LOCUS       sequence-440075-        1614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of FAM177A1-Halo in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ugur B, Schueder F, Shin J, Hanna MG, Wu Y, Leonzino M, Su M, McAdow
            AR, Wilson C, Postlethwait J, Solnica-Krezel L, Bewersdorf J, De 
            Camilli P
  TITLE     VPS13B is localized at the cis-trans Golgi complex interface and is 
            a functional partner of FAM177A1.
  JOURNAL   bioRxiv [Preprint]. 2023 Dec 18:2023.12.18.572081. doi: 
            10.1101/2023.12.18.572081.
  PUBMED    38187698
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2023 Dec 18:2023.12.18.572081. doi: 
            10.1101/2023.12.18.572081."
COMMENT     Sequence Label: FAM177A1-Halo
FEATURES             Location/Qualifiers
     source          1..1614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             721..1611
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
ORIGIN
        1 atggaagtgg gcttaccggc cattaccctc tttctcacca gcgccagcag ccctgtggtg
       61 gcgacgacga tggaccagga gccagtgggc ggtgtggaac gaggagaagc cgtcgcagcc
      121 tcgggagctg cggccgccgc ggcattcggg gaatctgcag ggcagatgag taacgaaaga
      181 ggctttgaaa atgtagaact gggagtcata ggaaaaaaga agaaagtccc aaggagagtc
      241 atccactttg ttagtggtga aacaatggaa gaatatagca cagatgaaga cgaagttgat
      301 ggcctggaga agaaagatgt tttgcctact gttgatccga caaaacttac ctggggtccc
      361 tacttatggt tttacatgct tcgggctgct acatcaactc tctcagtgtg tgacttcctt
      421 ggagagaaga ttgcatctgt tttgggtatc agcaccccaa agtaccaata tgccattgat
      481 gaatattatc ggatgaagaa ggaggaagaa gaagaagaag aagaaaacag gatgtctgaa
      541 gaagcagaaa aacaatatca acagaataaa ttgcagactg attccattgt tcagacagat
      601 caaccagaga cagtgatatc cagctcattt gtgaatgtca attttgaaat ggagggagac
      661 agtgaagtaa ttatggaaag caagcaaaat ccagtctctg tcccaccagg aggaagtgga
      721 atggcagaaa tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag
      781 cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt
      841 aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc
      901 tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc
      961 ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc
     1021 gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca
     1081 gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa
     1141 tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag
     1201 ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg
     1261 ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaatcctgt tgaccgcgag
     1321 ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg
     1381 ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg
     1441 ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct
     1501 aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac
     1561 ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg agatttccgg ctga
//
