LOCUS       sequence-440074-        1374 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of FAM177A1-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Ugur B, Schueder F, Shin J, Hanna MG, Wu Y, Leonzino M, Su M, McAdow
            AR, Wilson C, Postlethwait J, Solnica-Krezel L, Bewersdorf J, De 
            Camilli P
  TITLE     VPS13B is localized at the cis-trans Golgi complex interface and is 
            a functional partner of FAM177A1.
  JOURNAL   bioRxiv [Preprint]. 2023 Dec 18:2023.12.18.572081. doi: 
            10.1101/2023.12.18.572081.
  PUBMED    38187698
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Dec 18:2023.12.18.572081. doi: 
            10.1101/2023.12.18.572081."
COMMENT     Sequence Label: FAM177A1isoform2-GFP
FEATURES             Location/Qualifiers
     source          1..1374
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      649..658
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(655..657,658..660,661..1374)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(655..657,658..660,661..1371)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(700..721)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(961..980)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1308..1329
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggaccagg agccagtggg cggtgtggaa cgaggagaag ccgtcgcagc ctcgggagct
       61 gcggccgccg cggcattcgg ggaatctgca gggcagatga gtaacgaaag aggctttgaa
      121 aatgtagaac tgggagtcat aggaaaaaag aagaaagtcc caaggagagt catccacttt
      181 gttagtggtg aaacaatgga agaatatagc acagatgaag acgaagttga tggcctggag
      241 aagaaagatg ttttgcctac tgttgatccg acaaaactta cctggggtcc ctacttatgg
      301 ttttacatgc ttcgggctgc tacatcaact ctctcagtgt gtgacttcct tggagagaag
      361 attgcatctg ttttgggtat cagcacccca aagtaccaat atgccattga tgaatattat
      421 cggatgaaga aggaggaaga agaagaagaa gaagaaaaca ggatgtctga agaagcagaa
      481 aaacaatatc aacagaataa attgcagact gattccattg ttcagacaga tcaaccagag
      541 acagtgatat ccagctcatt tgtgaatgtc aattttgaaa tggagggaga cagtgaagta
      601 attatggaaa gcaagcaaaa tccagtctct gtcccaccag aaccggtcgc caccatggtg
      661 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      721 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      781 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      841 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      901 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      961 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1021 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1081 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1141 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1201 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1261 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1321 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaa
//
