaatt cagccggggc agcctgccct tcccctccaa
     3661 cccctcgctc aaggcatgat tccaacggaa ctgcagcagc tctggaaaga agtgacaagt
     3721 gcccacactg cagaggaaac cacaagcagc aaccacagca gcctagacct gaccagcaca
     3781 tgtgtctcgt cctcggcacc ttccaagtcc tccctaatca tgaacccgca tgcctctacc
     3841 aatggacagc tctcggtcca cactcccaaa agggaaagct tgtcccacga ggagcacccc
     3901 cacagccacc ctctctatgg acatggcgta tgcaagtggc caggctgtga ggcggtttgt
     3961 gacgacttcc cagcctttct aaaacatctc aacagtgagc atgcgctgga cgatagaagc
     4021 acagctcaat gtagagtaca aatgcaggtt gtacagcagt tagagctaca gcttgcaaaa
     4081 gacaaagagc gcctgcaagc catgatgacc cacctgcatg tgaagtctac agaacccaaa
     4141 gctgcccctc agcccctgaa tctggtatca agtgtcaccc tctccaagtc tgcctcagag
     4201 gcttctccac agagcttacc tcatactcca acaaccccca ccgcccccct gactcctgtc
     4261 acccaaggcc cctccgtcat caccaccacc agcatgcaca cggtgggacc tatccgcagg
     4321 cggtactcag acaaatacaa cgtgcccatt tcttcagcag atattgcgca gaaccaagaa
     4381 ttttataaga acgcggaagt tagaccacca tttacatatg catctttaat caggcaggcc
     4441 attctcgaat ctccagaaaa gcagctaaca ctaaacgaaa tctataactg gttcacacga
     4501 atgtttgctt acttccgacg caatgcagcc acgtggaaga atgcagtgcg tcataatctt
     4561 agtctccaca agtgttttgt gcgagtagag aacgttaaag gggcagtatg gacagtggat
     4621 gaagtagagt tccaaaaacg gaggccacaa aagatcagtg gtaacccttc ccttattaaa
     4681 aacatgcaga gcagccacgc ctactgcaca cctctcaatg cagctttaca ggcttccatg
     4741 gctgagaata gtatacctct gtacactacc gcttccatgg gaaatcccac tctgggcagc
     4801 ctggccagtg ccatccggga ggagctgaac ggggccatgg agcacaccaa cagcaacgag
     4861 agtgacagca gtccaggcag atcccctatg caagctgtgc accccataca cgtcaaagaa
     4921 gaacccctcg accccgagga agctgaaggc cctctgtcct tagtgacaac agccaaccac
     4981 agtccagatt ttgaccatga cagagattac gaagacgaac cagtaaatga ggacatggag
     5041 tctagagagg gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca
     5101 ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5161 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5221 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5281 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     5341 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5401 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5461 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     5521 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     5581 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     5641 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     5701 gcctgattaa ttaagctagc taattcgata tcaagcttat cgataatcaa cctctggatt
     5761 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5821 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5881 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5941 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6001 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6061 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6121 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6181 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6241 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6301 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     6361 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     6421 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6481 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6541 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     6601 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     6661 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     6721 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     6781 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     6841 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6901 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6961 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7021 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     7081 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7141 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7201 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7261 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7321 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7381 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7441 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7501 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7561 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7621 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7681 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7741 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     7801 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7861 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7921 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7981 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8041 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8101 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     8161 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     8221 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     8281 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     8341 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     8401 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     8461 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     8521 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     8581 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     8641 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     8701 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     8761 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8821 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     8881 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8941 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9001 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9061 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10201 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10621 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10681 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10741 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10801 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10861 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10921 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11041 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
    11101 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
    11161 gtcgctgagt agtgcgcgag caaaatttaa g
//
